Evaluation of USEPA time-temperature requirement for Escherichia coli destruction by standard culture method and flow cytometry

Wade B. Yankey, Yen Chih Chen, Hongping Chen

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

USEPA time-temperature requirements for fecal coliform destruction were evaluated by both the standard culture method and flow cytometry to investigate if insufficient thermal treatment was the reason for fecal coliforms reactivation observed in a field digester. Results showed that complete inactivation (8-log) of cells can be achieved well below the regulatory requirement. Destruction was found to be permanent where no recovery of viable cell was observed upon further storage up to 2 weeks. Overall, time-temperature treatment is found sufficient to completely inactivate coliform cells but stressors prior to thermal treatment may increase their resistance.

Original languageEnglish (US)
Pages (from-to)9-20
Number of pages12
JournalJournal of Residuals Science and Technology
Volume9
Issue number1
StatePublished - Jan 1 2012

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal

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