Abstract
Introduction: Erythropoietin (EPO) has been identified as a neuroregenerative agent. We hypothesize that it may accelerate recovery after crush injury and may vary with crush severity. Methods: Mice were randomized to mild, moderate, or severe crush of the sciatic nerve and were treated with EPO or vehicle control after injury. The sciatic function index (SFI) was monitored over the first week. Microstructural changes were analyzed by immunofluorescence for neurofilament (NF) and myelin (P0), and electron microscopy was used to assess ultrastructural changes. Results: In moderate crush injuries, EPO significantly improved SFI at 7 days post-injury, an effect not observed with other severity levels. Increases in the ratio of P0 to NF were observed after EPO treatment in moderate crush injuries. Electron microscopy demonstrated endothelial cell hypertrophy in the EPO group. Conclusions: EPO accelerates recovery in moderately crushed nerves, which may be through effects on myelination and vascularization. Injury severity may influence the efficacy of EPO. Muscle Nerve 56: 143–151, 2017.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 143-151 |
| Number of pages | 9 |
| Journal | Muscle and Nerve |
| Volume | 56 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 1 2017 |
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All Science Journal Classification (ASJC) codes
- Physiology
- Clinical Neurology
- Cellular and Molecular Neuroscience
- Physiology (medical)
Cite this
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Erythropoietin accelerates functional recovery after moderate sciatic nerve crush injury. / Geary, Michael B.; Li, Haiyan; Zingman, Alissa; Ketz, John; Zuscik, Michael; De Mesy Bentley, Karen L.; Noble, Mark; Elfar, John.
In: Muscle and Nerve, Vol. 56, No. 1, 01.07.2017, p. 143-151.Research output: Contribution to journal › Article
TY - JOUR
T1 - Erythropoietin accelerates functional recovery after moderate sciatic nerve crush injury
AU - Geary, Michael B.
AU - Li, Haiyan
AU - Zingman, Alissa
AU - Ketz, John
AU - Zuscik, Michael
AU - De Mesy Bentley, Karen L.
AU - Noble, Mark
AU - Elfar, John
PY - 2017/7/1
Y1 - 2017/7/1
N2 - Introduction: Erythropoietin (EPO) has been identified as a neuroregenerative agent. We hypothesize that it may accelerate recovery after crush injury and may vary with crush severity. Methods: Mice were randomized to mild, moderate, or severe crush of the sciatic nerve and were treated with EPO or vehicle control after injury. The sciatic function index (SFI) was monitored over the first week. Microstructural changes were analyzed by immunofluorescence for neurofilament (NF) and myelin (P0), and electron microscopy was used to assess ultrastructural changes. Results: In moderate crush injuries, EPO significantly improved SFI at 7 days post-injury, an effect not observed with other severity levels. Increases in the ratio of P0 to NF were observed after EPO treatment in moderate crush injuries. Electron microscopy demonstrated endothelial cell hypertrophy in the EPO group. Conclusions: EPO accelerates recovery in moderately crushed nerves, which may be through effects on myelination and vascularization. Injury severity may influence the efficacy of EPO. Muscle Nerve 56: 143–151, 2017.
AB - Introduction: Erythropoietin (EPO) has been identified as a neuroregenerative agent. We hypothesize that it may accelerate recovery after crush injury and may vary with crush severity. Methods: Mice were randomized to mild, moderate, or severe crush of the sciatic nerve and were treated with EPO or vehicle control after injury. The sciatic function index (SFI) was monitored over the first week. Microstructural changes were analyzed by immunofluorescence for neurofilament (NF) and myelin (P0), and electron microscopy was used to assess ultrastructural changes. Results: In moderate crush injuries, EPO significantly improved SFI at 7 days post-injury, an effect not observed with other severity levels. Increases in the ratio of P0 to NF were observed after EPO treatment in moderate crush injuries. Electron microscopy demonstrated endothelial cell hypertrophy in the EPO group. Conclusions: EPO accelerates recovery in moderately crushed nerves, which may be through effects on myelination and vascularization. Injury severity may influence the efficacy of EPO. Muscle Nerve 56: 143–151, 2017.
UR - http://www.scopus.com/inward/record.url?scp=85013066294&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85013066294&partnerID=8YFLogxK
U2 - 10.1002/mus.25459
DO - 10.1002/mus.25459
M3 - Article
C2 - 28168703
AN - SCOPUS:85013066294
VL - 56
SP - 143
EP - 151
JO - Muscle and Nerve
JF - Muscle and Nerve
SN - 0148-639X
IS - 1
ER -