Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam [version 1; referees: 2 approved]

VIZIONS consortium

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Abstract

Background: Acute respiratory infections (ARI) are among the leading causes of hospitalization in children ≤5 years old. Rapid diagnostics of viral pathogens is essential to avoid unnecessary antibiotic treatment, thereby slowing down antibiotic-resistance. We evaluated the diagnostic performance of the Luminex xTAG Respiratory Viral Panel FAST v2 against viral specific PCR as reference assays for ARI in Vietnam. Methods: Four hundred and forty two nose and throat swabs were collected in viral transport medium, and were tested with Luminex xTAG Respiratory Viral Panel FAST v2. Multiplex RT-PCR and single RT-PCR were used as references. Results: Overall, viral pathogens were detected in a total count of 270/294 (91.8%, 95% CI 88.1-94.7) by the Luminex among reference assays, whilst 112/6336 (1.8%, 95% CI, 1.4-2.1) of pathogens were detected by the Luminex, but not by reference assays. Frequency of pathogens detected by Luminex and reference assays was 379 and 292, respectively. The diagnostic yield was 66.7% (295/442, 95%CI 62.1-71.1%) for the Luminex assay and 54.1% (239/442, 95% CI, 49.3-58.8%) for reference assays. The Luminex kit had higher yields for all viruses except influenza B virus, respiratory syncytial virus, and human bocavirus. High agreements between both methods [mean (range): 0.91 (0.83-1.00)] were found for 10/15 viral agents. Conclusions: The Luminex assay is a high throughput multiplex platform for rapid detection of common viral pathogens causing ARI. Although the current high cost may prevent Luminex assays from being widely used, especially in limited resource settings where ARI are felt most, its introduction in clinical diagnostics may help reduce unnecessary use of antibiotic prescription.

Original languageEnglish (US)
Article number80
JournalWellcome Open Research
Volume2
DOIs
StatePublished - Jan 1 2017

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Vietnam
Pathogens
Pharynx
Nose
Respiratory Tract Infections
Assays
Human bocavirus
Viruses
Anti-Bacterial Agents
Influenza B virus
Polymerase Chain Reaction
Respiratory Syncytial Viruses
Multiplex Polymerase Chain Reaction
Microbial Drug Resistance
Prescriptions
Hospitalization
Costs and Cost Analysis
Throughput
Therapeutics

