Enzyme immobilization on polymethacrylate-based monolith fabricated via thermally induced phase separation

Wenjuan Han; Yuanrong Xin; Urara Hasegawa; Hiroshi Uyama

doi:10.1016/j.polymdegradstab.2014.05.032

Enzyme immobilization on polymethacrylate-based monolith fabricated via thermally induced phase separation

Wenjuan Han, Yuanrong Xin, Urara Hasegawa, Hiroshi Uyama

Materials Science and Engineering

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Polymer-based monolithic materials with interconnected porous structure have received much attention as high-performance matrices for enzyme immobilization. The present work describes immobilization of horseradish peroxidase (HRP) onto a polymethacrylate-based monolith which was fabricated by thermally induced phase separation (TIPS) method. The poly(glycidyl methacrylate-co-methyl methacrylate) (PGM) monolith was modified with adipic acid dihydrazide (AADH) and ethylenediaminetetraacetic dianhydride (EDTAD) to yield carboxyl group-bearing PGM (PGM-COOH) monolith. This monolith was reacted with N-hydroxysuccinimide (NHS) to activate the carboxyl groups on the monolith and further modified with HRP. The immobilized HRP showed much higher thermal stability than the free one. Furthermore, the immobilized HRP could be reused for at least 6 times with the remaining activity of 52%. The PGM monolith may have a high potential as a solid support for enzyme immobilization.

Original language	English (US)
Pages (from-to)	362-366
Number of pages	5
Journal	Polymer Degradation and Stability
Volume	109
DOIs	https://doi.org/10.1016/j.polymdegradstab.2014.05.032
State	Published - Nov 2014

All Science Journal Classification (ASJC) codes

Condensed Matter Physics
Mechanics of Materials
Polymers and Plastics
Materials Chemistry

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10.1016/j.polymdegradstab.2014.05.032

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title = "Enzyme immobilization on polymethacrylate-based monolith fabricated via thermally induced phase separation",

abstract = "Polymer-based monolithic materials with interconnected porous structure have received much attention as high-performance matrices for enzyme immobilization. The present work describes immobilization of horseradish peroxidase (HRP) onto a polymethacrylate-based monolith which was fabricated by thermally induced phase separation (TIPS) method. The poly(glycidyl methacrylate-co-methyl methacrylate) (PGM) monolith was modified with adipic acid dihydrazide (AADH) and ethylenediaminetetraacetic dianhydride (EDTAD) to yield carboxyl group-bearing PGM (PGM-COOH) monolith. This monolith was reacted with N-hydroxysuccinimide (NHS) to activate the carboxyl groups on the monolith and further modified with HRP. The immobilized HRP showed much higher thermal stability than the free one. Furthermore, the immobilized HRP could be reused for at least 6 times with the remaining activity of 52%. The PGM monolith may have a high potential as a solid support for enzyme immobilization.",

author = "Wenjuan Han and Yuanrong Xin and Urara Hasegawa and Hiroshi Uyama",

note = "Funding Information: This study is financially supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (No. 21350124 ) and a Project for Creating Start-ups from Advanced Research and Technology , MEXT . Publisher Copyright: {\textcopyright} 2014 Elsevier Ltd. All rights reserved.",

year = "2014",

month = nov,

doi = "10.1016/j.polymdegradstab.2014.05.032",

language = "English (US)",

volume = "109",

pages = "362--366",

journal = "Polymer Degradation and Stability",

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AU - Han, Wenjuan

AU - Xin, Yuanrong

AU - Hasegawa, Urara

AU - Uyama, Hiroshi

N1 - Funding Information: This study is financially supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (No. 21350124 ) and a Project for Creating Start-ups from Advanced Research and Technology , MEXT . Publisher Copyright: © 2014 Elsevier Ltd. All rights reserved.

PY - 2014/11

Y1 - 2014/11

N2 - Polymer-based monolithic materials with interconnected porous structure have received much attention as high-performance matrices for enzyme immobilization. The present work describes immobilization of horseradish peroxidase (HRP) onto a polymethacrylate-based monolith which was fabricated by thermally induced phase separation (TIPS) method. The poly(glycidyl methacrylate-co-methyl methacrylate) (PGM) monolith was modified with adipic acid dihydrazide (AADH) and ethylenediaminetetraacetic dianhydride (EDTAD) to yield carboxyl group-bearing PGM (PGM-COOH) monolith. This monolith was reacted with N-hydroxysuccinimide (NHS) to activate the carboxyl groups on the monolith and further modified with HRP. The immobilized HRP showed much higher thermal stability than the free one. Furthermore, the immobilized HRP could be reused for at least 6 times with the remaining activity of 52%. The PGM monolith may have a high potential as a solid support for enzyme immobilization.

AB - Polymer-based monolithic materials with interconnected porous structure have received much attention as high-performance matrices for enzyme immobilization. The present work describes immobilization of horseradish peroxidase (HRP) onto a polymethacrylate-based monolith which was fabricated by thermally induced phase separation (TIPS) method. The poly(glycidyl methacrylate-co-methyl methacrylate) (PGM) monolith was modified with adipic acid dihydrazide (AADH) and ethylenediaminetetraacetic dianhydride (EDTAD) to yield carboxyl group-bearing PGM (PGM-COOH) monolith. This monolith was reacted with N-hydroxysuccinimide (NHS) to activate the carboxyl groups on the monolith and further modified with HRP. The immobilized HRP showed much higher thermal stability than the free one. Furthermore, the immobilized HRP could be reused for at least 6 times with the remaining activity of 52%. The PGM monolith may have a high potential as a solid support for enzyme immobilization.

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VL - 109

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JO - Polymer Degradation and Stability

JF - Polymer Degradation and Stability

ER -

Enzyme immobilization on polymethacrylate-based monolith fabricated via thermally induced phase separation

Abstract

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