Establishment of fast-growing callus and root cultures of Cephalotaxus harringtonia

Enaksha R. Wickremesinhe, Richard N. Arteca

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Callus cultures were established from Cephalotaxus harringtonia (Japanese plumyew) stem expiants cultured on Murashige and Skoog medium supplemented with 4.5 μM 2,4-dichlorophenoxyacetic acid and 0.05 βM 6-furfurylaminopurine. The inclusion of 4.9 μM 6-(γ,γ-dimethylallylamino) purine as the sole hormone significantly increased the growth rate of the callus. Organogenesis giving rise to both shoots and roots occurred upon transfer of the callus onto a hormonefree medium. Vitrification was common on all regenerated shoots cultured on Gelrite-containing medium. Regenerated roots were excised and established in McCown's woody plant medium. Doubling the phosphate and nitrate levels in the medium increased the growth of these root cultures.

Original languageEnglish (US)
Pages (from-to)80-83
Number of pages4
JournalPlant Cell Reports
Volume12
Issue number2
DOIs
StatePublished - Jan 1 1993

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

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