Enzyme molecules as nanomotors

Samudra Sengupta, Krishna K. Dey, Hari S. Muddana, Tristan Tabouillot, Michael E. Ibele, Peter J. Butler, Ayusman Sen

Research output: Contribution to journalArticlepeer-review

169 Scopus citations

Abstract

Using fluorescence correlation spectroscopy, we show that the diffusive movements of catalase enzyme molecules increase in the presence of the substrate, hydrogen peroxide, in a concentration-dependent manner. Employing a microfluidic device to generate a substrate concentration gradient, we show that both catalase and urease enzyme molecules spread toward areas of higher substrate concentration, a form of chemotaxis at the molecular scale. Using glucose oxidase and glucose to generate a hydrogen peroxide gradient, we induce the migration of catalase toward glucose oxidase, thereby showing that chemically interconnected enzymes can be drawn together.

Original languageEnglish (US)
Pages (from-to)1406-1414
Number of pages9
JournalJournal of the American Chemical Society
Volume135
Issue number4
DOIs
StatePublished - Jan 30 2013

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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