Epigallocatechin-3-gallate suppresses neutrophil migration speed in a transgenic zebrafish model accompanied by reduced inflammatory mediators

Thao Nguyen, Brittany Payan, Amarayca Zambrano, Yong Du, Maria Bondesson, Chandra Mohan

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Background: Polyphenol catechins from green tea, particularly (-)-epigallocatechin-3-gallate(EGCG), exhibits numerous beneficial health effects, although the mechanisms remain unclear. Methods: In this study, the mechanism of EGCG-mediated healing in an experimentally injured zebrafish model was examined at the cellular and molecular level using confocal microscopy and gene expression analysis. Results: The mechanisms of action of EGCG were shown to involve: (1) reducing neutrophil response (accumulation, travel speed, and distance) and (2) downregulating the expression of IL-1β, TNFα, and related signaling pathways. As determined by dynamic time-lapse tracking studies, the local accumulation of neutrophils with high migration speeds after wounding (n=33 cells, v=0.020 µm/s, d=37.8 µm), underwent significant reduction following treatment with EGCG doses of 300 µM (n=22 cells, v=0.013 µm/s, d=39.5 µm) and 600 µM (n=18 cells, v=0.008 µm/s, d=9.53 µm). Reverse transcription polymerase chain reaction studies revealed that several signature genes in the IL-1β, TNFα, and related signaling pathways were downregulated after EGCG treatment. Conclusion: The convenience, transparency, and simplicity of the zebrafish model facilitate tracking of fluorescent neutrophils in real time, in order to monitor inflammation, and assess the impact of therapeutic agents.

Original languageEnglish (US)
Pages (from-to)231-239
Number of pages9
JournalJournal of Inflammation Research
Volume12
DOIs
StatePublished - 2019

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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