Evaluation of tissue PCA3 expression in prostate cancer by RNA in situ hybridization-a correlative study with urine PCA3 and TMPRSS2-ERG

Joshua I. Warrick, Scott A. Tomlins, Shannon L. Carskadon, Allison M. Young, Javed Siddiqui, John T. Wei, Arul M. Chinnaiyan, Lakshmi P. Kunju, Nallasivam Palanisamy

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Abstract

PCA3 is a prostate-specific non-coding RNA, with utility as a urine-based early detection biomarker. Here, we report the evaluation of tissue PCA3 expression by RNA in situ hybridization in a cohort of 41 mapped prostatectomy specimens. We compared tissue PCA3 expression with tissue level ERG expression and matched pre-prostatectomy urine PCA3 and TMPRSS2-ERG levels. Across 136 slides containing 138 foci of prostate cancer, PCA3 was expressed in 55% of cancer foci and 71% of high-grade prostatic intraepithelial neoplasia foci. Overall, the specificity of tissue PCA3 was >90% for prostate cancer and high-grade prostatic intraepithelial neoplasia combined. Tissue PCA3 cancer expression was not significantly associated with urine PCA3 expression. PCA3 and ERG positivity in cancer foci was positively associated (P<0.01). We report the first comprehensive assessment of PCA3 expression in prostatectomy specimens, and find limited correlation between tissue PCA3 and matched urine in prostate cancer.

Original languageEnglish (US)
Pages (from-to)609-620
Number of pages12
JournalModern Pathology
Volume27
Issue number4
DOIs
StatePublished - Apr 2014

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

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    Warrick, J. I., Tomlins, S. A., Carskadon, S. L., Young, A. M., Siddiqui, J., Wei, J. T., Chinnaiyan, A. M., Kunju, L. P., & Palanisamy, N. (2014). Evaluation of tissue PCA3 expression in prostate cancer by RNA in situ hybridization-a correlative study with urine PCA3 and TMPRSS2-ERG. Modern Pathology, 27(4), 609-620. https://doi.org/10.1038/modpathol.2013.169