Enzyme-gold nanoparticle bioconjugates: Quantification of particle stoichiometry and enzyme specific activity

Jacqueline D. Keighron, Christine Dolan Keating

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations

Abstract

Enzyme-gold nanoparticle bioconjugates have a wide variety of uses ranging from nanoreactors to sensors and model systems. While easy to make, these bioconjugates are often not well characterized. This protocol outlines preparation of enzyme-nanoparticle bioconjugates and two complementary methods for quantifying enzyme:nanoparticle stoichiometry from which the specific activity of the adsorbed enzymes can be determined. Characterizations such as these can aid researchers in improving the design and application of future bioconjugate systems.

Original languageEnglish (US)
Title of host publicationNanoBiotechnology Protocols
Pages163-174
Number of pages12
DOIs
StatePublished - Sep 3 2013

Publication series

NameMethods in Molecular Biology
Volume1026
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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    Keighron, J. D., & Keating, C. D. (2013). Enzyme-gold nanoparticle bioconjugates: Quantification of particle stoichiometry and enzyme specific activity. In NanoBiotechnology Protocols (pp. 163-174). (Methods in Molecular Biology; Vol. 1026). https://doi.org/10.1007/978-1-62703-468-5-13