Ethanol production from carob extract by using Saccharomyces cerevisiae in biofilm reactor

Mustafa Germec, Irfan Turhan, Mustafa Karhan, Ali Demirci

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Carob, widely grown in Mediterranean countries, contains several types of sugars such as 34 42% sucrose, 7-10% glucose, and 10-12% fructose. Due to the high sugar concentration, it is used for production of many value added products via fermentation such as ethanol, lactic acid, citric acid, pullulan, and mannanase. In this study, ethanol production conditions from carob extract through repeated-batch fermentation by using Saccharomycess cerevisiae in biofilm reactor were optimized by response surface methodology. Initial sugar concentration (4-10°Bx), agitation speed (100-200 rpm), and pH (5.0-6.0) were used as variable parameters. Results showed that initial sugar concentration had a significant effect on yield and maximum production rate. Optimum conditions were found to be 7.71°Bx, 120 rpm, and 5.18, respectively. After validation experiments ethanol concentration, yield, maximum production rate, and sugar utilization yield were determined as 24.51 g/L, 48.59%, 2.14 g/L/h, and 87.33%, respectively and fermentation time was reduced to 12 h using a biofilm reactor due to high biomass concentration as biofilms. Moreover, the effect of nitrogen sources used in media and non-enriched medium were also investigated. According to results while ethanol production, yield, and maximum production rate were considerably reduced compared to optimized conditions in biofilm reactor, sugar utilization yield was slightly similar. Consequently, whether ethanol production from carob extract in biofilm reactor was victoriously performed, fermentation time was significantly decreased compared to data in the literature.

Original languageEnglish (US)
Title of host publication2016 American Society of Agricultural and Biological Engineers Annual International Meeting, ASABE 2016
PublisherAmerican Society of Agricultural and Biological Engineers
ISBN (Electronic)9781510828759
DOIs
StatePublished - Jan 1 2016
Event2016 ASABE Annual International Meeting - Orlando, United States
Duration: Jul 17 2016Jul 20 2016

Publication series

Name2016 American Society of Agricultural and Biological Engineers Annual International Meeting, ASABE 2016

Other

Other2016 ASABE Annual International Meeting
CountryUnited States
CityOrlando
Period7/17/167/20/16

Fingerprint

carob
Biofilms
ethanol production
Yeast
biofilm
Saccharomyces cerevisiae
Sugars
Ethanol
sugars
extracts
Fermentation
fermentation
Sugar (sucrose)
ethanol
pullulan
beta-mannosidase
batch fermentation
value-added products
agitation
Fructose

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Agronomy and Crop Science

Cite this

Germec, M., Turhan, I., Karhan, M., & Demirci, A. (2016). Ethanol production from carob extract by using Saccharomyces cerevisiae in biofilm reactor. In 2016 American Society of Agricultural and Biological Engineers Annual International Meeting, ASABE 2016 (2016 American Society of Agricultural and Biological Engineers Annual International Meeting, ASABE 2016). American Society of Agricultural and Biological Engineers. https://doi.org/10.13031/aim.20162456236
Germec, Mustafa ; Turhan, Irfan ; Karhan, Mustafa ; Demirci, Ali. / Ethanol production from carob extract by using Saccharomyces cerevisiae in biofilm reactor. 2016 American Society of Agricultural and Biological Engineers Annual International Meeting, ASABE 2016. American Society of Agricultural and Biological Engineers, 2016. (2016 American Society of Agricultural and Biological Engineers Annual International Meeting, ASABE 2016).
@inproceedings{03296fdfe19e401180f916b683795acf,
title = "Ethanol production from carob extract by using Saccharomyces cerevisiae in biofilm reactor",
abstract = "Carob, widely grown in Mediterranean countries, contains several types of sugars such as 34 42{\%} sucrose, 7-10{\%} glucose, and 10-12{\%} fructose. Due to the high sugar concentration, it is used for production of many value added products via fermentation such as ethanol, lactic acid, citric acid, pullulan, and mannanase. In this study, ethanol production conditions from carob extract through repeated-batch fermentation by using Saccharomycess cerevisiae in biofilm reactor were optimized by response surface methodology. Initial sugar concentration (4-10°Bx), agitation speed (100-200 rpm), and pH (5.0-6.0) were used as variable parameters. Results showed that initial sugar concentration had a significant effect on yield and maximum production rate. Optimum conditions were found to be 7.71°Bx, 120 rpm, and 5.18, respectively. After validation experiments ethanol concentration, yield, maximum production rate, and sugar utilization yield were determined as 24.51 g/L, 48.59{\%}, 2.14 g/L/h, and 87.33{\%}, respectively and fermentation time was reduced to 12 h using a biofilm reactor due to high biomass concentration as biofilms. Moreover, the effect of nitrogen sources used in media and non-enriched medium were also investigated. According to results while ethanol production, yield, and maximum production rate were considerably reduced compared to optimized conditions in biofilm reactor, sugar utilization yield was slightly similar. Consequently, whether ethanol production from carob extract in biofilm reactor was victoriously performed, fermentation time was significantly decreased compared to data in the literature.",
author = "Mustafa Germec and Irfan Turhan and Mustafa Karhan and Ali Demirci",
year = "2016",
month = "1",
day = "1",
doi = "10.13031/aim.20162456236",
language = "English (US)",
series = "2016 American Society of Agricultural and Biological Engineers Annual International Meeting, ASABE 2016",
publisher = "American Society of Agricultural and Biological Engineers",
booktitle = "2016 American Society of Agricultural and Biological Engineers Annual International Meeting, ASABE 2016",
address = "United States",

