Essential Role of NF-κB Activation in Silica-Induced Inflammatory Mediator Production in Macrophages

Fei Chen, Shao Cong Sun, Douglas C. Kuh, Lesley J. Gaydos, Laurence M. Demers

Research output: Contribution to journalArticle

94 Scopus citations

Abstract

In this report, we demonstrate that NF-κB, a ubiquitous transcription factor, plays an essential role in silica-induced inflammatory mediator production in the mouse macrophage cell line RAW 264.7. Compared to the effect of lipopolysaccharide (LPS), silica mediated a stronger activation of NF-κB p50/p50 homodimer at early phase of poststimulation. Furthermore, activation of NF-κB by silica and LPS appears to be mediated by different signal transduction pathways. Both silica and LPS increased mRNA expression in these cells for cyclooxygenase II, inducible nitric oxide synthase, tumor necrosis factor-α and interleukin-1α. This expression was attenuated along with the inhibition of NF-κB activation.

Original languageEnglish (US)
Pages (from-to)985-992
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume214
Issue number3
DOIs
StatePublished - Jan 1 1995

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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