Estrogen and telomerase in human peripheral blood mononuclear cells

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The enzyme telomerase plays an important role in sustaining the capacity of T lymphocytes for homeostatic replication. Recent data have suggested that gonadal steroids might modulate telomerase expression or activity within these cells. We used quantitative assay techniques for both telomerase mRNA expression and telomerase enzymatic activity to systematically examine the effects of physiologic concentrations of estradiol on human peripheral blood mononuclear cells under basal conditions and under conditions that normally enhance telomerase activity in T lymphocytes. Cells from women tended to exhibit higher responsiveness of telomerase activity to induction by T cell receptor engagement. However, we found no evidence of a direct effect of physiologic concentrations of estradiol on human telomerase reverse transcriptase (hTERT) mRNA expression, hTERT protein expression, or telomerase enzymatic activity in cultured PBMCs. While estrogen might exert developmental effects on T cells to alter telomerase responsiveness to T cell receptor engagement, mature peripheral T cells do not respond to estradiol with changes in expression or function of telomerase.

Original languageEnglish (US)
Pages (from-to)83-88
Number of pages6
JournalMolecular and Cellular Endocrinology
Volume364
Issue number1-2
DOIs
StatePublished - Nov 25 2012

Fingerprint

Telomerase
Blood Cells
Estrogens
Blood
T-cells
T-Lymphocytes
Estradiol
T-Cell Antigen Receptor
Messenger RNA
Assays
Steroids
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

@article{bad260c565fc4ebb8cb09150056f27f1,
title = "Estrogen and telomerase in human peripheral blood mononuclear cells",
abstract = "The enzyme telomerase plays an important role in sustaining the capacity of T lymphocytes for homeostatic replication. Recent data have suggested that gonadal steroids might modulate telomerase expression or activity within these cells. We used quantitative assay techniques for both telomerase mRNA expression and telomerase enzymatic activity to systematically examine the effects of physiologic concentrations of estradiol on human peripheral blood mononuclear cells under basal conditions and under conditions that normally enhance telomerase activity in T lymphocytes. Cells from women tended to exhibit higher responsiveness of telomerase activity to induction by T cell receptor engagement. However, we found no evidence of a direct effect of physiologic concentrations of estradiol on human telomerase reverse transcriptase (hTERT) mRNA expression, hTERT protein expression, or telomerase enzymatic activity in cultured PBMCs. While estrogen might exert developmental effects on T cells to alter telomerase responsiveness to T cell receptor engagement, mature peripheral T cells do not respond to estradiol with changes in expression or function of telomerase.",
author = "Benko, {Ann L.} and Nancy Olsen and William Kovacs",
year = "2012",
month = "11",
day = "25",
doi = "10.1016/j.mce.2012.08.012",
language = "English (US)",
volume = "364",
pages = "83--88",
journal = "Molecular and Cellular Endocrinology",
issn = "0303-7207",
publisher = "Elsevier Ireland Ltd",
number = "1-2",

}

Estrogen and telomerase in human peripheral blood mononuclear cells. / Benko, Ann L.; Olsen, Nancy; Kovacs, William.

In: Molecular and Cellular Endocrinology, Vol. 364, No. 1-2, 25.11.2012, p. 83-88.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Estrogen and telomerase in human peripheral blood mononuclear cells

AU - Benko, Ann L.

AU - Olsen, Nancy

AU - Kovacs, William

PY - 2012/11/25

Y1 - 2012/11/25

N2 - The enzyme telomerase plays an important role in sustaining the capacity of T lymphocytes for homeostatic replication. Recent data have suggested that gonadal steroids might modulate telomerase expression or activity within these cells. We used quantitative assay techniques for both telomerase mRNA expression and telomerase enzymatic activity to systematically examine the effects of physiologic concentrations of estradiol on human peripheral blood mononuclear cells under basal conditions and under conditions that normally enhance telomerase activity in T lymphocytes. Cells from women tended to exhibit higher responsiveness of telomerase activity to induction by T cell receptor engagement. However, we found no evidence of a direct effect of physiologic concentrations of estradiol on human telomerase reverse transcriptase (hTERT) mRNA expression, hTERT protein expression, or telomerase enzymatic activity in cultured PBMCs. While estrogen might exert developmental effects on T cells to alter telomerase responsiveness to T cell receptor engagement, mature peripheral T cells do not respond to estradiol with changes in expression or function of telomerase.

AB - The enzyme telomerase plays an important role in sustaining the capacity of T lymphocytes for homeostatic replication. Recent data have suggested that gonadal steroids might modulate telomerase expression or activity within these cells. We used quantitative assay techniques for both telomerase mRNA expression and telomerase enzymatic activity to systematically examine the effects of physiologic concentrations of estradiol on human peripheral blood mononuclear cells under basal conditions and under conditions that normally enhance telomerase activity in T lymphocytes. Cells from women tended to exhibit higher responsiveness of telomerase activity to induction by T cell receptor engagement. However, we found no evidence of a direct effect of physiologic concentrations of estradiol on human telomerase reverse transcriptase (hTERT) mRNA expression, hTERT protein expression, or telomerase enzymatic activity in cultured PBMCs. While estrogen might exert developmental effects on T cells to alter telomerase responsiveness to T cell receptor engagement, mature peripheral T cells do not respond to estradiol with changes in expression or function of telomerase.

UR - http://www.scopus.com/inward/record.url?scp=84867554274&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84867554274&partnerID=8YFLogxK

U2 - 10.1016/j.mce.2012.08.012

DO - 10.1016/j.mce.2012.08.012

M3 - Article

C2 - 22954679

AN - SCOPUS:84867554274

VL - 364

SP - 83

EP - 88

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

SN - 0303-7207

IS - 1-2

ER -