Ovine interferon-π (OvIFN-π) is a trophectoderm secretory protein responsible for maintenance of corpus luteum function during early pregnancy. Methods for its quantitation include an antiviral assay and radioimmunoassay (RIA), both of which have disadvantages. We therefore developed an ELISA for OvIFN-π that is specific, rapid, and 40-fold more sensitive than the current RIA. It uses a monoclonal antibody (Ab) HL129, which binds both native and recombinant (r) OvIFN-π. The ELISA accurately detected known amounts of rOvIFN-π added to yeast and tissue culture medium and to sheep serum at concentrations between 0.005 and 50,000 ng/ml. Inter- and intraassay coefficients of variation were 9.3 ± 0.8 and 6.1 ± 1.4%, respectively. Data are given for the reliability and reproducibility of the ELISA for measuring native OvIFN-π and rOvIFN-π.
All Science Journal Classification (ASJC) codes
- Cell Biology