Estimation of Free Dopamine in the Cytoplasm of the Giant Dopamine Cell of Planorbis corneus by Voltammetry and Capillary Electrophoresis

Jennifer B. Chien, Ross A. Wallingford, Andrew G. Ewing

Research output: Contribution to journalArticlepeer-review

Abstract

Voltammetric electrodes having a tip diameter of 2–12 μ and microscale capillary zone electrophoresis have been used for dynamic and static monitoring of dopamine in the somal cytoplasm of the giant dopamine neuron of Planorbis corneus. Intracellular dopamine levels can be altered by extracellular application of dopamine or ethanol. The latter experiment provides a means to estimate total stores of endogenous dopamine, and the evidence presented suggests that at least 98% of these stores are bound and not directly accessible to the cytoplasm.

Original languageEnglish (US)
Pages (from-to)633-638
Number of pages6
JournalJournal of neurochemistry
Volume54
Issue number2
DOIs
StatePublished - Feb 1990

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

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