Evaluation of the biological properties and cross-reactive antibody response to h10 influenza viruses in ferrets

Troy C. Sutton, Elaine W. Lamirande, Rita Czako, Kanta Subbarao

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The recent outbreak of avian origin H10N7 influenza among seals in northern Europe and two fatal human infections with an avian H10N8 virus in China have demonstrated that H10 viruses can spread between mammals and cause severe disease in humans. To gain insight into the potential for H10 viruses to cross the species barrier and to identify a candidate vaccine strain, we evaluated the in vitro and in vivo properties and antibody response in ferrets to 20 diverse H10 viruses. H10 virus infection of ferrets caused variable weight loss, and all 20 viruses replicated throughout the respiratory tract; however, replication in the lungs was highly variable. In glycan-binding assays, the H10 viruses preferentially bound "avianlike" α2,3-linked sialic acids. Importantly, several isolates also displayed strong binding to long-chain "human-like" α2,6-linked sialic acids and exhibited comparable or elevated neuraminidase activity relative to human H1N1, H2N2, and H3N2 viruses. In hemagglutination inhibition assays, 12 antisera cross-reacted with ≥14 of 20 H10 viruses, and 7 viruses induced neutralizing activity against ≥15 of the 20 viruses. By combining data on weight loss, viral replication, and the cross-reactive antibody response, we identified A/mallard/Portugal/79906/2009 (H10N7) as a suitable virus for vaccine development. Collectively, our findings suggest that H10 viruses may continue to sporadically infect humans and other mammals, underscoring the importance of developing an H10 vaccine for pandemic preparedness.

Original languageEnglish (US)
Article numbere00895-17
JournalJournal of virology
Volume91
Issue number19
DOIs
StatePublished - Oct 1 2017

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Ferrets
ferrets
Orthomyxoviridae
Antibody Formation
Viruses
viruses
antibodies
Sialic Acids
Influenza A virus
Vaccines
sialic acids
H2N2 Subtype Influenza A Virus
Weight Loss
Mammals
H3N2 Subtype Influenza A Virus
weight loss
H1N1 Subtype Influenza A Virus
Portugal
mammals
vaccines

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

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abstract = "The recent outbreak of avian origin H10N7 influenza among seals in northern Europe and two fatal human infections with an avian H10N8 virus in China have demonstrated that H10 viruses can spread between mammals and cause severe disease in humans. To gain insight into the potential for H10 viruses to cross the species barrier and to identify a candidate vaccine strain, we evaluated the in vitro and in vivo properties and antibody response in ferrets to 20 diverse H10 viruses. H10 virus infection of ferrets caused variable weight loss, and all 20 viruses replicated throughout the respiratory tract; however, replication in the lungs was highly variable. In glycan-binding assays, the H10 viruses preferentially bound {"}avianlike{"} α2,3-linked sialic acids. Importantly, several isolates also displayed strong binding to long-chain {"}human-like{"} α2,6-linked sialic acids and exhibited comparable or elevated neuraminidase activity relative to human H1N1, H2N2, and H3N2 viruses. In hemagglutination inhibition assays, 12 antisera cross-reacted with ≥14 of 20 H10 viruses, and 7 viruses induced neutralizing activity against ≥15 of the 20 viruses. By combining data on weight loss, viral replication, and the cross-reactive antibody response, we identified A/mallard/Portugal/79906/2009 (H10N7) as a suitable virus for vaccine development. Collectively, our findings suggest that H10 viruses may continue to sporadically infect humans and other mammals, underscoring the importance of developing an H10 vaccine for pandemic preparedness.",
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Evaluation of the biological properties and cross-reactive antibody response to h10 influenza viruses in ferrets. / Sutton, Troy C.; Lamirande, Elaine W.; Czako, Rita; Subbarao, Kanta.

In: Journal of virology, Vol. 91, No. 19, e00895-17, 01.10.2017.

Research output: Contribution to journalArticle

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