}

Germec, M, Turhan, I, Karhan, M & Demirci, A 2016, Ethanol production from carob extract by using Saccharomyces cerevisiae in biofilm reactor. in 2016 American Society of Agricultural and Biological Engineers Annual International Meeting, ASABE 2016. 2016 American Society of Agricultural and Biological Engineers Annual International Meeting, ASABE 2016, American Society of Agricultural and Biological Engineers, 2016 ASABE Annual International Meeting, Orlando, United States, 7/17/16. https://doi.org/10.13031/aim.20162456236

Ethanol production from carob extract by using Saccharomyces cerevisiae in biofilm reactor. / Germec, Mustafa; Turhan, Irfan; Karhan, Mustafa; Demirci, Ali.

2016 American Society of Agricultural and Biological Engineers Annual International Meeting, ASABE 2016. American Society of Agricultural and Biological Engineers, 2016. (2016 American Society of Agricultural and Biological Engineers Annual International Meeting, ASABE 2016).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Ethanol production from carob extract by using Saccharomyces cerevisiae in biofilm reactor

AU - Germec, Mustafa

AU - Turhan, Irfan

AU - Karhan, Mustafa

AU - Demirci, Ali

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Carob, widely grown in Mediterranean countries, contains several types of sugars such as 34 42% sucrose, 7-10% glucose, and 10-12% fructose. Due to the high sugar concentration, it is used for production of many value added products via fermentation such as ethanol, lactic acid, citric acid, pullulan, and mannanase. In this study, ethanol production conditions from carob extract through repeated-batch fermentation by using Saccharomycess cerevisiae in biofilm reactor were optimized by response surface methodology. Initial sugar concentration (4-10°Bx), agitation speed (100-200 rpm), and pH (5.0-6.0) were used as variable parameters. Results showed that initial sugar concentration had a significant effect on yield and maximum production rate. Optimum conditions were found to be 7.71°Bx, 120 rpm, and 5.18, respectively. After validation experiments ethanol concentration, yield, maximum production rate, and sugar utilization yield were determined as 24.51 g/L, 48.59%, 2.14 g/L/h, and 87.33%, respectively and fermentation time was reduced to 12 h using a biofilm reactor due to high biomass concentration as biofilms. Moreover, the effect of nitrogen sources used in media and non-enriched medium were also investigated. According to results while ethanol production, yield, and maximum production rate were considerably reduced compared to optimized conditions in biofilm reactor, sugar utilization yield was slightly similar. Consequently, whether ethanol production from carob extract in biofilm reactor was victoriously performed, fermentation time was significantly decreased compared to data in the literature.

AB - Carob, widely grown in Mediterranean countries, contains several types of sugars such as 34 42% sucrose, 7-10% glucose, and 10-12% fructose. Due to the high sugar concentration, it is used for production of many value added products via fermentation such as ethanol, lactic acid, citric acid, pullulan, and mannanase. In this study, ethanol production conditions from carob extract through repeated-batch fermentation by using Saccharomycess cerevisiae in biofilm reactor were optimized by response surface methodology. Initial sugar concentration (4-10°Bx), agitation speed (100-200 rpm), and pH (5.0-6.0) were used as variable parameters. Results showed that initial sugar concentration had a significant effect on yield and maximum production rate. Optimum conditions were found to be 7.71°Bx, 120 rpm, and 5.18, respectively. After validation experiments ethanol concentration, yield, maximum production rate, and sugar utilization yield were determined as 24.51 g/L, 48.59%, 2.14 g/L/h, and 87.33%, respectively and fermentation time was reduced to 12 h using a biofilm reactor due to high biomass concentration as biofilms. Moreover, the effect of nitrogen sources used in media and non-enriched medium were also investigated. According to results while ethanol production, yield, and maximum production rate were considerably reduced compared to optimized conditions in biofilm reactor, sugar utilization yield was slightly similar. Consequently, whether ethanol production from carob extract in biofilm reactor was victoriously performed, fermentation time was significantly decreased compared to data in the literature.

UR - http://www.scopus.com/inward/record.url?scp=85009064152&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85009064152&partnerID=8YFLogxK

U2 - 10.13031/aim.20162456236

DO - 10.13031/aim.20162456236

M3 - Conference contribution

AN - SCOPUS:85009064152

T3 - 2016 American Society of Agricultural and Biological Engineers Annual International Meeting, ASABE 2016

BT - 2016 American Society of Agricultural and Biological Engineers Annual International Meeting, ASABE 2016

PB - American Society of Agricultural and Biological Engineers

ER -

Germec M, Turhan I, Karhan M, Demirci A. Ethanol production from carob extract by using Saccharomyces cerevisiae in biofilm reactor. In 2016 American Society of Agricultural and Biological Engineers Annual International Meeting, ASABE 2016. American Society of Agricultural and Biological Engineers. 2016. (2016 American Society of Agricultural and Biological Engineers Annual International Meeting, ASABE 2016). https://doi.org/10.13031/aim.20162456236