Erythropoietin stimulates a rise in intracellular free calcium concentration in single early human erythroid precursors

B. A. Miller, R. C. Scaduto, D. L. Tillotson, J. J. Botti, J. Y. Cheung

Research output: Contribution to journalArticle

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Abstract

Erythropoietin and granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulate the differentiation and proliferation of erythroid cells. To determine the cellular mechanism of action of these growth factors, we measured changes in intracellular free calcium concentration ([Ca(c)]) in single human erythroid precursors in response to recombinant erythropoietin and GM-CSF. [Ca(c)] in immature erythroblasts derived from cultured human cord blood erythroid progenitors was measured with fluorescence microscopy digital video imaging. When stimulated with erythropoietin, [Ca(c)] in the majority of erythroblasts increased within 3 min, peaked at 5 min, and returned toward baseline at 10 min. The percentage of cells that responded to erythropoietin stimulation increased in a dose-dependent manner. Additional stimulation with GM-CSF in cells previously exposed to erythropoietin resulted in a second [Ca(c)] increase. Immature erythroblasts treated with GM-CSF followed by erythropoietin responded similarly to each factor with a rise in [Ca(c)]. The source of transient calcium is intracellular since erythroblasts were incubated in medium devoid of extracellular calcium. Our observations suggest that changes in [Ca(c)] may be an intracellular signal that mediates the proliferative/differentiating effect of hematopoietic growth factors.

Original languageEnglish (US)
Pages (from-to)309-315
Number of pages7
JournalJournal of Clinical Investigation
Volume82
Issue number1
DOIs
StatePublished - Jan 1 1988

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Erythropoietin
Calcium
Erythroblasts
Granulocyte-Macrophage Colony-Stimulating Factor
Intercellular Signaling Peptides and Proteins
Erythroid Cells
Fetal Blood
Fluorescence Microscopy

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Miller, B. A. ; Scaduto, R. C. ; Tillotson, D. L. ; Botti, J. J. ; Cheung, J. Y. / Erythropoietin stimulates a rise in intracellular free calcium concentration in single early human erythroid precursors. In: Journal of Clinical Investigation. 1988 ; Vol. 82, No. 1. pp. 309-315.
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Erythropoietin stimulates a rise in intracellular free calcium concentration in single early human erythroid precursors. / Miller, B. A.; Scaduto, R. C.; Tillotson, D. L.; Botti, J. J.; Cheung, J. Y.

In: Journal of Clinical Investigation, Vol. 82, No. 1, 01.01.1988, p. 309-315.

Research output: Contribution to journalArticle

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