Evidence for clonal spread of a single strain of β-lactamase-producing Enterococcus (Streptococcus) faecalis to six hospitals in five states

B. E. Murray, K. V. Singh, S. M. Markowitz, H. A. Lopardo, J. E. Patterson, M. J. Zervos, E. Rubeglio, G. M. Eliopoulos, L. B. Rice, F. W. Goldstein, S. G. Jenkins, G. M. Caputo, R. Nasnas, L. S. Moore, E. S. Wong, G. Weinstock

Research output: Contribution to journalArticle

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Abstract

β-lactamase-producing (Bla+) enterococci have been reported in three states and two countries. Pulsed-field gel electrophoresis was used to compare 14 Bla+ Enterococcus (Streptococcus) faecalis isolated from hospitalized patients in seven states and three continents. The restriction endonuclease digestion patterns of isolates from Connecticut, Massachusetts, Lebanon, and Argentina were all markedly different, indicating that these were different strains. However, isolates from Delaware, Texas, Pennsylvania (Philadelphia and Pittsburgh), Florida, and Virginia were similar, indicating that these isolates were derivatives of a single strain. This conclusion was supported by hybridization using individual fragments as probes. Spread of Bla+ enterococci within the hospital setting was also demonstrated. These findings illustrate the value of pulsed-field gel electrophoresis for epidemiologic analyses and support the importance of identifying and containing organisms with new resistance properties in an effort to decrease their transmission to and from, as well as within, hospitals.

Original languageEnglish (US)
Pages (from-to)780-785
Number of pages6
JournalJournal of Infectious Diseases
Volume163
Issue number4
DOIs
StatePublished - Jan 1 1991

Fingerprint

Enterococcus faecalis
Enterococcus
Pulsed Field Gel Electrophoresis
Lebanon
Argentina
DNA Restriction Enzymes
Digestion

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Murray, B. E., Singh, K. V., Markowitz, S. M., Lopardo, H. A., Patterson, J. E., Zervos, M. J., ... Weinstock, G. (1991). Evidence for clonal spread of a single strain of β-lactamase-producing Enterococcus (Streptococcus) faecalis to six hospitals in five states. Journal of Infectious Diseases, 163(4), 780-785. https://doi.org/10.1093/infdis/163.4.780
Murray, B. E. ; Singh, K. V. ; Markowitz, S. M. ; Lopardo, H. A. ; Patterson, J. E. ; Zervos, M. J. ; Rubeglio, E. ; Eliopoulos, G. M. ; Rice, L. B. ; Goldstein, F. W. ; Jenkins, S. G. ; Caputo, G. M. ; Nasnas, R. ; Moore, L. S. ; Wong, E. S. ; Weinstock, G. / Evidence for clonal spread of a single strain of β-lactamase-producing Enterococcus (Streptococcus) faecalis to six hospitals in five states. In: Journal of Infectious Diseases. 1991 ; Vol. 163, No. 4. pp. 780-785.
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abstract = "β-lactamase-producing (Bla+) enterococci have been reported in three states and two countries. Pulsed-field gel electrophoresis was used to compare 14 Bla+ Enterococcus (Streptococcus) faecalis isolated from hospitalized patients in seven states and three continents. The restriction endonuclease digestion patterns of isolates from Connecticut, Massachusetts, Lebanon, and Argentina were all markedly different, indicating that these were different strains. However, isolates from Delaware, Texas, Pennsylvania (Philadelphia and Pittsburgh), Florida, and Virginia were similar, indicating that these isolates were derivatives of a single strain. This conclusion was supported by hybridization using individual fragments as probes. Spread of Bla+ enterococci within the hospital setting was also demonstrated. These findings illustrate the value of pulsed-field gel electrophoresis for epidemiologic analyses and support the importance of identifying and containing organisms with new resistance properties in an effort to decrease their transmission to and from, as well as within, hospitals.",
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Murray, BE, Singh, KV, Markowitz, SM, Lopardo, HA, Patterson, JE, Zervos, MJ, Rubeglio, E, Eliopoulos, GM, Rice, LB, Goldstein, FW, Jenkins, SG, Caputo, GM, Nasnas, R, Moore, LS, Wong, ES & Weinstock, G 1991, 'Evidence for clonal spread of a single strain of β-lactamase-producing Enterococcus (Streptococcus) faecalis to six hospitals in five states', Journal of Infectious Diseases, vol. 163, no. 4, pp. 780-785. https://doi.org/10.1093/infdis/163.4.780

