Enzyme immunoassay for detection of human immunodeficiency virus-specific immunoglobulin A antibodies

E. Connor, Z. Wang, R. Stephens, B. Holland, P. Palumbo, G. McSherry, J. Oleske, T. Denny

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Early diagnosis of human immunodeficiency virus (HIV) infection may be difficult in adults with acute or recent HIV infection and in infants with perinatally acquired HIV. Detection of HIV-specific immunoglobulin A (IgA) antibodies in infant serum by Western blot (immunoblot) has been suggested as a reliable method to identify HIV-infected infants, especially those over the age of 6 months, and as an adjunct to diagnosis of acute HIV infection in adults. We developed a simple enzyme immunoassay for detection of HIV- specific IgA, using standard commercially available reagents. Enzyme immunoassay was comparable to Western blot for detection of HIV-specific IgA in sera from adults (n = 216), older children (n = 49), and infants born to HIV-infected mothers (n = 65). Specificity was 100% and sensitivity ranged from 80 to 92%. IgA-enzyme immunoassay is a simple, highly sensitive method for detection of HIV-specific IgA antibodies and is easily adapted to the standard clinical laboratory.

Original languageEnglish (US)
Pages (from-to)681-684
Number of pages4
JournalJournal of clinical microbiology
Volume31
Issue number3
DOIs
StatePublished - 1993

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

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