Epoxide forming and degrading enzymes in the spider mite, Tetranychus urticae

Christopher Albert Mullin, F. Matsumura, B. A. Croft

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

1. Enzymes associated with the epoxidation and epoxide hydration or glutathione conjugation pathway occurred in the herbivorous mite, Tetranychus urticae. 2. Epoxidation of aldrin was primarily microsomal, required NADPH, was associated with a NADPH-cytochrome c reductase, and was inhibited by CO, 1-phenylimidazole and piperonyl butoxide. 3. Trans- and cis-epoxide hydrolases resided mostly in the microsomal fraction but were localized also in the cytosol. These activities were differentially inhibited by l,2-epoxy-3,3,3-trichloropropane, and chalcone and 4-phenylchalcone oxides. 4. In vitro and in vivo rates of aldrin epoxidation were very similar indicating that in vitro artifacts were not impairing full enzyme measurement. This was further confirmed in experiments with enzyme stabilizers.

Original languageEnglish (US)
Pages (from-to)85-92
Number of pages8
JournalComparative Biochemistry and Physiology. Part C, Comparative
Volume79
Issue number1
DOIs
StatePublished - Jan 1 1984

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Tetranychidae
Epoxy Compounds
Aldrin
Enzymes
Piperonyl Butoxide
Chalcone
Epoxide Hydrolases
NADPH-Ferrihemoprotein Reductase
Mites
Carbon Monoxide
NADP
Cytosol
Artifacts
Glutathione
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Immunology
  • Pharmacology

Cite this

Mullin, Christopher Albert ; Matsumura, F. ; Croft, B. A. / Epoxide forming and degrading enzymes in the spider mite, Tetranychus urticae. In: Comparative Biochemistry and Physiology. Part C, Comparative. 1984 ; Vol. 79, No. 1. pp. 85-92.
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Epoxide forming and degrading enzymes in the spider mite, Tetranychus urticae. / Mullin, Christopher Albert; Matsumura, F.; Croft, B. A.

In: Comparative Biochemistry and Physiology. Part C, Comparative, Vol. 79, No. 1, 01.01.1984, p. 85-92.

Research output: Contribution to journalArticle

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