Erythropoietin accelerates functional recovery after moderate sciatic nerve crush injury

Michael B. Geary, Haiyan Li, Alissa Zingman, John Ketz, Michael Zuscik, Karen L. De Mesy Bentley, Mark Noble, John Elfar

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Introduction: Erythropoietin (EPO) has been identified as a neuroregenerative agent. We hypothesize that it may accelerate recovery after crush injury and may vary with crush severity. Methods: Mice were randomized to mild, moderate, or severe crush of the sciatic nerve and were treated with EPO or vehicle control after injury. The sciatic function index (SFI) was monitored over the first week. Microstructural changes were analyzed by immunofluorescence for neurofilament (NF) and myelin (P0), and electron microscopy was used to assess ultrastructural changes. Results: In moderate crush injuries, EPO significantly improved SFI at 7 days post-injury, an effect not observed with other severity levels. Increases in the ratio of P0 to NF were observed after EPO treatment in moderate crush injuries. Electron microscopy demonstrated endothelial cell hypertrophy in the EPO group. Conclusions: EPO accelerates recovery in moderately crushed nerves, which may be through effects on myelination and vascularization. Injury severity may influence the efficacy of EPO. Muscle Nerve 56: 143–151, 2017.

Original languageEnglish (US)
Pages (from-to)143-151
Number of pages9
JournalMuscle and Nerve
Volume56
Issue number1
DOIs
StatePublished - Jul 1 2017

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Nerve Crush
Sciatic Nerve
Erythropoietin
Intermediate Filaments
Electron Microscopy
Wounds and Injuries
Myelin Sheath
Crush Injuries
Hypertrophy
Fluorescent Antibody Technique
Endothelial Cells
Muscles

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)

Cite this

Geary, M. B., Li, H., Zingman, A., Ketz, J., Zuscik, M., De Mesy Bentley, K. L., ... Elfar, J. (2017). Erythropoietin accelerates functional recovery after moderate sciatic nerve crush injury. Muscle and Nerve, 56(1), 143-151. https://doi.org/10.1002/mus.25459
Geary, Michael B. ; Li, Haiyan ; Zingman, Alissa ; Ketz, John ; Zuscik, Michael ; De Mesy Bentley, Karen L. ; Noble, Mark ; Elfar, John. / Erythropoietin accelerates functional recovery after moderate sciatic nerve crush injury. In: Muscle and Nerve. 2017 ; Vol. 56, No. 1. pp. 143-151.
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Geary, MB, Li, H, Zingman, A, Ketz, J, Zuscik, M, De Mesy Bentley, KL, Noble, M & Elfar, J 2017, 'Erythropoietin accelerates functional recovery after moderate sciatic nerve crush injury', Muscle and Nerve, vol. 56, no. 1, pp. 143-151. https://doi.org/10.1002/mus.25459

Erythropoietin accelerates functional recovery after moderate sciatic nerve crush injury. / Geary, Michael B.; Li, Haiyan; Zingman, Alissa; Ketz, John; Zuscik, Michael; De Mesy Bentley, Karen L.; Noble, Mark; Elfar, John.

In: Muscle and Nerve, Vol. 56, No. 1, 01.07.2017, p. 143-151.

Research output: Contribution to journalArticle

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N2 - Introduction: Erythropoietin (EPO) has been identified as a neuroregenerative agent. We hypothesize that it may accelerate recovery after crush injury and may vary with crush severity. Methods: Mice were randomized to mild, moderate, or severe crush of the sciatic nerve and were treated with EPO or vehicle control after injury. The sciatic function index (SFI) was monitored over the first week. Microstructural changes were analyzed by immunofluorescence for neurofilament (NF) and myelin (P0), and electron microscopy was used to assess ultrastructural changes. Results: In moderate crush injuries, EPO significantly improved SFI at 7 days post-injury, an effect not observed with other severity levels. Increases in the ratio of P0 to NF were observed after EPO treatment in moderate crush injuries. Electron microscopy demonstrated endothelial cell hypertrophy in the EPO group. Conclusions: EPO accelerates recovery in moderately crushed nerves, which may be through effects on myelination and vascularization. Injury severity may influence the efficacy of EPO. Muscle Nerve 56: 143–151, 2017.

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Geary MB, Li H, Zingman A, Ketz J, Zuscik M, De Mesy Bentley KL et al. Erythropoietin accelerates functional recovery after moderate sciatic nerve crush injury. Muscle and Nerve. 2017 Jul 1;56(1):143-151. https://doi.org/10.1002/mus.25459