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

@article{4e1331d096dd4af49efa7b8e09874a2d,
title = "Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam [version 1; referees: 2 approved]",
abstract = "Background: Acute respiratory infections (ARI) are among the leading causes of hospitalization in children ≤5 years old. Rapid diagnostics of viral pathogens is essential to avoid unnecessary antibiotic treatment, thereby slowing down antibiotic-resistance. We evaluated the diagnostic performance of the Luminex xTAG Respiratory Viral Panel FAST v2 against viral specific PCR as reference assays for ARI in Vietnam. Methods: Four hundred and forty two nose and throat swabs were collected in viral transport medium, and were tested with Luminex xTAG Respiratory Viral Panel FAST v2. Multiplex RT-PCR and single RT-PCR were used as references. Results: Overall, viral pathogens were detected in a total count of 270/294 (91.8{\%}, 95{\%} CI 88.1-94.7) by the Luminex among reference assays, whilst 112/6336 (1.8{\%}, 95{\%} CI, 1.4-2.1) of pathogens were detected by the Luminex, but not by reference assays. Frequency of pathogens detected by Luminex and reference assays was 379 and 292, respectively. The diagnostic yield was 66.7{\%} (295/442, 95{\%}CI 62.1-71.1{\%}) for the Luminex assay and 54.1{\%} (239/442, 95{\%} CI, 49.3-58.8{\%}) for reference assays. The Luminex kit had higher yields for all viruses except influenza B virus, respiratory syncytial virus, and human bocavirus. High agreements between both methods [mean (range): 0.91 (0.83-1.00)] were found for 10/15 viral agents. Conclusions: The Luminex assay is a high throughput multiplex platform for rapid detection of common viral pathogens causing ARI. Although the current high cost may prevent Luminex assays from being widely used, especially in limited resource settings where ARI are felt most, its introduction in clinical diagnostics may help reduce unnecessary use of antibiotic prescription.",
author = "{VIZIONS consortium} and {Thi Ty Hang}, Vu and {Thi Han Ny}, Nguyen and {My Phuc}, Tran and {Thi Thanh Tam}, Pham and {Thao Huong}, Dang and {Dang Trung Nghia}, Ho and {Tran Anh Vu}, Nguyen and {Thi Hong Phuong}, Pham and {Van Xang}, Nguyen and Nguyen Dong and {Nhu Hiep}, Pham and {Van Hung}, Nguyen and {Tinh Hien}, Tran and Maia Rabaa and Thwaites, {Guy E.} and Stephen Baker and {Van Tan}, Le and {van Doorn}, {H. Rogier} and Alessandra Berto and Boni, {Maciej F.} and Bryant, {Juliet E.} and Phu, {Bui Duc} and Campbell, {James I.} and Juan Carrique-Mas and Hung, {Dang Manh} and Huong, {Dang Thao} and Oanh, {Dang Tram} and Day, {Jeremy N.} and Tan, {Dinh Van} and Han, {Duong An} and Farrar, {Jeremy J.} and Trang, {Hau Thi Thu} and Long, {Hoang Bao} and Duong, {Hoang Van} and Thu, {Huynh Thi Kim} and Cuong, {Lam Chi} and Hung, {Le Manh} and Phuong, {Le Thanh} and Phuc, {Le Thi} and Phuong, {Le Thi} and Luat, {Le Xuan} and Ha, {Luu Thi Thu} and Chuong, {Ly Van} and Loan, {Mai Thi Phuoc} and Behzad Nadjm and Bao, {Ngo Thanh} and Hoa, {Ngo Thi} and Tue, {Ngo Tri} and Tu, {Nguyen Canh} and Thuan, {Nguyen Dac}",
year = "2017",
month = "1",
day = "1",
doi = "10.12688/wellcomeopenres.12429.1",
language = "English (US)",
volume = "2",
journal = "Wellcome Open Research",
issn = "2398-502X",
publisher = "F1000 Research Ltd.",

}

TY - JOUR

T1 - Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam [version 1; referees

T2 - 2 approved]

AU - VIZIONS consortium

AU - Thi Ty Hang, Vu

AU - Thi Han Ny, Nguyen

AU - My Phuc, Tran

AU - Thi Thanh Tam, Pham

AU - Thao Huong, Dang

AU - Dang Trung Nghia, Ho

AU - Tran Anh Vu, Nguyen

AU - Thi Hong Phuong, Pham

AU - Van Xang, Nguyen

AU - Dong, Nguyen

AU - Nhu Hiep, Pham

AU - Van Hung, Nguyen

AU - Tinh Hien, Tran

AU - Rabaa, Maia

AU - Thwaites, Guy E.

AU - Baker, Stephen

AU - Van Tan, Le

AU - van Doorn, H. Rogier

AU - Berto, Alessandra

AU - Boni, Maciej F.

AU - Bryant, Juliet E.

AU - Phu, Bui Duc

AU - Campbell, James I.

AU - Carrique-Mas, Juan

AU - Hung, Dang Manh

AU - Huong, Dang Thao

AU - Oanh, Dang Tram

AU - Day, Jeremy N.

AU - Tan, Dinh Van

AU - Han, Duong An

AU - Farrar, Jeremy J.

