Evaluation of the experimental parameters which control electron detachment dissociation, and their effect on the fragmentation efficiency of glycosaminoglycan carbohydrates

Franklin E. Leach, Jeremy J. Wolff, Tatiana N. Laremore, Robert J. Linhardt, I. Jonathan Amster

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The efficiency of conversion of precursor ions to observable products for electron detachment dissociation (EDD) was measured as a function of the key experimental parameters to determine their optimal values for the Fourier transform mass spectrometry analysis of anionic glycosaminoglycan carbohydrates. These parameters include electron current, electron energy, dispenser cathode heater current, electron beam duration, charge state of the precursor ion, oligomer length, and precursor ion number accumulated in an external radio frequency multipole trap. Precursor conversion is most efficient at an electron current of 15 μA, and decreases at higher and lower values. The conversion of precursor to product ions increases in efficiency as the electron pulse duration is increased. Together, these data suggest that a radially repulsive electric field is produced between the electron beam and negative ions during EDD which causes the reaction cross-section to decrease at higher values of electron current (>15 μA). Elevating the heater current of the dispenser cathode increases the electron flux, but also causes ion activation, presumably by blackbody infrared irradiation. An electronic circuit is described that allows the electron current produced by the dispenser cathode to be measured during an EDD or electron capture dissociation (ECD) experiment.

Original languageEnglish (US)
Pages (from-to)110-115
Number of pages6
JournalInternational Journal of Mass Spectrometry
Volume276
Issue number2-3
DOIs
StatePublished - Oct 1 2008

Fingerprint

carbohydrates
Carbohydrates
Glycosaminoglycans
detachment
fragmentation
dissociation
Electrons
evaluation
dispensers
electrons
Dispensers
Ions
ions
cathodes
heaters
Cathodes
electron beams
Electron beams
electron flux
causes

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Condensed Matter Physics
  • Spectroscopy
  • Physical and Theoretical Chemistry

Cite this

@article{07a66b2b6cad4a23b47d0fa543654c25,
title = "Evaluation of the experimental parameters which control electron detachment dissociation, and their effect on the fragmentation efficiency of glycosaminoglycan carbohydrates",
abstract = "The efficiency of conversion of precursor ions to observable products for electron detachment dissociation (EDD) was measured as a function of the key experimental parameters to determine their optimal values for the Fourier transform mass spectrometry analysis of anionic glycosaminoglycan carbohydrates. These parameters include electron current, electron energy, dispenser cathode heater current, electron beam duration, charge state of the precursor ion, oligomer length, and precursor ion number accumulated in an external radio frequency multipole trap. Precursor conversion is most efficient at an electron current of 15 μA, and decreases at higher and lower values. The conversion of precursor to product ions increases in efficiency as the electron pulse duration is increased. Together, these data suggest that a radially repulsive electric field is produced between the electron beam and negative ions during EDD which causes the reaction cross-section to decrease at higher values of electron current (>15 μA). Elevating the heater current of the dispenser cathode increases the electron flux, but also causes ion activation, presumably by blackbody infrared irradiation. An electronic circuit is described that allows the electron current produced by the dispenser cathode to be measured during an EDD or electron capture dissociation (ECD) experiment.",
author = "Leach, {Franklin E.} and Wolff, {Jeremy J.} and Laremore, {Tatiana N.} and Linhardt, {Robert J.} and Amster, {I. Jonathan}",
year = "2008",
month = "10",
day = "1",
doi = "10.1016/j.ijms.2008.05.017",
language = "English (US)",
volume = "276",
pages = "110--115",
journal = "International Journal of Mass Spectrometry",
issn = "1387-3806",
publisher = "Elsevier",
number = "2-3",

}

Evaluation of the experimental parameters which control electron detachment dissociation, and their effect on the fragmentation efficiency of glycosaminoglycan carbohydrates. / Leach, Franklin E.; Wolff, Jeremy J.; Laremore, Tatiana N.; Linhardt, Robert J.; Amster, I. Jonathan.

In: International Journal of Mass Spectrometry, Vol. 276, No. 2-3, 01.10.2008, p. 110-115.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Evaluation of the experimental parameters which control electron detachment dissociation, and their effect on the fragmentation efficiency of glycosaminoglycan carbohydrates

AU - Leach, Franklin E.

AU - Wolff, Jeremy J.

AU - Laremore, Tatiana N.

AU - Linhardt, Robert J.

AU - Amster, I. Jonathan

PY - 2008/10/1

Y1 - 2008/10/1

N2 - The efficiency of conversion of precursor ions to observable products for electron detachment dissociation (EDD) was measured as a function of the key experimental parameters to determine their optimal values for the Fourier transform mass spectrometry analysis of anionic glycosaminoglycan carbohydrates. These parameters include electron current, electron energy, dispenser cathode heater current, electron beam duration, charge state of the precursor ion, oligomer length, and precursor ion number accumulated in an external radio frequency multipole trap. Precursor conversion is most efficient at an electron current of 15 μA, and decreases at higher and lower values. The conversion of precursor to product ions increases in efficiency as the electron pulse duration is increased. Together, these data suggest that a radially repulsive electric field is produced between the electron beam and negative ions during EDD which causes the reaction cross-section to decrease at higher values of electron current (>15 μA). Elevating the heater current of the dispenser cathode increases the electron flux, but also causes ion activation, presumably by blackbody infrared irradiation. An electronic circuit is described that allows the electron current produced by the dispenser cathode to be measured during an EDD or electron capture dissociation (ECD) experiment.

AB - The efficiency of conversion of precursor ions to observable products for electron detachment dissociation (EDD) was measured as a function of the key experimental parameters to determine their optimal values for the Fourier transform mass spectrometry analysis of anionic glycosaminoglycan carbohydrates. These parameters include electron current, electron energy, dispenser cathode heater current, electron beam duration, charge state of the precursor ion, oligomer length, and precursor ion number accumulated in an external radio frequency multipole trap. Precursor conversion is most efficient at an electron current of 15 μA, and decreases at higher and lower values. The conversion of precursor to product ions increases in efficiency as the electron pulse duration is increased. Together, these data suggest that a radially repulsive electric field is produced between the electron beam and negative ions during EDD which causes the reaction cross-section to decrease at higher values of electron current (>15 μA). Elevating the heater current of the dispenser cathode increases the electron flux, but also causes ion activation, presumably by blackbody infrared irradiation. An electronic circuit is described that allows the electron current produced by the dispenser cathode to be measured during an EDD or electron capture dissociation (ECD) experiment.

UR - http://www.scopus.com/inward/record.url?scp=50849102623&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=50849102623&partnerID=8YFLogxK

U2 - 10.1016/j.ijms.2008.05.017

DO - 10.1016/j.ijms.2008.05.017

M3 - Article

AN - SCOPUS:50849102623

VL - 276

SP - 110

EP - 115

JO - International Journal of Mass Spectrometry

JF - International Journal of Mass Spectrometry

SN - 1387-3806

IS - 2-3

ER -