Evaluating observer bias and seasonal detection rates in amphibian pathogen eDNA collections by citizen scientists

James Thomas Julian, Gavin W. Glenney, Christopher Rees

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We trained volunteers from conservation organizations to collect environmental DNA (eDNA) from 21 ponds with amphibian communities that had a history of Batrachochytrium dendrobatidis (Bd) and ranavirus (Rv) infections. Volunteers were given sampling kits to filter pond water and preserve eDNA on filter paper, as were the principal investigators (PIs), who made independent collections within 48 h of volunteer collections. Using multi-scale occupancy modeling, we found no evidence to suggest the observer who collected the water sample (volunteer or PI) influenced either the probability of capturing eDNA on a filter or the probability of detecting extracted eDNA in a quantitative PCR (qPCR) reaction. The cumulative detection probability of Bd eDNA at a pond decreased from May through July 2017 because there was a decrease in the probability of detecting eDNA in qPCR reactions. In contrast, cumulative detection probability increased from May to July for Rv due to a higher probability of capturing eDNA on filters later in the year. Our models estimate that both pathogens could be detected with 95% confidence in as few as 5 water samples taken in June or July tested with either 4 or 3 qPCR reactions, respectively. Our eDNA protocols appeared to detect pathogens with 95% confidence using considerably fewer samples than protocols which typically recommend sampling ≥30 individual animals. In addition, eDNA sampling could reduce some biosecurity concerns, jurisdictional and institutional permitting, and stress to biota at ponds.

Original languageEnglish (US)
Pages (from-to)15-24
Number of pages10
JournalDiseases of Aquatic Organisms
Volume134
Issue number1
DOIs
StatePublished - Jan 1 2019

Fingerprint

amphibian
amphibians
pathogen
DNA
pathogens
volunteers
principal investigators
pond
Ranavirus
filter
quantitative polymerase chain reaction
sampling
rate
citizen
detection
water
biosecurity
preserves
biota
animal

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

Cite this

Julian, James Thomas ; Glenney, Gavin W. ; Rees, Christopher. / Evaluating observer bias and seasonal detection rates in amphibian pathogen eDNA collections by citizen scientists. In: Diseases of Aquatic Organisms. 2019 ; Vol. 134, No. 1. pp. 15-24.
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Evaluating observer bias and seasonal detection rates in amphibian pathogen eDNA collections by citizen scientists. / Julian, James Thomas; Glenney, Gavin W.; Rees, Christopher.

In: Diseases of Aquatic Organisms, Vol. 134, No. 1, 01.01.2019, p. 15-24.

Research output: Contribution to journalArticle

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