Abstract
Core extrusion of the bound iron-sulfur centers from spinach Photosystem I showed the presence of [2Fe-2S] clusters as well as [4Fe-4S] clusters among FA, FB and FX. Extrusion of the iron-sulfur ensemble was not quantitative; however, the presence of [2Fe-2S] clusters correlated with higher concentration of unfolding solvent. Since FX is highly resistant to denaturation, and since FA and FB are known to contain [4Fe-4S] clusters, the [2Fe-2S] clusters are assigned to FX. The presence of [2Fe-2S] clusters in Photosystem I has significance in the structure and organization of FX on the reaction center. Since four cysteinyl ligands are assumed to hold an iron-sulfur cluster, a Photosystem I subunit may consist of two approx. 64-kDa proteins bridged by a single [2Fe-2S] cluster. The complete reaction center would consist of two subunits positioned so that two [2Fe-2S] clusters are in magnetic interaction, thereby constituting FX.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 94-98 |
| Number of pages | 5 |
| Journal | BBA - Bioenergetics |
| Volume | 891 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 25 1987 |
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All Science Journal Classification (ASJC) codes
- Biophysics
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Evidence for the existence of [2Fe-2S] as well as [4Fe-4S] clusters among FA, FB and FX. Implications for the structure of the Photosystem I reaction center. / Golbeck, John H.; McDermott, Ann E.; Jones, Wayne K.; Kurtz, D. M.
In: BBA - Bioenergetics, Vol. 891, No. 1, 25.03.1987, p. 94-98.Research output: Contribution to journal › Article
TY - JOUR
T1 - Evidence for the existence of [2Fe-2S] as well as [4Fe-4S] clusters among FA, FB and FX. Implications for the structure of the Photosystem I reaction center
AU - Golbeck, John H.
AU - McDermott, Ann E.
AU - Jones, Wayne K.
AU - Kurtz, D. M.
PY - 1987/3/25
Y1 - 1987/3/25
N2 - Core extrusion of the bound iron-sulfur centers from spinach Photosystem I showed the presence of [2Fe-2S] clusters as well as [4Fe-4S] clusters among FA, FB and FX. Extrusion of the iron-sulfur ensemble was not quantitative; however, the presence of [2Fe-2S] clusters correlated with higher concentration of unfolding solvent. Since FX is highly resistant to denaturation, and since FA and FB are known to contain [4Fe-4S] clusters, the [2Fe-2S] clusters are assigned to FX. The presence of [2Fe-2S] clusters in Photosystem I has significance in the structure and organization of FX on the reaction center. Since four cysteinyl ligands are assumed to hold an iron-sulfur cluster, a Photosystem I subunit may consist of two approx. 64-kDa proteins bridged by a single [2Fe-2S] cluster. The complete reaction center would consist of two subunits positioned so that two [2Fe-2S] clusters are in magnetic interaction, thereby constituting FX.
AB - Core extrusion of the bound iron-sulfur centers from spinach Photosystem I showed the presence of [2Fe-2S] clusters as well as [4Fe-4S] clusters among FA, FB and FX. Extrusion of the iron-sulfur ensemble was not quantitative; however, the presence of [2Fe-2S] clusters correlated with higher concentration of unfolding solvent. Since FX is highly resistant to denaturation, and since FA and FB are known to contain [4Fe-4S] clusters, the [2Fe-2S] clusters are assigned to FX. The presence of [2Fe-2S] clusters in Photosystem I has significance in the structure and organization of FX on the reaction center. Since four cysteinyl ligands are assumed to hold an iron-sulfur cluster, a Photosystem I subunit may consist of two approx. 64-kDa proteins bridged by a single [2Fe-2S] cluster. The complete reaction center would consist of two subunits positioned so that two [2Fe-2S] clusters are in magnetic interaction, thereby constituting FX.
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U2 - 10.1016/0005-2728(87)90087-9
DO - 10.1016/0005-2728(87)90087-9
M3 - Article
VL - 891
SP - 94
EP - 98
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
SN - 0005-2728
IS - 1
ER -