Evaluation of a chemically inducible promoter for developing a within-plant refuge for resistance management

Sarah L. Bates, Jun Cao, Jian Zhou Zhao, Elizabeth D. Earle, Richard T. Roush, Anthony M. Shelton

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Chemically inducible production of Bacillus thuringiensis (Bt) toxins in transgenic plants may provide considerable benefits in preventing or delaying the evolution of insect resistance to Bt crops by creating within-plant temporal refuges. We examined the effect of inducible cry1Ab expression on survival of different genotypes (RR, RS, and SS) of diamondback moth, Plutella xylostella (L.), in transgenic broccoli, Brassica oleracea L., plants transformed with a PR-1a/cry1Ab expression cassette. Spraying leaves of these plants with the inducer acibenzolar-s-methyl [=benzo (1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester] (ASM) resulted in high levels of Bt toxin, and detached leaves from fully induced plants caused 100% mortality to all instars of P. xylostella SS and RS genotypes. When plants infested with larvae were treated with ASM, only a few larvae that were nearing completion of their development were able to survive the induction process. Signal transduction from ASM-treated leaves to new plant tissue also was evaluated using a larval assay. New foliage that emerged after plants were induced remained toxic to ≥80% of RS larvae up to the fourth new leaf. In whole plant tests, however, induced plants remained protected from larval damage for ≥3 wk. Uninduced PR-1a/cry1Ab plants seemed to produce low levels of Bt that were undetected by an enzyme-linked immunosorbent assay but that resulted in significant fitness costs for susceptible insects. The suitability of PR-1a/cry1Ab broccoli plants for insect resistance management and the requirements of an appropriate inducible promoter are discussed.

Original languageEnglish (US)
Pages (from-to)2188-2194
Number of pages7
JournalJournal of economic entomology
Volume98
Issue number6
DOIs
StatePublished - Dec 2005

Fingerprint

resistance management
refuge
promoter regions
Bacillus thuringiensis
Plutella xylostella
leaves
broccoli
insect
insects
larva
larvae
toxin
toxins
genotype
evaluation
assay
transgenic plant
Brassica oleracea
spraying
plant tissues

All Science Journal Classification (ASJC) codes

  • Ecology
  • Insect Science

Cite this

Bates, Sarah L. ; Cao, Jun ; Zhao, Jian Zhou ; Earle, Elizabeth D. ; Roush, Richard T. ; Shelton, Anthony M. / Evaluation of a chemically inducible promoter for developing a within-plant refuge for resistance management. In: Journal of economic entomology. 2005 ; Vol. 98, No. 6. pp. 2188-2194.
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abstract = "Chemically inducible production of Bacillus thuringiensis (Bt) toxins in transgenic plants may provide considerable benefits in preventing or delaying the evolution of insect resistance to Bt crops by creating within-plant temporal refuges. We examined the effect of inducible cry1Ab expression on survival of different genotypes (RR, RS, and SS) of diamondback moth, Plutella xylostella (L.), in transgenic broccoli, Brassica oleracea L., plants transformed with a PR-1a/cry1Ab expression cassette. Spraying leaves of these plants with the inducer acibenzolar-s-methyl [=benzo (1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester] (ASM) resulted in high levels of Bt toxin, and detached leaves from fully induced plants caused 100{\%} mortality to all instars of P. xylostella SS and RS genotypes. When plants infested with larvae were treated with ASM, only a few larvae that were nearing completion of their development were able to survive the induction process. Signal transduction from ASM-treated leaves to new plant tissue also was evaluated using a larval assay. New foliage that emerged after plants were induced remained toxic to ≥80{\%} of RS larvae up to the fourth new leaf. In whole plant tests, however, induced plants remained protected from larval damage for ≥3 wk. Uninduced PR-1a/cry1Ab plants seemed to produce low levels of Bt that were undetected by an enzyme-linked immunosorbent assay but that resulted in significant fitness costs for susceptible insects. The suitability of PR-1a/cry1Ab broccoli plants for insect resistance management and the requirements of an appropriate inducible promoter are discussed.",
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Evaluation of a chemically inducible promoter for developing a within-plant refuge for resistance management. / Bates, Sarah L.; Cao, Jun; Zhao, Jian Zhou; Earle, Elizabeth D.; Roush, Richard T.; Shelton, Anthony M.

In: Journal of economic entomology, Vol. 98, No. 6, 12.2005, p. 2188-2194.

Research output: Contribution to journalArticle

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