Evaluation of a commercial enzyme-linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl

Camille Lebarbenchon, Mary Pantin-Jackwood, Whitney M. Kistler, M. Page Luttrell, Erica Spackman, David E. Stallknecht, Justin David Brown

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The ID Screen Influenza H5 Antibody Competition enzyme-linked immunosorbent assay was tested for the detection of antibodies to the H5 subtype of influenza A (IA) virus in waterfowl. Assays were conducted with sera obtained from Mallards (Anas platyrhynchos) and Pekin Ducks (Anas platyrhynchos domestica), experimentally infected with eight low pathogenic (LP) and nine highly pathogenic (HP) H5N1 IA viral strains. Three incubation periods (1, 4 and 18 hours) and two dilutions (1:2 and 1:5) were tested. All serum samples from LP H5-infected birds tested positive; however, improved detection rates were observed for viruses belonging to the HP H5N1 clade 2.2.1 as compared with those belonging to clade 2.1.3.

Original languageEnglish (US)
Pages (from-to)1237-1240
Number of pages4
JournalInfluenza and other Respiratory Viruses
Volume7
Issue number6
DOIs
StatePublished - Nov 1 2013

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Influenza A virus
Human Influenza
Enzyme-Linked Immunosorbent Assay
Ducks
Antibodies
Serum
Birds
Viruses

All Science Journal Classification (ASJC) codes

  • Epidemiology
  • Pulmonary and Respiratory Medicine
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Lebarbenchon, C., Pantin-Jackwood, M., Kistler, W. M., Page Luttrell, M., Spackman, E., Stallknecht, D. E., & Brown, J. D. (2013). Evaluation of a commercial enzyme-linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl. Influenza and other Respiratory Viruses, 7(6), 1237-1240. https://doi.org/10.1111/irv.12070
Lebarbenchon, Camille ; Pantin-Jackwood, Mary ; Kistler, Whitney M. ; Page Luttrell, M. ; Spackman, Erica ; Stallknecht, David E. ; Brown, Justin David. / Evaluation of a commercial enzyme-linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl. In: Influenza and other Respiratory Viruses. 2013 ; Vol. 7, No. 6. pp. 1237-1240.
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Lebarbenchon, C, Pantin-Jackwood, M, Kistler, WM, Page Luttrell, M, Spackman, E, Stallknecht, DE & Brown, JD 2013, 'Evaluation of a commercial enzyme-linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl', Influenza and other Respiratory Viruses, vol. 7, no. 6, pp. 1237-1240. https://doi.org/10.1111/irv.12070

Evaluation of a commercial enzyme-linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl. / Lebarbenchon, Camille; Pantin-Jackwood, Mary; Kistler, Whitney M.; Page Luttrell, M.; Spackman, Erica; Stallknecht, David E.; Brown, Justin David.

In: Influenza and other Respiratory Viruses, Vol. 7, No. 6, 01.11.2013, p. 1237-1240.

Research output: Contribution to journalArticle

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