Essential Oils Against Pathogen and Spoilage Microorganisms of Fruit Juices: Use of Versatile Antimicrobial Delivery Systems

Valentina Trinetta, Mark T. Morgan, John Neil Coupland, Umut Yucel

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Essential oils (EO) are increasingly used as natural antimicrobial compounds, however the effect of delivery system to enhance their antimicrobial activity has not been widely studied. Limonene (0 to 10 μL/mL) was added to microbial suspensions (∼105 CFU/mL) of selected foodborne pathogens (Listeria monocytogenes Scott A, Salmonella enterica Typhimurium, Escherichia coli and Staphylococcus aureus), and spoilage microorganisms (Lactobacillus plantarum, Saccharomyces cerevisiae, and Candida albicans). S. aureus was found to be the most sensitive foodborne pathogen while Salmonella enterica showed continued growth under all concentrations. Stable nanoemulsions and solid lipid nanoparticles (SLN) (d ∼ 170 nm) were prepared using an alkane carrier oil (n-tetradecane and n-eicosane, respectively). Interfacial effects and homogenous distribution of limonene in nanoemulsions improved its (8 and 12 μL/mL) antimicrobial effect against S. aureus. Higher aqueous concentrations as a result of expulsion from SLN further enhanced the antimicrobial activity pronounced at higher limonene concentrations. Therefore, our findings confirm that the emulsion-based delivery systems are able to effectively distribute limonene inside a microbial suspension to improve its antimicrobial activity.

Original languageEnglish (US)
Pages (from-to)471-476
Number of pages6
JournalJournal of Food Science
Volume82
Issue number2
DOIs
StatePublished - Feb 1 2017

Fingerprint

fruit juices
Volatile Oils
essential oils
anti-infective agents
nanoemulsions
Staphylococcus aureus
Salmonella enterica
pathogens
nanoparticles
food pathogens
Nanoparticles
Suspensions
Lipids
Lactobacillus plantarum
Alkanes
Listeria monocytogenes
anti-infective properties
Salmonella typhimurium
lipids
Emulsions

All Science Journal Classification (ASJC) codes

  • Food Science

Cite this

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abstract = "Essential oils (EO) are increasingly used as natural antimicrobial compounds, however the effect of delivery system to enhance their antimicrobial activity has not been widely studied. Limonene (0 to 10 μL/mL) was added to microbial suspensions (∼105 CFU/mL) of selected foodborne pathogens (Listeria monocytogenes Scott A, Salmonella enterica Typhimurium, Escherichia coli and Staphylococcus aureus), and spoilage microorganisms (Lactobacillus plantarum, Saccharomyces cerevisiae, and Candida albicans). S. aureus was found to be the most sensitive foodborne pathogen while Salmonella enterica showed continued growth under all concentrations. Stable nanoemulsions and solid lipid nanoparticles (SLN) (d ∼ 170 nm) were prepared using an alkane carrier oil (n-tetradecane and n-eicosane, respectively). Interfacial effects and homogenous distribution of limonene in nanoemulsions improved its (8 and 12 μL/mL) antimicrobial effect against S. aureus. Higher aqueous concentrations as a result of expulsion from SLN further enhanced the antimicrobial activity pronounced at higher limonene concentrations. Therefore, our findings confirm that the emulsion-based delivery systems are able to effectively distribute limonene inside a microbial suspension to improve its antimicrobial activity.",
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Essential Oils Against Pathogen and Spoilage Microorganisms of Fruit Juices : Use of Versatile Antimicrobial Delivery Systems. / Trinetta, Valentina; Morgan, Mark T.; Coupland, John Neil; Yucel, Umut.

In: Journal of Food Science, Vol. 82, No. 2, 01.02.2017, p. 471-476.

Research output: Contribution to journalArticle

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