Evaluation of a commercial enzyme-linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl

Camille Lebarbenchon, Mary Pantin-Jackwood, Whitney M. Kistler, M. Page Luttrell, Erica Spackman, David E. Stallknecht, Justin D. Brown

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The ID Screen Influenza H5 Antibody Competition enzyme-linked immunosorbent assay was tested for the detection of antibodies to the H5 subtype of influenza A (IA) virus in waterfowl. Assays were conducted with sera obtained from Mallards (Anas platyrhynchos) and Pekin Ducks (Anas platyrhynchos domestica), experimentally infected with eight low pathogenic (LP) and nine highly pathogenic (HP) H5N1 IA viral strains. Three incubation periods (1, 4 and 18 hours) and two dilutions (1:2 and 1:5) were tested. All serum samples from LP H5-infected birds tested positive; however, improved detection rates were observed for viruses belonging to the HP H5N1 clade 2.2.1 as compared with those belonging to clade 2.1.3.

Original languageEnglish (US)
Pages (from-to)1237-1240
Number of pages4
JournalInfluenza and other Respiratory Viruses
Volume7
Issue number6
DOIs
StatePublished - Nov 2013

All Science Journal Classification (ASJC) codes

  • Epidemiology
  • Pulmonary and Respiratory Medicine
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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