Evidence supporting a Zoonotic Origin of human coronavirus strain NL63

Jeremy Huynh, Shimena Li, Boyd Yount, Alexander Smith, Leslie Sturges, John C. Olsen, Juliet Nagel, Joshua B. Johnson, Sudhakar Agnihothram, J. Edward Gates, Matthew B. Frieman, Ralph S. Baric, Eric F. Donaldson

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

The relationship between bats and coronaviruses (CoVs) has received considerable attention since the severe acute respiratory syndrome (SARS)-like CoV was identified in the Chinese horseshoe bat (Rhinolophidae) in 2005. Since then, several bats throughout the world have been shown to shed CoV sequences, and presumably CoVs, in the feces; however, no bat CoVs have been isolated from nature. Moreover, there are very few bat cell lines or reagents available for investigating CoV replication in bat cells or for isolating bat CoVs adapted to specific bat species. Here, we show by molecular clock analysis that alphacoronavirus (α-CoV) sequences derived from the North American tricolored bat (Perimyotis subflavus) are predicted to share common ancestry with human CoV (HCoV)-NL63, with the most recent common ancestor between these viruses occurring approximately 563 to 822 years ago. Further, we developed immortalized bat cell lines from the lungs of this bat species to determine if these cells were capable of supporting infection with HCoVs. While SARS-CoV, mouse-adapted SARS-CoV (MA15), and chimeric SARS-CoVs bearing the spike genes of early human strains replicated inefficiently, HCoV-NL63 replicated for multiple passages in the immortalized lung cells from this bat species. These observations support the hypothesis that human CoVs are capable of establishing zoonotic-reverse zoonotic transmission cycles that may allow some CoVs to readily circulate and exchange genetic material between strains found in bats and other mammals, including humans.

Original languageEnglish (US)
Pages (from-to)12816-12825
Number of pages10
JournalJournal of virology
Volume86
Issue number23
DOIs
StatePublished - Dec 1 2012

Fingerprint

Human Coronavirus NL63
Coronavirinae
Zoonoses
Chiroptera
Coronavirus
Severe Acute Respiratory Syndrome
Alphacoronavirus
ancestry
lungs
Rhinolophidae
cell lines
Cell Line
Lung

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Huynh, J., Li, S., Yount, B., Smith, A., Sturges, L., Olsen, J. C., ... Donaldson, E. F. (2012). Evidence supporting a Zoonotic Origin of human coronavirus strain NL63. Journal of virology, 86(23), 12816-12825. https://doi.org/10.1128/JVI.00906-12
Huynh, Jeremy ; Li, Shimena ; Yount, Boyd ; Smith, Alexander ; Sturges, Leslie ; Olsen, John C. ; Nagel, Juliet ; Johnson, Joshua B. ; Agnihothram, Sudhakar ; Gates, J. Edward ; Frieman, Matthew B. ; Baric, Ralph S. ; Donaldson, Eric F. / Evidence supporting a Zoonotic Origin of human coronavirus strain NL63. In: Journal of virology. 2012 ; Vol. 86, No. 23. pp. 12816-12825.
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Huynh, J, Li, S, Yount, B, Smith, A, Sturges, L, Olsen, JC, Nagel, J, Johnson, JB, Agnihothram, S, Gates, JE, Frieman, MB, Baric, RS & Donaldson, EF 2012, 'Evidence supporting a Zoonotic Origin of human coronavirus strain NL63', Journal of virology, vol. 86, no. 23, pp. 12816-12825. https://doi.org/10.1128/JVI.00906-12

Evidence supporting a Zoonotic Origin of human coronavirus strain NL63. / Huynh, Jeremy; Li, Shimena; Yount, Boyd; Smith, Alexander; Sturges, Leslie; Olsen, John C.; Nagel, Juliet; Johnson, Joshua B.; Agnihothram, Sudhakar; Gates, J. Edward; Frieman, Matthew B.; Baric, Ralph S.; Donaldson, Eric F.

In: Journal of virology, Vol. 86, No. 23, 01.12.2012, p. 12816-12825.

Research output: Contribution to journalArticle

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AU - Li, Shimena

AU - Yount, Boyd

AU - Smith, Alexander

AU - Sturges, Leslie

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AU - Nagel, Juliet

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AU - Agnihothram, Sudhakar

AU - Gates, J. Edward

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AU - Baric, Ralph S.

AU - Donaldson, Eric F.

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Huynh J, Li S, Yount B, Smith A, Sturges L, Olsen JC et al. Evidence supporting a Zoonotic Origin of human coronavirus strain NL63. Journal of virology. 2012 Dec 1;86(23):12816-12825. https://doi.org/10.1128/JVI.00906-12