Escherichia coli O-antigen gene clusters of serogroups O62, O68, O131, O140, O142, and O163

DNA sequences and similarity between O62 and O68, and PCR-based serogrouping

Yanhong Liu, Xianghe Yan, Chitrita Debroy, Pina M. Fratamico, David S. Needleman, Robert W. Li, Wei Wang, Liliana Losada, Lauren Brinkac, Diana Radune, Magaly Toro, Narasimha Hegde, Jianghong Meng

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The DNA sequence of the O-antigen gene clusters of Escherichia coli serogroups O62, O68, O131, O140, O142, and O163 was determined, and primers based on the wzx (O-antigen flippase) and/or wzy (O-antigen polymerase) genes within the O-antigen gene clusters were designed and used in PCR assays to identify each serogroup. Specificity was tested with E. coli reference strains, field isolates belonging to the target serogroups, and non-E. coli bacteria. The PCR assays were highly specific for the respective serogroups; however, the PCR assay targeting the O62 wzx gene reacted positively with strains belonging to E. coli O68, which was determined by serotyping. Analysis of the O-antigen gene cluster sequences of serogroups O62 and O68 reference strains showed that they were 94% identical at the nucleotide level, although O62 contained an insertion sequence (IS) element located between the rmlA and rmlC genes within the O-antigen gene cluster. A PCR assay targeting the rmlA and rmlC genes flanking the IS element was used to differentiate O62 and O68 serogroups. The PCR assays developed in this study can be used for the detection and identification of E. coli O62/O68, O131, O140, O142, and O163 strains isolated from different sources.

Original languageEnglish (US)
Pages (from-to)51-68
Number of pages18
JournalBiosensors
Volume5
Issue number1
DOIs
StatePublished - Jan 1 2015

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O Antigens
DNA sequences
Multigene Family
Escherichia coli
Genes
Polymerase Chain Reaction
Assays
DNA Transposable Elements
Serotyping
Insertional Mutagenesis
Serogroup
Nucleotides
Bacteria

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

Liu, Yanhong ; Yan, Xianghe ; Debroy, Chitrita ; Fratamico, Pina M. ; Needleman, David S. ; Li, Robert W. ; Wang, Wei ; Losada, Liliana ; Brinkac, Lauren ; Radune, Diana ; Toro, Magaly ; Hegde, Narasimha ; Meng, Jianghong. / Escherichia coli O-antigen gene clusters of serogroups O62, O68, O131, O140, O142, and O163 : DNA sequences and similarity between O62 and O68, and PCR-based serogrouping. In: Biosensors. 2015 ; Vol. 5, No. 1. pp. 51-68.
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title = "Escherichia coli O-antigen gene clusters of serogroups O62, O68, O131, O140, O142, and O163: DNA sequences and similarity between O62 and O68, and PCR-based serogrouping",
abstract = "The DNA sequence of the O-antigen gene clusters of Escherichia coli serogroups O62, O68, O131, O140, O142, and O163 was determined, and primers based on the wzx (O-antigen flippase) and/or wzy (O-antigen polymerase) genes within the O-antigen gene clusters were designed and used in PCR assays to identify each serogroup. Specificity was tested with E. coli reference strains, field isolates belonging to the target serogroups, and non-E. coli bacteria. The PCR assays were highly specific for the respective serogroups; however, the PCR assay targeting the O62 wzx gene reacted positively with strains belonging to E. coli O68, which was determined by serotyping. Analysis of the O-antigen gene cluster sequences of serogroups O62 and O68 reference strains showed that they were 94{\%} identical at the nucleotide level, although O62 contained an insertion sequence (IS) element located between the rmlA and rmlC genes within the O-antigen gene cluster. A PCR assay targeting the rmlA and rmlC genes flanking the IS element was used to differentiate O62 and O68 serogroups. The PCR assays developed in this study can be used for the detection and identification of E. coli O62/O68, O131, O140, O142, and O163 strains isolated from different sources.",
author = "Yanhong Liu and Xianghe Yan and Chitrita Debroy and Fratamico, {Pina M.} and Needleman, {David S.} and Li, {Robert W.} and Wei Wang and Liliana Losada and Lauren Brinkac and Diana Radune and Magaly Toro and Narasimha Hegde and Jianghong Meng",
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Liu, Y, Yan, X, Debroy, C, Fratamico, PM, Needleman, DS, Li, RW, Wang, W, Losada, L, Brinkac, L, Radune, D, Toro, M, Hegde, N & Meng, J 2015, 'Escherichia coli O-antigen gene clusters of serogroups O62, O68, O131, O140, O142, and O163: DNA sequences and similarity between O62 and O68, and PCR-based serogrouping', Biosensors, vol. 5, no. 1, pp. 51-68. https://doi.org/10.3390/bios5010051