AU - Trang, Hau Thi Thu

AU - Long, Hoang Bao

AU - Duong, Hoang Van

AU - Thu, Huynh Thi Kim

AU - Cuong, Lam Chi

AU - Hung, Le Manh

AU - Phuong, Le Thanh

AU - Phuc, Le Thi

AU - Phuong, Le Thi

AU - Luat, Le Xuan

AU - Ha, Luu Thi Thu

AU - Chuong, Ly Van

AU - Loan, Mai Thi Phuoc

AU - Nadjm, Behzad

AU - Bao, Ngo Thanh

AU - Hoa, Ngo Thi

AU - Tue, Ngo Tri

AU - Tu, Nguyen Canh

AU - Thuan, Nguyen Dac

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Background: Acute respiratory infections (ARI) are among the leading causes of hospitalization in children ≤5 years old. Rapid diagnostics of viral pathogens is essential to avoid unnecessary antibiotic treatment, thereby slowing down antibiotic-resistance. We evaluated the diagnostic performance of the Luminex xTAG Respiratory Viral Panel FAST v2 against viral specific PCR as reference assays for ARI in Vietnam. Methods: Four hundred and forty two nose and throat swabs were collected in viral transport medium, and were tested with Luminex xTAG Respiratory Viral Panel FAST v2. Multiplex RT-PCR and single RT-PCR were used as references. Results: Overall, viral pathogens were detected in a total count of 270/294 (91.8%, 95% CI 88.1-94.7) by the Luminex among reference assays, whilst 112/6336 (1.8%, 95% CI, 1.4-2.1) of pathogens were detected by the Luminex, but not by reference assays. Frequency of pathogens detected by Luminex and reference assays was 379 and 292, respectively. The diagnostic yield was 66.7% (295/442, 95%CI 62.1-71.1%) for the Luminex assay and 54.1% (239/442, 95% CI, 49.3-58.8%) for reference assays. The Luminex kit had higher yields for all viruses except influenza B virus, respiratory syncytial virus, and human bocavirus. High agreements between both methods [mean (range): 0.91 (0.83-1.00)] were found for 10/15 viral agents. Conclusions: The Luminex assay is a high throughput multiplex platform for rapid detection of common viral pathogens causing ARI. Although the current high cost may prevent Luminex assays from being widely used, especially in limited resource settings where ARI are felt most, its introduction in clinical diagnostics may help reduce unnecessary use of antibiotic prescription.

AB - Background: Acute respiratory infections (ARI) are among the leading causes of hospitalization in children ≤5 years old. Rapid diagnostics of viral pathogens is essential to avoid unnecessary antibiotic treatment, thereby slowing down antibiotic-resistance. We evaluated the diagnostic performance of the Luminex xTAG Respiratory Viral Panel FAST v2 against viral specific PCR as reference assays for ARI in Vietnam. Methods: Four hundred and forty two nose and throat swabs were collected in viral transport medium, and were tested with Luminex xTAG Respiratory Viral Panel FAST v2. Multiplex RT-PCR and single RT-PCR were used as references. Results: Overall, viral pathogens were detected in a total count of 270/294 (91.8%, 95% CI 88.1-94.7) by the Luminex among reference assays, whilst 112/6336 (1.8%, 95% CI, 1.4-2.1) of pathogens were detected by the Luminex, but not by reference assays. Frequency of pathogens detected by Luminex and reference assays was 379 and 292, respectively. The diagnostic yield was 66.7% (295/442, 95%CI 62.1-71.1%) for the Luminex assay and 54.1% (239/442, 95% CI, 49.3-58.8%) for reference assays. The Luminex kit had higher yields for all viruses except influenza B virus, respiratory syncytial virus, and human bocavirus. High agreements between both methods [mean (range): 0.91 (0.83-1.00)] were found for 10/15 viral agents. Conclusions: The Luminex assay is a high throughput multiplex platform for rapid detection of common viral pathogens causing ARI. Although the current high cost may prevent Luminex assays from being widely used, especially in limited resource settings where ARI are felt most, its introduction in clinical diagnostics may help reduce unnecessary use of antibiotic prescription.

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U2 - 10.12688/wellcomeopenres.12429.1

DO - 10.12688/wellcomeopenres.12429.1

M3 - Article

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VL - 2

JO - Wellcome Open Research

JF - Wellcome Open Research

SN - 2398-502X

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