Establishment of a human hepatocyte line that supports in vitro development of the exo-erythrocytic stages of the malaria parasites Plasmodium falciparum and P. vivax

Jetsumon Sattabongkot, Nongnuch Yimamnuaychoke, Surasak Leelaudomlipi, Maneerat Rasameesoraj, Rachaneeporn Jenwithisuk, Russell E. Coleman, Rachanee Udomsangpetch, Liwang Cui, Thomas G. Brewer

Research output: Contribution to journalArticle

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Abstract

Our understanding of the biology of malaria parasite liver stages is limited because of the lack of efficient in vitro systems that support the exo-erythrocytic (EE) development of the parasite. We report the development of a new hepatocyte line (HC-04) from normal human liver cells. The HC-04 cells have proliferated in hormone-free medium for more than 200 passages. The cells were hyperdiploid, resembled liver parenchymal cells, and synthesized major liverspecific proteins and enzymes. Using Plasmodium falciparum and P. vivax sporozoites harvested from salivary glands of infected mosquitoes, we showed that HC-04 cells supported the complete EE development of these two most prevalent human malaria parasites. The EE parasites attained full maturation as shown by their infectivity to human erythrocytes. The infection rates of the liver cells were estimated to be 0.066% and 0.041%for P. falciparum and P. vivax, respectively. As the first human hepatocyte line known to support complete EE development of both P. falciparum and P. vivax, HC-04 will provide an experimental model that can be used for studying the biology of liver stage malaria parasites.

Original languageEnglish (US)
Pages (from-to)708-715
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume74
Issue number5
StatePublished - May 1 2006

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Falciparum Malaria
Hepatocytes
Parasites
Plasmodium falciparum
Liver
Malaria
Sporozoites
Polyploidy
Salivary Glands
Culicidae
In Vitro Techniques
Theoretical Models
Erythrocytes
Hormones
Enzymes
Infection
Proteins

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

Sattabongkot, J., Yimamnuaychoke, N., Leelaudomlipi, S., Rasameesoraj, M., Jenwithisuk, R., Coleman, R. E., ... Brewer, T. G. (2006). Establishment of a human hepatocyte line that supports in vitro development of the exo-erythrocytic stages of the malaria parasites Plasmodium falciparum and P. vivax. American Journal of Tropical Medicine and Hygiene, 74(5), 708-715.
Sattabongkot, Jetsumon ; Yimamnuaychoke, Nongnuch ; Leelaudomlipi, Surasak ; Rasameesoraj, Maneerat ; Jenwithisuk, Rachaneeporn ; Coleman, Russell E. ; Udomsangpetch, Rachanee ; Cui, Liwang ; Brewer, Thomas G. / Establishment of a human hepatocyte line that supports in vitro development of the exo-erythrocytic stages of the malaria parasites Plasmodium falciparum and P. vivax. In: American Journal of Tropical Medicine and Hygiene. 2006 ; Vol. 74, No. 5. pp. 708-715.
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abstract = "Our understanding of the biology of malaria parasite liver stages is limited because of the lack of efficient in vitro systems that support the exo-erythrocytic (EE) development of the parasite. We report the development of a new hepatocyte line (HC-04) from normal human liver cells. The HC-04 cells have proliferated in hormone-free medium for more than 200 passages. The cells were hyperdiploid, resembled liver parenchymal cells, and synthesized major liverspecific proteins and enzymes. Using Plasmodium falciparum and P. vivax sporozoites harvested from salivary glands of infected mosquitoes, we showed that HC-04 cells supported the complete EE development of these two most prevalent human malaria parasites. The EE parasites attained full maturation as shown by their infectivity to human erythrocytes. The infection rates of the liver cells were estimated to be 0.066{\%} and 0.041{\%}for P. falciparum and P. vivax, respectively. As the first human hepatocyte line known to support complete EE development of both P. falciparum and P. vivax, HC-04 will provide an experimental model that can be used for studying the biology of liver stage malaria parasites.",
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Sattabongkot, J, Yimamnuaychoke, N, Leelaudomlipi, S, Rasameesoraj, M, Jenwithisuk, R, Coleman, RE, Udomsangpetch, R, Cui, L & Brewer, TG 2006, 'Establishment of a human hepatocyte line that supports in vitro development of the exo-erythrocytic stages of the malaria parasites Plasmodium falciparum and P. vivax', American Journal of Tropical Medicine and Hygiene, vol. 74, no. 5, pp. 708-715.

Establishment of a human hepatocyte line that supports in vitro development of the exo-erythrocytic stages of the malaria parasites Plasmodium falciparum and P. vivax. / Sattabongkot, Jetsumon; Yimamnuaychoke, Nongnuch; Leelaudomlipi, Surasak; Rasameesoraj, Maneerat; Jenwithisuk, Rachaneeporn; Coleman, Russell E.; Udomsangpetch, Rachanee; Cui, Liwang; Brewer, Thomas G.

In: American Journal of Tropical Medicine and Hygiene, Vol. 74, No. 5, 01.05.2006, p. 708-715.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Establishment of a human hepatocyte line that supports in vitro development of the exo-erythrocytic stages of the malaria parasites Plasmodium falciparum and P. vivax

AU - Sattabongkot, Jetsumon

AU - Yimamnuaychoke, Nongnuch

AU - Leelaudomlipi, Surasak

AU - Rasameesoraj, Maneerat

AU - Jenwithisuk, Rachaneeporn

AU - Coleman, Russell E.

AU - Udomsangpetch, Rachanee

AU - Cui, Liwang

AU - Brewer, Thomas G.

PY - 2006/5/1

Y1 - 2006/5/1

N2 - Our understanding of the biology of malaria parasite liver stages is limited because of the lack of efficient in vitro systems that support the exo-erythrocytic (EE) development of the parasite. We report the development of a new hepatocyte line (HC-04) from normal human liver cells. The HC-04 cells have proliferated in hormone-free medium for more than 200 passages. The cells were hyperdiploid, resembled liver parenchymal cells, and synthesized major liverspecific proteins and enzymes. Using Plasmodium falciparum and P. vivax sporozoites harvested from salivary glands of infected mosquitoes, we showed that HC-04 cells supported the complete EE development of these two most prevalent human malaria parasites. The EE parasites attained full maturation as shown by their infectivity to human erythrocytes. The infection rates of the liver cells were estimated to be 0.066% and 0.041%for P. falciparum and P. vivax, respectively. As the first human hepatocyte line known to support complete EE development of both P. falciparum and P. vivax, HC-04 will provide an experimental model that can be used for studying the biology of liver stage malaria parasites.

AB - Our understanding of the biology of malaria parasite liver stages is limited because of the lack of efficient in vitro systems that support the exo-erythrocytic (EE) development of the parasite. We report the development of a new hepatocyte line (HC-04) from normal human liver cells. The HC-04 cells have proliferated in hormone-free medium for more than 200 passages. The cells were hyperdiploid, resembled liver parenchymal cells, and synthesized major liverspecific proteins and enzymes. Using Plasmodium falciparum and P. vivax sporozoites harvested from salivary glands of infected mosquitoes, we showed that HC-04 cells supported the complete EE development of these two most prevalent human malaria parasites. The EE parasites attained full maturation as shown by their infectivity to human erythrocytes. The infection rates of the liver cells were estimated to be 0.066% and 0.041%for P. falciparum and P. vivax, respectively. As the first human hepatocyte line known to support complete EE development of both P. falciparum and P. vivax, HC-04 will provide an experimental model that can be used for studying the biology of liver stage malaria parasites.

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