Evaluation of Direct PCR Amplification Using Various Swabs and Washing Reagents

Hallie Altshuler, Reena Roy

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

DNA profiles were generated via direct amplification from blood and saliva samples deposited on various types of swab substrates. Each of the six non-FTA substrates used in this research was punched with a Harris 1.2 mm puncher. After 0.1 μL of blood or 0.5 μL saliva, samples were deposited on each of these punches, samples were pretreated with one of four buffers and washing reagents. Amplification was performed using direct and nondirect autosomal and Y-STR kits. Autosomal and Y-STR profiles were successfully generated from most of these substrates when pretreated with buffer or washing reagents. Concordant profiles were obtained within and between the six substrates, the six amplification kits, and all four reagents. The direct amplification of substrates which do not contain lysing agent would be beneficial to the forensic community as the procedure can be used on evidence samples commonly found at crime scenes.

Original languageEnglish (US)
Pages (from-to)1542-1552
Number of pages11
JournalJournal of Forensic Sciences
Volume60
Issue number6
DOIs
StatePublished - Nov 1 2015

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Saliva
Polymerase Chain Reaction
Buffers
Crime
DNA
Research

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Genetics

Cite this

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Evaluation of Direct PCR Amplification Using Various Swabs and Washing Reagents. / Altshuler, Hallie; Roy, Reena.

In: Journal of Forensic Sciences, Vol. 60, No. 6, 01.11.2015, p. 1542-1552.

Research output: Contribution to journalArticle

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