Escherichia coli O-antigen gene clusters of serogroups O62, O68, O131, O140, O142, and O163 : DNA sequences and similarity between O62 and O68, and PCR-based serogrouping. / Liu, Yanhong; Yan, Xianghe; Debroy, Chitrita; Fratamico, Pina M.; Needleman, David S.; Li, Robert W.; Wang, Wei; Losada, Liliana; Brinkac, Lauren; Radune, Diana; Toro, Magaly; Hegde, Narasimha; Meng, Jianghong.

In: Biosensors, Vol. 5, No. 1, 01.01.2015, p. 51-68.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Escherichia coli O-antigen gene clusters of serogroups O62, O68, O131, O140, O142, and O163

T2 - DNA sequences and similarity between O62 and O68, and PCR-based serogrouping

AU - Liu, Yanhong

AU - Yan, Xianghe

AU - Debroy, Chitrita

AU - Fratamico, Pina M.

AU - Needleman, David S.

AU - Li, Robert W.

AU - Wang, Wei

AU - Losada, Liliana

AU - Brinkac, Lauren

AU - Radune, Diana

AU - Toro, Magaly

AU - Hegde, Narasimha

AU - Meng, Jianghong

PY - 2015/1/1

Y1 - 2015/1/1

N2 - The DNA sequence of the O-antigen gene clusters of Escherichia coli serogroups O62, O68, O131, O140, O142, and O163 was determined, and primers based on the wzx (O-antigen flippase) and/or wzy (O-antigen polymerase) genes within the O-antigen gene clusters were designed and used in PCR assays to identify each serogroup. Specificity was tested with E. coli reference strains, field isolates belonging to the target serogroups, and non-E. coli bacteria. The PCR assays were highly specific for the respective serogroups; however, the PCR assay targeting the O62 wzx gene reacted positively with strains belonging to E. coli O68, which was determined by serotyping. Analysis of the O-antigen gene cluster sequences of serogroups O62 and O68 reference strains showed that they were 94% identical at the nucleotide level, although O62 contained an insertion sequence (IS) element located between the rmlA and rmlC genes within the O-antigen gene cluster. A PCR assay targeting the rmlA and rmlC genes flanking the IS element was used to differentiate O62 and O68 serogroups. The PCR assays developed in this study can be used for the detection and identification of E. coli O62/O68, O131, O140, O142, and O163 strains isolated from different sources.

AB - The DNA sequence of the O-antigen gene clusters of Escherichia coli serogroups O62, O68, O131, O140, O142, and O163 was determined, and primers based on the wzx (O-antigen flippase) and/or wzy (O-antigen polymerase) genes within the O-antigen gene clusters were designed and used in PCR assays to identify each serogroup. Specificity was tested with E. coli reference strains, field isolates belonging to the target serogroups, and non-E. coli bacteria. The PCR assays were highly specific for the respective serogroups; however, the PCR assay targeting the O62 wzx gene reacted positively with strains belonging to E. coli O68, which was determined by serotyping. Analysis of the O-antigen gene cluster sequences of serogroups O62 and O68 reference strains showed that they were 94% identical at the nucleotide level, although O62 contained an insertion sequence (IS) element located between the rmlA and rmlC genes within the O-antigen gene cluster. A PCR assay targeting the rmlA and rmlC genes flanking the IS element was used to differentiate O62 and O68 serogroups. The PCR assays developed in this study can be used for the detection and identification of E. coli O62/O68, O131, O140, O142, and O163 strains isolated from different sources.

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