Evaluation of tissue PCA3 expression in prostate cancer by RNA in situ hybridization-a correlative study with urine PCA3 and TMPRSS2-ERG

Joshua I. Warrick, Scott A. Tomlins, Shannon L. Carskadon, Allison M. Young, Javed Siddiqui, John T. Wei, Arul M. Chinnaiyan, Lakshmi P. Kunju, Nallasivam Palanisamy

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21 Citations (Scopus)

Abstract

PCA3 is a prostate-specific non-coding RNA, with utility as a urine-based early detection biomarker. Here, we report the evaluation of tissue PCA3 expression by RNA in situ hybridization in a cohort of 41 mapped prostatectomy specimens. We compared tissue PCA3 expression with tissue level ERG expression and matched pre-prostatectomy urine PCA3 and TMPRSS2-ERG levels. Across 136 slides containing 138 foci of prostate cancer, PCA3 was expressed in 55% of cancer foci and 71% of high-grade prostatic intraepithelial neoplasia foci. Overall, the specificity of tissue PCA3 was >90% for prostate cancer and high-grade prostatic intraepithelial neoplasia combined. Tissue PCA3 cancer expression was not significantly associated with urine PCA3 expression. PCA3 and ERG positivity in cancer foci was positively associated (P<0.01). We report the first comprehensive assessment of PCA3 expression in prostatectomy specimens, and find limited correlation between tissue PCA3 and matched urine in prostate cancer.

Original languageEnglish (US)
Pages (from-to)609-620
Number of pages12
JournalModern Pathology
Volume27
Issue number4
DOIs
StatePublished - Apr 2014

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In Situ Hybridization
Prostatic Neoplasms
Urine
RNA
Prostatectomy
Prostatic Intraepithelial Neoplasia
Neoplasms
Untranslated RNA
Organ Specificity
Prostate
Biomarkers

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Warrick, Joshua I. ; Tomlins, Scott A. ; Carskadon, Shannon L. ; Young, Allison M. ; Siddiqui, Javed ; Wei, John T. ; Chinnaiyan, Arul M. ; Kunju, Lakshmi P. ; Palanisamy, Nallasivam. / Evaluation of tissue PCA3 expression in prostate cancer by RNA in situ hybridization-a correlative study with urine PCA3 and TMPRSS2-ERG. In: Modern Pathology. 2014 ; Vol. 27, No. 4. pp. 609-620.
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Warrick, JI, Tomlins, SA, Carskadon, SL, Young, AM, Siddiqui, J, Wei, JT, Chinnaiyan, AM, Kunju, LP & Palanisamy, N 2014, 'Evaluation of tissue PCA3 expression in prostate cancer by RNA in situ hybridization-a correlative study with urine PCA3 and TMPRSS2-ERG', Modern Pathology, vol. 27, no. 4, pp. 609-620. https://doi.org/10.1038/modpathol.2013.169

Evaluation of tissue PCA3 expression in prostate cancer by RNA in situ hybridization-a correlative study with urine PCA3 and TMPRSS2-ERG. / Warrick, Joshua I.; Tomlins, Scott A.; Carskadon, Shannon L.; Young, Allison M.; Siddiqui, Javed; Wei, John T.; Chinnaiyan, Arul M.; Kunju, Lakshmi P.; Palanisamy, Nallasivam.

In: Modern Pathology, Vol. 27, No. 4, 04.2014, p. 609-620.

Research output: Contribution to journalArticle

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AU - Warrick, Joshua I.

AU - Tomlins, Scott A.

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AU - Young, Allison M.

AU - Siddiqui, Javed

AU - Wei, John T.

AU - Chinnaiyan, Arul M.

AU - Kunju, Lakshmi P.

AU - Palanisamy, Nallasivam

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