Evaluation of benzo[c]chrysene dihydrodiols in the morphological cell transformation of mouse embryo fibroblast C3H10T1/2CL8 cells

S. Nesnow, C. Davis, Dhimant Desai, Shantu Amin

Research output: Contribution to journalReview article

1 Citation (Scopus)

Abstract

The morphological cell transforming activities of three dihydrodiols of benzo[c]chrysene (B[c]C), trans-B[c]C-7,8-diol, trans-B[c]C-9,10-diol, and trans-B[c]C-1,2-diol were compared to those of B[c]C in order to study the possible routes of metabolic activation in transformable C3H10T1/2 mouse embryo fibroblasts. B[c]C-treated C3H10T1/2 cells exhibited a concentration-related increase in morphologically transformed foci over a concentration range of 0-3 μg/ml. At 3 μg/ml, B[c]C induced 1.23 Type II&III foci/dish, with 73% of the dishes exhibiting Type II or Type III foci, and a survival of 87%. trans-B[c]C-7,8-diol produced concentration-related responses over a range of 0-5 μg/ml. At 3 μg/ml, trans-B[c]C-7,8-diol produced 1.13 Type II&III foci/dish with 72% of the dishes exhibiting foci, and a survival of 76%. trans-B[c]C-9,10-diol was inactive as a morphological cell transforming agent over a concentration range of 0-3 μg/ml. trans-B[c]C-1,2-diol was also inactive as a morphological cell transforming agent over a concentration range of 0-3 μg/ml. These results suggest that a K-region dihydrodiol of B[c]C, trans-B[c]C-7,8-diol, may play a role in the ability of B[c]C to morphologically transform C3H10T1/2 cells.

Original languageEnglish (US)
Pages (from-to)203-213
Number of pages11
JournalPolycyclic Aromatic Compounds
Volume21
Issue number1-4
DOIs
StatePublished - Jan 1 2000

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Fibroblasts
Chemical activation
benzo(c)chrysene
trans-1,2-dihydro-1,2-naphthalenediol

All Science Journal Classification (ASJC) codes

  • Polymers and Plastics
  • Organic Chemistry
  • Materials Chemistry

Cite this

@article{8a8b3e3e9e784923b6741c1083ba83bc,
title = "Evaluation of benzo[c]chrysene dihydrodiols in the morphological cell transformation of mouse embryo fibroblast C3H10T1/2CL8 cells",
abstract = "The morphological cell transforming activities of three dihydrodiols of benzo[c]chrysene (B[c]C), trans-B[c]C-7,8-diol, trans-B[c]C-9,10-diol, and trans-B[c]C-1,2-diol were compared to those of B[c]C in order to study the possible routes of metabolic activation in transformable C3H10T1/2 mouse embryo fibroblasts. B[c]C-treated C3H10T1/2 cells exhibited a concentration-related increase in morphologically transformed foci over a concentration range of 0-3 μg/ml. At 3 μg/ml, B[c]C induced 1.23 Type II&III foci/dish, with 73{\%} of the dishes exhibiting Type II or Type III foci, and a survival of 87{\%}. trans-B[c]C-7,8-diol produced concentration-related responses over a range of 0-5 μg/ml. At 3 μg/ml, trans-B[c]C-7,8-diol produced 1.13 Type II&III foci/dish with 72{\%} of the dishes exhibiting foci, and a survival of 76{\%}. trans-B[c]C-9,10-diol was inactive as a morphological cell transforming agent over a concentration range of 0-3 μg/ml. trans-B[c]C-1,2-diol was also inactive as a morphological cell transforming agent over a concentration range of 0-3 μg/ml. These results suggest that a K-region dihydrodiol of B[c]C, trans-B[c]C-7,8-diol, may play a role in the ability of B[c]C to morphologically transform C3H10T1/2 cells.",
author = "S. Nesnow and C. Davis and Dhimant Desai and Shantu Amin",
year = "2000",
month = "1",
day = "1",
doi = "10.1080/10406630008028534",
language = "English (US)",
volume = "21",
pages = "203--213",
journal = "Polycyclic Aromatic Compounds",
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}

Evaluation of benzo[c]chrysene dihydrodiols in the morphological cell transformation of mouse embryo fibroblast C3H10T1/2CL8 cells. / Nesnow, S.; Davis, C.; Desai, Dhimant; Amin, Shantu.

In: Polycyclic Aromatic Compounds, Vol. 21, No. 1-4, 01.01.2000, p. 203-213.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Evaluation of benzo[c]chrysene dihydrodiols in the morphological cell transformation of mouse embryo fibroblast C3H10T1/2CL8 cells

AU - Nesnow, S.

AU - Davis, C.

AU - Desai, Dhimant

AU - Amin, Shantu

PY - 2000/1/1

Y1 - 2000/1/1

N2 - The morphological cell transforming activities of three dihydrodiols of benzo[c]chrysene (B[c]C), trans-B[c]C-7,8-diol, trans-B[c]C-9,10-diol, and trans-B[c]C-1,2-diol were compared to those of B[c]C in order to study the possible routes of metabolic activation in transformable C3H10T1/2 mouse embryo fibroblasts. B[c]C-treated C3H10T1/2 cells exhibited a concentration-related increase in morphologically transformed foci over a concentration range of 0-3 μg/ml. At 3 μg/ml, B[c]C induced 1.23 Type II&III foci/dish, with 73% of the dishes exhibiting Type II or Type III foci, and a survival of 87%. trans-B[c]C-7,8-diol produced concentration-related responses over a range of 0-5 μg/ml. At 3 μg/ml, trans-B[c]C-7,8-diol produced 1.13 Type II&III foci/dish with 72% of the dishes exhibiting foci, and a survival of 76%. trans-B[c]C-9,10-diol was inactive as a morphological cell transforming agent over a concentration range of 0-3 μg/ml. trans-B[c]C-1,2-diol was also inactive as a morphological cell transforming agent over a concentration range of 0-3 μg/ml. These results suggest that a K-region dihydrodiol of B[c]C, trans-B[c]C-7,8-diol, may play a role in the ability of B[c]C to morphologically transform C3H10T1/2 cells.

AB - The morphological cell transforming activities of three dihydrodiols of benzo[c]chrysene (B[c]C), trans-B[c]C-7,8-diol, trans-B[c]C-9,10-diol, and trans-B[c]C-1,2-diol were compared to those of B[c]C in order to study the possible routes of metabolic activation in transformable C3H10T1/2 mouse embryo fibroblasts. B[c]C-treated C3H10T1/2 cells exhibited a concentration-related increase in morphologically transformed foci over a concentration range of 0-3 μg/ml. At 3 μg/ml, B[c]C induced 1.23 Type II&III foci/dish, with 73% of the dishes exhibiting Type II or Type III foci, and a survival of 87%. trans-B[c]C-7,8-diol produced concentration-related responses over a range of 0-5 μg/ml. At 3 μg/ml, trans-B[c]C-7,8-diol produced 1.13 Type II&III foci/dish with 72% of the dishes exhibiting foci, and a survival of 76%. trans-B[c]C-9,10-diol was inactive as a morphological cell transforming agent over a concentration range of 0-3 μg/ml. trans-B[c]C-1,2-diol was also inactive as a morphological cell transforming agent over a concentration range of 0-3 μg/ml. These results suggest that a K-region dihydrodiol of B[c]C, trans-B[c]C-7,8-diol, may play a role in the ability of B[c]C to morphologically transform C3H10T1/2 cells.

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DO - 10.1080/10406630008028534

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