Evidence for an unusual transmembrane configuration of AGG3, a class C Gγ subunit of Arabidopsis

Susanne Wolfenstetter, David Chakravorty, Ryan Kula, Daisuke Urano, Yuri Trusov, Michael B. Sheahan, David W. McCurdy, Sarah M. Assmann, Alan M. Jones, José R. Botella

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Heterotrimeric G proteins are crucial for the perception of external signals and subsequent signal transduction in animal and plant cells. In both model systems, the complex comprises one Gα, one Gβ, and one Gγ subunit. However, in addition to the canonical Gγ subunits (class A), plants also possess two unusual, plant-specific classes of Gγ subunits (classes B and C) that have not yet been found in animals. These include Gγ subunits lacking the C-terminal CaaX motif (class B), which is important for membrane anchoring of the protein; the presence of such subunits gives rise to a flexible sub-population of Gβ/γ heterodimers that are not necessarily restricted to the plasma membrane. Plants also contain class C Gγ subunits, which are twice the size of canonical Gγ subunits, with a predicted transmembrane domain and a large cysteine-rich extracellular C-terminus. However, neither the presence of the transmembrane domain nor the membrane topology have been unequivocally demonstrated. Here, we provide compelling evidence that AGG3, a class C Gγ subunit of Arabidopsis, contains a functional transmembrane domain, which is sufficient but not essential for plasma membrane localization, and that the cysteine-rich C-terminus is extracellular. Significance Statement AGG3 is the prototype of an unusual Gγ subunit of the heterotrimeric G protein complex. It is a type II membrane protein having a cysteine-rich extracellular domain.

Original languageEnglish (US)
Pages (from-to)388-398
Number of pages11
JournalPlant Journal
Volume81
Issue number3
DOIs
StatePublished - Feb 1 2015

Fingerprint

Arabidopsis
Cysteine
Heterotrimeric GTP-Binding Proteins
cysteine
Membrane Proteins
G-proteins
Cell Membrane
membrane proteins
plasma membrane
Plant Cells
Signal Transduction
prototypes
topology
signal transduction
animals
Membranes
Population
cells

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Wolfenstetter, S., Chakravorty, D., Kula, R., Urano, D., Trusov, Y., Sheahan, M. B., ... Botella, J. R. (2015). Evidence for an unusual transmembrane configuration of AGG3, a class C Gγ subunit of Arabidopsis. Plant Journal, 81(3), 388-398. https://doi.org/10.1111/tpj.12732
Wolfenstetter, Susanne ; Chakravorty, David ; Kula, Ryan ; Urano, Daisuke ; Trusov, Yuri ; Sheahan, Michael B. ; McCurdy, David W. ; Assmann, Sarah M. ; Jones, Alan M. ; Botella, José R. / Evidence for an unusual transmembrane configuration of AGG3, a class C Gγ subunit of Arabidopsis. In: Plant Journal. 2015 ; Vol. 81, No. 3. pp. 388-398.
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Wolfenstetter, S, Chakravorty, D, Kula, R, Urano, D, Trusov, Y, Sheahan, MB, McCurdy, DW, Assmann, SM, Jones, AM & Botella, JR 2015, 'Evidence for an unusual transmembrane configuration of AGG3, a class C Gγ subunit of Arabidopsis', Plant Journal, vol. 81, no. 3, pp. 388-398. https://doi.org/10.1111/tpj.12732

Evidence for an unusual transmembrane configuration of AGG3, a class C Gγ subunit of Arabidopsis. / Wolfenstetter, Susanne; Chakravorty, David; Kula, Ryan; Urano, Daisuke; Trusov, Yuri; Sheahan, Michael B.; McCurdy, David W.; Assmann, Sarah M.; Jones, Alan M.; Botella, José R.

In: Plant Journal, Vol. 81, No. 3, 01.02.2015, p. 388-398.

Research output: Contribution to journalArticle

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AU - Wolfenstetter, Susanne

AU - Chakravorty, David

AU - Kula, Ryan

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