Epithelial-to-mesenchymal transition confers pericyte properties on cancer cells

Anitha K. Shenoy, Yue Jin, Huacheng Luo, Ming Tang, Christine Pampo, Rong Shao, Dietmar W. Siemann, Lizi Wu, Coy D. Heldermon, Brian K. Law, Lung Ji Chang, Jianrong Lu

Research output: Contribution to journalArticle

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Abstract

Carcinoma cells can acquire increased motility and invasiveness through epithelial-to-mesenchymal transition (EMT). However, the significance of EMT in cancer metastasis has been controversial, and the exact fates and functions of EMT cancer cells in vivo remain inadequately understood. Here, we tracked epithelial cancer cells that underwent inducible or spontaneous EMT in various tumor transplantation models. Unlike epithelial cells, the majority of EMT cancer cells were specifically located in the perivascular space and closely associated with blood vessels. EMT markedly activated multiple pericyte markers in carcinoma cells, in particular PDGFR- and N-cadherin, which enabled EMT cells to be chemoattracted towards and physically interact with endothelium. In tumor xenografts generated from carcinoma cells that were prone to spontaneous EMT, a substantial fraction of the pericytes associated with tumor vasculature were derived from EMT cancer cells. Depletion of such EMT cells in transplanted tumors diminished pericyte coverage, impaired vascular integrity, and attenuated tumor growth. These findings suggest that EMT confers key pericyte attributes on cancer cells. The resulting EMT cells phenotypically and functionally resemble pericytes and are indispensable for vascular stabilization and sustained tumor growth. This study thus proposes a previously unrecognized role for EMT in cancer.

Original languageEnglish (US)
Pages (from-to)4174-4186
Number of pages13
JournalJournal of Clinical Investigation
Volume126
Issue number11
DOIs
StatePublished - Nov 1 2016

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Pericytes
Epithelial-Mesenchymal Transition
Neoplasms
Blood Vessels
Carcinoma
Epithelial Cells
Cadherins
Growth
Heterografts
Endothelium

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Shenoy, Anitha K. ; Jin, Yue ; Luo, Huacheng ; Tang, Ming ; Pampo, Christine ; Shao, Rong ; Siemann, Dietmar W. ; Wu, Lizi ; Heldermon, Coy D. ; Law, Brian K. ; Chang, Lung Ji ; Lu, Jianrong. / Epithelial-to-mesenchymal transition confers pericyte properties on cancer cells. In: Journal of Clinical Investigation. 2016 ; Vol. 126, No. 11. pp. 4174-4186.
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Shenoy, AK, Jin, Y, Luo, H, Tang, M, Pampo, C, Shao, R, Siemann, DW, Wu, L, Heldermon, CD, Law, BK, Chang, LJ & Lu, J 2016, 'Epithelial-to-mesenchymal transition confers pericyte properties on cancer cells', Journal of Clinical Investigation, vol. 126, no. 11, pp. 4174-4186. https://doi.org/10.1172/JCI86623

Epithelial-to-mesenchymal transition confers pericyte properties on cancer cells. / Shenoy, Anitha K.; Jin, Yue; Luo, Huacheng; Tang, Ming; Pampo, Christine; Shao, Rong; Siemann, Dietmar W.; Wu, Lizi; Heldermon, Coy D.; Law, Brian K.; Chang, Lung Ji; Lu, Jianrong.

In: Journal of Clinical Investigation, Vol. 126, No. 11, 01.11.2016, p. 4174-4186.

Research output: Contribution to journalArticle

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AU - Chang, Lung Ji

AU - Lu, Jianrong

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