Error in the calibration of the mgatp chemical‐shift limit: Effects on the determination of free magnesium by 31P NMR spectroscopy

Timothy Mosher, Gerald D. Williams, Chris Doumen, Kathryn F. Lanoue, Michael B. Smith

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The measurement of free intracellular magnesium (Mg2+) using the 31P chemical shifts of ATP requires the use of appropriate calibration solutions to determine the chemical‐shift limits ΔATPαβ and ΔMgATPαβ. Solutions containing excess Mg2+ contain significant amounts of Mg2ATP and yield positive errors in the value of ΔMgATPαβ. For physiological applications this may overestimate free intracellular Mg2+ by as much as 300%. This error may be minimized if appropriate mole ratios of Mg2+/ATP are used to calibrate ΔMgATPαβ. © 1992 Academic Press, Inc.

Original languageEnglish (US)
Pages (from-to)163-169
Number of pages7
JournalMagnetic Resonance in Medicine
Volume24
Issue number1
DOIs
StatePublished - Jan 1 1992

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Magnesium
Calibration
Magnetic Resonance Spectroscopy
Adenosine Triphosphate

All Science Journal Classification (ASJC) codes

  • Radiology Nuclear Medicine and imaging

Cite this

Mosher, Timothy ; Williams, Gerald D. ; Doumen, Chris ; Lanoue, Kathryn F. ; Smith, Michael B. / Error in the calibration of the mgatp chemical‐shift limit : Effects on the determination of free magnesium by 31P NMR spectroscopy. In: Magnetic Resonance in Medicine. 1992 ; Vol. 24, No. 1. pp. 163-169.
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Error in the calibration of the mgatp chemical‐shift limit : Effects on the determination of free magnesium by 31P NMR spectroscopy. / Mosher, Timothy; Williams, Gerald D.; Doumen, Chris; Lanoue, Kathryn F.; Smith, Michael B.

In: Magnetic Resonance in Medicine, Vol. 24, No. 1, 01.01.1992, p. 163-169.

Research output: Contribution to journalArticle

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