Evidence for the existence of [2Fe-2S] as well as [4Fe-4S] clusters among FA, FB and FX. Implications for the structure of the Photosystem I reaction center

John H. Golbeck, Ann E. McDermott, Wayne K. Jones, D. M. Kurtz

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Core extrusion of the bound iron-sulfur centers from spinach Photosystem I showed the presence of [2Fe-2S] clusters as well as [4Fe-4S] clusters among FA, FB and FX. Extrusion of the iron-sulfur ensemble was not quantitative; however, the presence of [2Fe-2S] clusters correlated with higher concentration of unfolding solvent. Since FX is highly resistant to denaturation, and since FA and FB are known to contain [4Fe-4S] clusters, the [2Fe-2S] clusters are assigned to FX. The presence of [2Fe-2S] clusters in Photosystem I has significance in the structure and organization of FX on the reaction center. Since four cysteinyl ligands are assumed to hold an iron-sulfur cluster, a Photosystem I subunit may consist of two approx. 64-kDa proteins bridged by a single [2Fe-2S] cluster. The complete reaction center would consist of two subunits positioned so that two [2Fe-2S] clusters are in magnetic interaction, thereby constituting FX.

Original languageEnglish (US)
Pages (from-to)94-98
Number of pages5
JournalBBA - Bioenergetics
Volume891
Issue number1
DOIs
StatePublished - Mar 25 1987

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Photosystem I Protein Complex
Sulfur
Iron
Extrusion
Denaturation
Spinacia oleracea
Ligands
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics

Cite this

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abstract = "Core extrusion of the bound iron-sulfur centers from spinach Photosystem I showed the presence of [2Fe-2S] clusters as well as [4Fe-4S] clusters among FA, FB and FX. Extrusion of the iron-sulfur ensemble was not quantitative; however, the presence of [2Fe-2S] clusters correlated with higher concentration of unfolding solvent. Since FX is highly resistant to denaturation, and since FA and FB are known to contain [4Fe-4S] clusters, the [2Fe-2S] clusters are assigned to FX. The presence of [2Fe-2S] clusters in Photosystem I has significance in the structure and organization of FX on the reaction center. Since four cysteinyl ligands are assumed to hold an iron-sulfur cluster, a Photosystem I subunit may consist of two approx. 64-kDa proteins bridged by a single [2Fe-2S] cluster. The complete reaction center would consist of two subunits positioned so that two [2Fe-2S] clusters are in magnetic interaction, thereby constituting FX.",
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Evidence for the existence of [2Fe-2S] as well as [4Fe-4S] clusters among FA, FB and FX. Implications for the structure of the Photosystem I reaction center. / Golbeck, John H.; McDermott, Ann E.; Jones, Wayne K.; Kurtz, D. M.

In: BBA - Bioenergetics, Vol. 891, No. 1, 25.03.1987, p. 94-98.

Research output: Contribution to journalArticle

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