Enzyme immunoassay for detection of human immunodeficiency virus-specific immunoglobulin A antibodies

E. Connor, Z. Wang, R. Stephens, B. Holland, P. Palumbo, George McSherry, J. Oleske, T. Denny

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Abstract

Early diagnosis of human immunodeficiency virus (HIV) infection may be difficult in adults with acute or recent HIV infection and in infants with perinatally acquired HIV. Detection of HIV-specific immunoglobulin A (IgA) antibodies in infant serum by Western blot (immunoblot) has been suggested as a reliable method to identify HIV-infected infants, especially those over the age of 6 months, and as an adjunct to diagnosis of acute HIV infection in adults. We developed a simple enzyme immunoassay for detection of HIV- specific IgA, using standard commercially available reagents. Enzyme immunoassay was comparable to Western blot for detection of HIV-specific IgA in sera from adults (n = 216), older children (n = 49), and infants born to HIV-infected mothers (n = 65). Specificity was 100% and sensitivity ranged from 80 to 92%. IgA-enzyme immunoassay is a simple, highly sensitive method for detection of HIV-specific IgA antibodies and is easily adapted to the standard clinical laboratory.

Original languageEnglish (US)
Pages (from-to)681-684
Number of pages4
JournalJournal of clinical microbiology
Volume31
Issue number3
Publication statusPublished - Jan 1 1993

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All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Connor, E., Wang, Z., Stephens, R., Holland, B., Palumbo, P., McSherry, G., ... Denny, T. (1993). Enzyme immunoassay for detection of human immunodeficiency virus-specific immunoglobulin A antibodies. Journal of clinical microbiology, 31(3), 681-684.