Establishment and characterization of a new endometrial cancer cell line (SCRC-1)

J. Gorodecki, R. Mortel, R. L. Ladda, S. P. Ward, L. Geder, F. Rapp

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A new human endometrial cell line, SCRC-1, has been established from a uterine adenocarcinoma. During a period of 57 wk in culture, the SCRC-1 cells have been passaged over 100 times. Cultures have an epithelioid morphology, high mitotic activity, and a tendency to form multiple cell layers. The cells are polygonal and are arranged in pavement-like fashion. Most have multiple nucleoli, some are multinucleated, and all have heavily vacuolated cytoplasm. Pretreatment with iododeoxyuridine did not reveal viral markers by cocultivation or immunofluorescence techniques. Karyologic studies indicate that the SCRC-1 cell line is essentially diploid with a small subpopulation of tetraploid cells. Over a period of 20 wk, 1 of 14 athymic nude mice inoculated with SCRC-1 cells developed a tumor which histologically closely resembled the original human tumor. Cells were subsequently propagated from this tumor and the resulting epithelioid-like cell line was designated SCRC-1, Tu 1.

Original languageEnglish (US)
Pages (from-to)671-679
Number of pages9
JournalAmerican journal of obstetrics and gynecology
Volume135
Issue number5
DOIs
StatePublished - Jan 1 1979

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Endometrial Neoplasms
Cell Line
Nude Mice
Idoxuridine
Epithelioid Cells
Neoplasms
Tetraploidy
Coculture Techniques
Diploidy
Fluorescent Antibody Technique
Cytoplasm
Adenocarcinoma
Biomarkers

All Science Journal Classification (ASJC) codes

  • Obstetrics and Gynecology

Cite this

Gorodecki, J. ; Mortel, R. ; Ladda, R. L. ; Ward, S. P. ; Geder, L. ; Rapp, F. / Establishment and characterization of a new endometrial cancer cell line (SCRC-1). In: American journal of obstetrics and gynecology. 1979 ; Vol. 135, No. 5. pp. 671-679.
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Establishment and characterization of a new endometrial cancer cell line (SCRC-1). / Gorodecki, J.; Mortel, R.; Ladda, R. L.; Ward, S. P.; Geder, L.; Rapp, F.

In: American journal of obstetrics and gynecology, Vol. 135, No. 5, 01.01.1979, p. 671-679.

Research output: Contribution to journalArticle

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