Evidence against the involvement of ionically bound cell wall proteins in pea epicotyl growth.

M. A. Melan, D. J. Cosgrove

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Ionically bound cell wall proteins were extracted from 7 day old etiolated pea (Pisum sativum L. cv Alaska) epicotyls with 3 molar LiCl. Polyclonal antiserum was raised in rabbits against the cell wall proteins. Growth assays showed that treatment of growing region segments (5-7 millimeters) of peas with either dialyzed serum, serum globulin fraction, affinity purified immunoglobulin, or papain-cleaved antibody fragments had no effect on growth. Immunofluorescence microscopy confirmed antibody binding to cell walls and penetration of the antibodies into the tissues. Western blot analysis, immunoassay results, and affinity chromatography utilizing Sepharose-bound antibodies confirmed recognition of the protein preparation by the antibodies. Experiments employing in vitro extension as a screening measure indicated no effect upon extension by antibodies, by 50 millimolar LiCl perfusion of the apoplast or by 3 molar LiCl extraction. Addition of cell wall protein to protease pretreated segments did not restore extension nor did addition of cell wall protein to untreated segments increase extension. It is concluded that, although evidence suggests that protein is responsible for the process of extension, the class(es) of proteins which are extracted from pea cell walls with 3 molar LiCl are probably not involved in this process.

Original languageEnglish (US)
Pages (from-to)469-474
Number of pages6
JournalPlant physiology
Volume86
DOIs
StatePublished - 1988

Fingerprint

pea protein
epicotyls
Peas
Cell Wall
cell walls
Growth
antibodies
Antibodies
Proteins
proteins
peas
blood serum
Serum Globulins
Immunoglobulin Fragments
Papain
papain
apoplast
affinity chromatography
fluorescence microscopy
immunoassays

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

@article{087b16337da44ab4a33868920da2516e,
title = "Evidence against the involvement of ionically bound cell wall proteins in pea epicotyl growth.",
abstract = "Ionically bound cell wall proteins were extracted from 7 day old etiolated pea (Pisum sativum L. cv Alaska) epicotyls with 3 molar LiCl. Polyclonal antiserum was raised in rabbits against the cell wall proteins. Growth assays showed that treatment of growing region segments (5-7 millimeters) of peas with either dialyzed serum, serum globulin fraction, affinity purified immunoglobulin, or papain-cleaved antibody fragments had no effect on growth. Immunofluorescence microscopy confirmed antibody binding to cell walls and penetration of the antibodies into the tissues. Western blot analysis, immunoassay results, and affinity chromatography utilizing Sepharose-bound antibodies confirmed recognition of the protein preparation by the antibodies. Experiments employing in vitro extension as a screening measure indicated no effect upon extension by antibodies, by 50 millimolar LiCl perfusion of the apoplast or by 3 molar LiCl extraction. Addition of cell wall protein to protease pretreated segments did not restore extension nor did addition of cell wall protein to untreated segments increase extension. It is concluded that, although evidence suggests that protein is responsible for the process of extension, the class(es) of proteins which are extracted from pea cell walls with 3 molar LiCl are probably not involved in this process.",
author = "Melan, {M. A.} and Cosgrove, {D. J.}",
year = "1988",
doi = "10.1104/pp.86.2.469",
language = "English (US)",
volume = "86",
pages = "469--474",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",

}

Evidence against the involvement of ionically bound cell wall proteins in pea epicotyl growth. / Melan, M. A.; Cosgrove, D. J.

In: Plant physiology, Vol. 86, 1988, p. 469-474.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Evidence against the involvement of ionically bound cell wall proteins in pea epicotyl growth.

AU - Melan, M. A.

AU - Cosgrove, D. J.

PY - 1988

Y1 - 1988

N2 - Ionically bound cell wall proteins were extracted from 7 day old etiolated pea (Pisum sativum L. cv Alaska) epicotyls with 3 molar LiCl. Polyclonal antiserum was raised in rabbits against the cell wall proteins. Growth assays showed that treatment of growing region segments (5-7 millimeters) of peas with either dialyzed serum, serum globulin fraction, affinity purified immunoglobulin, or papain-cleaved antibody fragments had no effect on growth. Immunofluorescence microscopy confirmed antibody binding to cell walls and penetration of the antibodies into the tissues. Western blot analysis, immunoassay results, and affinity chromatography utilizing Sepharose-bound antibodies confirmed recognition of the protein preparation by the antibodies. Experiments employing in vitro extension as a screening measure indicated no effect upon extension by antibodies, by 50 millimolar LiCl perfusion of the apoplast or by 3 molar LiCl extraction. Addition of cell wall protein to protease pretreated segments did not restore extension nor did addition of cell wall protein to untreated segments increase extension. It is concluded that, although evidence suggests that protein is responsible for the process of extension, the class(es) of proteins which are extracted from pea cell walls with 3 molar LiCl are probably not involved in this process.

AB - Ionically bound cell wall proteins were extracted from 7 day old etiolated pea (Pisum sativum L. cv Alaska) epicotyls with 3 molar LiCl. Polyclonal antiserum was raised in rabbits against the cell wall proteins. Growth assays showed that treatment of growing region segments (5-7 millimeters) of peas with either dialyzed serum, serum globulin fraction, affinity purified immunoglobulin, or papain-cleaved antibody fragments had no effect on growth. Immunofluorescence microscopy confirmed antibody binding to cell walls and penetration of the antibodies into the tissues. Western blot analysis, immunoassay results, and affinity chromatography utilizing Sepharose-bound antibodies confirmed recognition of the protein preparation by the antibodies. Experiments employing in vitro extension as a screening measure indicated no effect upon extension by antibodies, by 50 millimolar LiCl perfusion of the apoplast or by 3 molar LiCl extraction. Addition of cell wall protein to protease pretreated segments did not restore extension nor did addition of cell wall protein to untreated segments increase extension. It is concluded that, although evidence suggests that protein is responsible for the process of extension, the class(es) of proteins which are extracted from pea cell walls with 3 molar LiCl are probably not involved in this process.

UR - http://www.scopus.com/inward/record.url?scp=0024275230&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024275230&partnerID=8YFLogxK

U2 - 10.1104/pp.86.2.469

DO - 10.1104/pp.86.2.469

M3 - Article

C2 - 11538235

AN - SCOPUS:0024275230

VL - 86

SP - 469

EP - 474

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

ER -