The cleanliness and sanitation of the on-farm milking system directly affects raw milk quality. A four-step procedure is generally accepted for cleaning-in-place (CIP) of milking systems, which involves water rinse, hot alkaline wash, warm acid wash and sanitizing wash. The electrolyzed oxidizing (EO) water is a technology in which acidic EO water and alkaline EO water solutions are generated separately by electrolyzing weak sodium chloride solution. As these two solutions match the basic requirements for the CIP of milking systems, it was proposed that the EO water can be used as a cleaning and sanitizing agent for CIP of milking systems. Previous studies demonstrated that the utilization of the EO water CIP provided equal or better results than the conventional CIP in a pilot-scale milking system. This research was undertaken to evaluate, assess, and validate the EO water technology on a commercial dairy farm compared with the conventional CIP. Results from the Adenosine Tri-Phosphate (ATP) bioluminescence method showed that the EO water CIP provided equal to or better results compared with conventional CIP for most of the sampling locations and other system components. Moreover, results for bacterial presence corresponded with the ATP bioluminescence results, indicating the capability of using EO water for the cleaning and sanitizing of on-farm milking systems. For one complete CIP operational process, it was estimated that the cost of using the EO water CIP is lower than using the conventional CIP by about 25%. Based on the technical performance and economic analyses, this study showed that the EO water has the potential to be adapted as an alternative CIP of on-farm milking systems.