Evaluation of immunocontraceptive adjuvants, titers, and fecal pregnancy indicators in free-ranging white-tailed deer

W. David Walter, Peter J. Pekins, Allen T. Rutberg, Howard J. Kilpatrick

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The future of the porcine zona pellucida (PZP) immunocontraceptive in white-tailed deer (Odocoileus virginianus) is dependent upon finding an adjuvant that is highly immunogenic, resulting in reproductive inhibition. Antibody measurement in plasma, fawn-doe observations, and fecal pregnancy indicators have been used to assess PZP effectiveness and reproduction in ungulates. During 1997-1998, potentially reproductive (>1 year) female white-tailed deer in a suburban Connecticut community were immobilized and received 1 of 3 treatments: 1) synthetic trehalose dicorynmycolate (S-TDCM) plus PZP, 2) Freund's Complete and Incomplete Adjuvants (FCA) plus PZP, and 3) FCA plus sterile water (control). Fawn-doe observations indicated that 5 of 9 S-TDCM-treated and 1 of 9 FCA-treated deer reproduced and reproduction was similar (P=0.13); 78% reproduction by control deer was higher than FCA deer (P<0.001), but similar to S-TDCM deer (P=0.20). Mean percent titers in blood collected 4-5 months post-treatment were higher in FCA deer (100±7) than those of S-TDCM (32±6) and control (18±2) deer (P<0.001). The S-TDCM adjuvant appeared less effective than the FCA adjuvant at preventing conception and maintaining titer levels with PZP. In spring, fecal progesterone metabolite concentrations (PdG) ranged from (0.16-17.6 μg/g dmf) and were similar for pregnant and nonpregnant deer in 1998 (P=0.13) and 1999 (P=0.84). Failure to identify pregnancy status using PdG may have been influenced by the variability in progesterone production by continually cycling and pregnant deer.

Original languageEnglish (US)
Pages (from-to)908-914
Number of pages7
JournalWildlife Society Bulletin
Volume30
Issue number3
StatePublished - Sep 2002

Fingerprint

Odocoileus virginianus
pregnancy
deer
adjuvants
zona pellucida
trehalose
does (females)
swine
fawns
progesterone
evaluation
indicator
ungulates
ungulate
antibody
metabolite
metabolites
blood
antibodies
plasma

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • Ecology

Cite this

Walter, W. David ; Pekins, Peter J. ; Rutberg, Allen T. ; Kilpatrick, Howard J. / Evaluation of immunocontraceptive adjuvants, titers, and fecal pregnancy indicators in free-ranging white-tailed deer. In: Wildlife Society Bulletin. 2002 ; Vol. 30, No. 3. pp. 908-914.
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abstract = "The future of the porcine zona pellucida (PZP) immunocontraceptive in white-tailed deer (Odocoileus virginianus) is dependent upon finding an adjuvant that is highly immunogenic, resulting in reproductive inhibition. Antibody measurement in plasma, fawn-doe observations, and fecal pregnancy indicators have been used to assess PZP effectiveness and reproduction in ungulates. During 1997-1998, potentially reproductive (>1 year) female white-tailed deer in a suburban Connecticut community were immobilized and received 1 of 3 treatments: 1) synthetic trehalose dicorynmycolate (S-TDCM) plus PZP, 2) Freund's Complete and Incomplete Adjuvants (FCA) plus PZP, and 3) FCA plus sterile water (control). Fawn-doe observations indicated that 5 of 9 S-TDCM-treated and 1 of 9 FCA-treated deer reproduced and reproduction was similar (P=0.13); 78{\%} reproduction by control deer was higher than FCA deer (P<0.001), but similar to S-TDCM deer (P=0.20). Mean percent titers in blood collected 4-5 months post-treatment were higher in FCA deer (100±7) than those of S-TDCM (32±6) and control (18±2) deer (P<0.001). The S-TDCM adjuvant appeared less effective than the FCA adjuvant at preventing conception and maintaining titer levels with PZP. In spring, fecal progesterone metabolite concentrations (PdG) ranged from (0.16-17.6 μg/g dmf) and were similar for pregnant and nonpregnant deer in 1998 (P=0.13) and 1999 (P=0.84). Failure to identify pregnancy status using PdG may have been influenced by the variability in progesterone production by continually cycling and pregnant deer.",
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Evaluation of immunocontraceptive adjuvants, titers, and fecal pregnancy indicators in free-ranging white-tailed deer. / Walter, W. David; Pekins, Peter J.; Rutberg, Allen T.; Kilpatrick, Howard J.

In: Wildlife Society Bulletin, Vol. 30, No. 3, 09.2002, p. 908-914.

Research output: Contribution to journalArticle

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