Evaluation of USEPA time-temperature requirement for Escherichia coli destruction by standard culture method and flow cytometry

Wade B. Yankey, Yen-chih Chen, Hongping Chen

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

USEPA time-temperature requirements for fecal coliform destruction were evaluated by both the standard culture method and flow cytometry to investigate if insufficient thermal treatment was the reason for fecal coliforms reactivation observed in a field digester. Results showed that complete inactivation (8-log) of cells can be achieved well below the regulatory requirement. Destruction was found to be permanent where no recovery of viable cell was observed upon further storage up to 2 weeks. Overall, time-temperature treatment is found sufficient to completely inactivate coliform cells but stressors prior to thermal treatment may increase their resistance.

Original languageEnglish (US)
Pages (from-to)9-20
Number of pages12
JournalJournal of Residuals Science and Technology
Volume9
Issue number1
StatePublished - Jan 1 2012

Fingerprint

Flow cytometry
flow cytometry
fecal coliform
Escherichia coli
Heat treatment
reactivation
temperature
Recovery
Temperature
evaluation
method
thermal treatment
digester

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal

Cite this

@article{2ac1ee73db9d4d71be8926ee29026964,
title = "Evaluation of USEPA time-temperature requirement for Escherichia coli destruction by standard culture method and flow cytometry",
abstract = "USEPA time-temperature requirements for fecal coliform destruction were evaluated by both the standard culture method and flow cytometry to investigate if insufficient thermal treatment was the reason for fecal coliforms reactivation observed in a field digester. Results showed that complete inactivation (8-log) of cells can be achieved well below the regulatory requirement. Destruction was found to be permanent where no recovery of viable cell was observed upon further storage up to 2 weeks. Overall, time-temperature treatment is found sufficient to completely inactivate coliform cells but stressors prior to thermal treatment may increase their resistance.",
author = "Yankey, {Wade B.} and Yen-chih Chen and Hongping Chen",
year = "2012",
month = "1",
day = "1",
language = "English (US)",
volume = "9",
pages = "9--20",
journal = "Journal of Residuals Science and Technology",
issn = "1544-8053",
publisher = "DEStech Publications Inc.",
number = "1",

}

Evaluation of USEPA time-temperature requirement for Escherichia coli destruction by standard culture method and flow cytometry. / Yankey, Wade B.; Chen, Yen-chih; Chen, Hongping.

In: Journal of Residuals Science and Technology, Vol. 9, No. 1, 01.01.2012, p. 9-20.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Evaluation of USEPA time-temperature requirement for Escherichia coli destruction by standard culture method and flow cytometry

AU - Yankey, Wade B.

AU - Chen, Yen-chih

AU - Chen, Hongping

PY - 2012/1/1

Y1 - 2012/1/1

N2 - USEPA time-temperature requirements for fecal coliform destruction were evaluated by both the standard culture method and flow cytometry to investigate if insufficient thermal treatment was the reason for fecal coliforms reactivation observed in a field digester. Results showed that complete inactivation (8-log) of cells can be achieved well below the regulatory requirement. Destruction was found to be permanent where no recovery of viable cell was observed upon further storage up to 2 weeks. Overall, time-temperature treatment is found sufficient to completely inactivate coliform cells but stressors prior to thermal treatment may increase their resistance.

AB - USEPA time-temperature requirements for fecal coliform destruction were evaluated by both the standard culture method and flow cytometry to investigate if insufficient thermal treatment was the reason for fecal coliforms reactivation observed in a field digester. Results showed that complete inactivation (8-log) of cells can be achieved well below the regulatory requirement. Destruction was found to be permanent where no recovery of viable cell was observed upon further storage up to 2 weeks. Overall, time-temperature treatment is found sufficient to completely inactivate coliform cells but stressors prior to thermal treatment may increase their resistance.

UR - http://www.scopus.com/inward/record.url?scp=84863354570&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863354570&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84863354570

VL - 9

SP - 9

EP - 20

JO - Journal of Residuals Science and Technology

JF - Journal of Residuals Science and Technology

SN - 1544-8053

IS - 1

ER -