Establishment of polarity during organization of the acentrosomal plant cortical microtubule array

Ram Dixit, Eric Chang, Richard Cyr

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

The plant cortical microtubule array is a unique acentrosomal array that is essential for plant morphogenesis. To understand how this array is organized, we exploited the microtubule (+)-end tracking activity of two Arabidopsis EB1 proteins in combination with FRAP (fluorescence recovery after photobleaching) experiments of GFP-tubulin to examine the relationship between cortical microtubule array organization and polarity. Significantly, our observations show that the majority of cortical microtubules in ordered arrays, within a particular cell, face the same direction in both Arabidopsis plants and cultured tobacco cells. We determined that this polar microtubule coalignment is at least partially due to a selective stabilization of microtubules, and not due to a change in microtubule polymerization rates. Finally, we show that polar microtubule coalignment occurs in conjunction with parallel grouping of cortical microtubules and that cortical array polarity is progressively enhanced during array organization. These observations reveal a novel aspect of plant cortical microtubule array organization and suggest that selective stabilization of dynamic cortical microtubules plays a predominant role in the self-organization of cortical arrays.

Original languageEnglish (US)
Pages (from-to)1298-1305
Number of pages8
JournalMolecular biology of the cell
Volume17
Issue number3
DOIs
StatePublished - Mar 1 2006

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Microtubules
Fluorescence Recovery After Photobleaching
Plant Development
Tubulin
Arabidopsis
Polymerization
Tobacco
Cultured Cells

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Dixit, Ram ; Chang, Eric ; Cyr, Richard. / Establishment of polarity during organization of the acentrosomal plant cortical microtubule array. In: Molecular biology of the cell. 2006 ; Vol. 17, No. 3. pp. 1298-1305.
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Establishment of polarity during organization of the acentrosomal plant cortical microtubule array. / Dixit, Ram; Chang, Eric; Cyr, Richard.

In: Molecular biology of the cell, Vol. 17, No. 3, 01.03.2006, p. 1298-1305.

Research output: Contribution to journalArticle

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