Enzyme immobilization on polymethacrylate-based monolith fabricated via thermally induced phase separation

Wenjuan Han, Yuanrong Xin, Urara Hasegawa, Hiroshi Uyama

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Polymer-based monolithic materials with interconnected porous structure have received much attention as high-performance matrices for enzyme immobilization. The present work describes immobilization of horseradish peroxidase (HRP) onto a polymethacrylate-based monolith which was fabricated by thermally induced phase separation (TIPS) method. The poly(glycidyl methacrylate-co-methyl methacrylate) (PGM) monolith was modified with adipic acid dihydrazide (AADH) and ethylenediaminetetraacetic dianhydride (EDTAD) to yield carboxyl group-bearing PGM (PGM-COOH) monolith. This monolith was reacted with N-hydroxysuccinimide (NHS) to activate the carboxyl groups on the monolith and further modified with HRP. The immobilized HRP showed much higher thermal stability than the free one. Furthermore, the immobilized HRP could be reused for at least 6 times with the remaining activity of 52%. The PGM monolith may have a high potential as a solid support for enzyme immobilization.

Original languageEnglish (US)
Pages (from-to)362-366
Number of pages5
JournalPolymer Degradation and Stability
Volume109
DOIs
StatePublished - Nov 2014

All Science Journal Classification (ASJC) codes

  • Condensed Matter Physics
  • Mechanics of Materials
  • Polymers and Plastics
  • Materials Chemistry

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