Evaluation of liquid chromatography-atmospheric pressure chemical ionisation-mass spectrometry for the identification and quantification of desulphoglucosinolates

D. Wynne Griffiths, Henry Bain, Nigel Deighton, Nigel P. Botting, Avril A.B. Robertson

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The use of liquid chromatography-atmospheric pressure chemical ionisation-mass spectrometry has been investigated as a potential method for both the identification and quantification of desulphoglucosinolates. The results indicate that this methodology is particularly useful as a rapid technique for the confirmation of the identity of desulphoglucosinolates and has, in full scan mode, limits of detection at least an order of magnitude lower than is normally achieved by conventional high pressure liquid chromatography with UV detection. It has been demonstrated that, using a per-deuterated desulphoglucosinolate as an internal standard, quantification could be achieved, but for accurate analysis at low concentrations it would be necessary to produce independent calibration curves for each desulphoglucosinolate present in the sample. Copyright (C) 2000 John Wiley and Sons, Ltd.

Original languageEnglish (US)
Pages (from-to)216-225
Number of pages10
JournalPhytochemical Analysis
Volume11
Issue number4
DOIs
StatePublished - Jul 1 2000

Fingerprint

High pressure liquid chromatography
Atmospheric Pressure
Liquid chromatography
atmospheric pressure
Liquid Chromatography
ionization
liquid chromatography
Calibration
Atmospheric pressure
Ionization
Mass spectrometry
Limit of Detection
Mass Spectrometry
High Pressure Liquid Chromatography
mass spectrometry
detection limit
calibration
high performance liquid chromatography
methodology
sampling

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Food Science
  • Biochemistry
  • Molecular Medicine
  • Plant Science
  • Drug Discovery
  • Complementary and alternative medicine

Cite this

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Evaluation of liquid chromatography-atmospheric pressure chemical ionisation-mass spectrometry for the identification and quantification of desulphoglucosinolates. / Griffiths, D. Wynne; Bain, Henry; Deighton, Nigel; Botting, Nigel P.; Robertson, Avril A.B.

In: Phytochemical Analysis, Vol. 11, No. 4, 01.07.2000, p. 216-225.

Research output: Contribution to journalArticle

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T1 - Evaluation of liquid chromatography-atmospheric pressure chemical ionisation-mass spectrometry for the identification and quantification of desulphoglucosinolates

AU - Griffiths, D. Wynne

AU - Bain, Henry

AU - Deighton, Nigel

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AU - Robertson, Avril A.B.

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AB - The use of liquid chromatography-atmospheric pressure chemical ionisation-mass spectrometry has been investigated as a potential method for both the identification and quantification of desulphoglucosinolates. The results indicate that this methodology is particularly useful as a rapid technique for the confirmation of the identity of desulphoglucosinolates and has, in full scan mode, limits of detection at least an order of magnitude lower than is normally achieved by conventional high pressure liquid chromatography with UV detection. It has been demonstrated that, using a per-deuterated desulphoglucosinolate as an internal standard, quantification could be achieved, but for accurate analysis at low concentrations it would be necessary to produce independent calibration curves for each desulphoglucosinolate present in the sample. Copyright (C) 2000 John Wiley and Sons, Ltd.

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