Evidence for clonal spread of a single strain of β-lactamase-producing Enterococcus (Streptococcus) faecalis to six hospitals in five states. / Murray, B. E.; Singh, K. V.; Markowitz, S. M.; Lopardo, H. A.; Patterson, J. E.; Zervos, M. J.; Rubeglio, E.; Eliopoulos, G. M.; Rice, L. B.; Goldstein, F. W.; Jenkins, S. G.; Caputo, G. M.; Nasnas, R.; Moore, L. S.; Wong, E. S.; Weinstock, G.

In: Journal of Infectious Diseases, Vol. 163, No. 4, 01.01.1991, p. 780-785.

Research output: Contribution to journalArticle

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T1 - Evidence for clonal spread of a single strain of β-lactamase-producing Enterococcus (Streptococcus) faecalis to six hospitals in five states

AU - Murray, B. E.

AU - Singh, K. V.

AU - Markowitz, S. M.

AU - Lopardo, H. A.

AU - Patterson, J. E.

AU - Zervos, M. J.

AU - Rubeglio, E.

AU - Eliopoulos, G. M.

AU - Rice, L. B.

AU - Goldstein, F. W.

AU - Jenkins, S. G.

AU - Caputo, G. M.

AU - Nasnas, R.

AU - Moore, L. S.

AU - Wong, E. S.

AU - Weinstock, G.

PY - 1991/1/1

Y1 - 1991/1/1

N2 - β-lactamase-producing (Bla+) enterococci have been reported in three states and two countries. Pulsed-field gel electrophoresis was used to compare 14 Bla+ Enterococcus (Streptococcus) faecalis isolated from hospitalized patients in seven states and three continents. The restriction endonuclease digestion patterns of isolates from Connecticut, Massachusetts, Lebanon, and Argentina were all markedly different, indicating that these were different strains. However, isolates from Delaware, Texas, Pennsylvania (Philadelphia and Pittsburgh), Florida, and Virginia were similar, indicating that these isolates were derivatives of a single strain. This conclusion was supported by hybridization using individual fragments as probes. Spread of Bla+ enterococci within the hospital setting was also demonstrated. These findings illustrate the value of pulsed-field gel electrophoresis for epidemiologic analyses and support the importance of identifying and containing organisms with new resistance properties in an effort to decrease their transmission to and from, as well as within, hospitals.

AB - β-lactamase-producing (Bla+) enterococci have been reported in three states and two countries. Pulsed-field gel electrophoresis was used to compare 14 Bla+ Enterococcus (Streptococcus) faecalis isolated from hospitalized patients in seven states and three continents. The restriction endonuclease digestion patterns of isolates from Connecticut, Massachusetts, Lebanon, and Argentina were all markedly different, indicating that these were different strains. However, isolates from Delaware, Texas, Pennsylvania (Philadelphia and Pittsburgh), Florida, and Virginia were similar, indicating that these isolates were derivatives of a single strain. This conclusion was supported by hybridization using individual fragments as probes. Spread of Bla+ enterococci within the hospital setting was also demonstrated. These findings illustrate the value of pulsed-field gel electrophoresis for epidemiologic analyses and support the importance of identifying and containing organisms with new resistance properties in an effort to decrease their transmission to and from, as well as within, hospitals.

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