Evidence that prenatal programming of hypertension by dietary protein deprivation is mediated by fetal glucocorticoid exposure

Sabeen Habib, Jyothsna Gattineni, Katherine Twombley, Michel Baum

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Background Prenatal programming by maternal dietary protein deprivation and prenatal dexamethasone result in a reduction in nephron number and hypertension when the offspring are studied as adults. Methods To determine whether prenatal dietary protein deprivation results in a reduction in nephron number and hypertension in offspring by exposure to maternal glucocorticoids, we administered metyrapone to rats fed either a 6% oR20% protein diet to inhibit glucocorticoid production and compared the offspring to rats that were the product of mothers fed either a 6% oR20% protein diet during the last half of pregnancy. Results Male offspring from the 6% group had elevated systolic blood pressure (149±2 vs. 130±5mmHg, P<0.05) and a reduction in glomeruli compared to the 20% group (22,111±627 vs. 29,666±654 glomeruli/kidney, P<0.001). Maternal metyrapone administration did not affect the blood pressure in the 20% group but ameliorated the increase in blood pressure in the 6% male group to values comparable to the 20% control group (138±6 vs. 130±5mmHg). Male offspring of the 6% group that received metyrapone had an increase in the number of glomeruli compared to the vehicle-treated 6% group (26,780±377 vs. 22,111±627 glomeruli/kidney, P<0.001), but less glomeruli compared to the 20% protein control group (26,780±377 vs. 29,666±654 glomeruli/kidney, P <0.01). Conclusions The reduction in nephron number and hypertension induced by maternal protein deprivation in male offspring is ameliorated by inhibition of glucocorticoid production.

Original languageEnglish (US)
Pages (from-to)96-101
Number of pages6
JournalAmerican journal of hypertension
Volume24
Issue number1
DOIs
StatePublished - Jan 1 2011

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Embryonic and Fetal Development
Dietary Proteins
Kidney Glomerulus
Metyrapone
Glucocorticoids
Nephrons
Blood Pressure
Hypertension
Mothers
Proteins
Maternal Deprivation
Diet
Maternal Exposure
Control Groups
Dexamethasone
Pregnancy

All Science Journal Classification (ASJC) codes

  • Internal Medicine

Cite this

Habib, Sabeen ; Gattineni, Jyothsna ; Twombley, Katherine ; Baum, Michel. / Evidence that prenatal programming of hypertension by dietary protein deprivation is mediated by fetal glucocorticoid exposure. In: American journal of hypertension. 2011 ; Vol. 24, No. 1. pp. 96-101.
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abstract = "Background Prenatal programming by maternal dietary protein deprivation and prenatal dexamethasone result in a reduction in nephron number and hypertension when the offspring are studied as adults. Methods To determine whether prenatal dietary protein deprivation results in a reduction in nephron number and hypertension in offspring by exposure to maternal glucocorticoids, we administered metyrapone to rats fed either a 6{\%} oR20{\%} protein diet to inhibit glucocorticoid production and compared the offspring to rats that were the product of mothers fed either a 6{\%} oR20{\%} protein diet during the last half of pregnancy. Results Male offspring from the 6{\%} group had elevated systolic blood pressure (149±2 vs. 130±5mmHg, P<0.05) and a reduction in glomeruli compared to the 20{\%} group (22,111±627 vs. 29,666±654 glomeruli/kidney, P<0.001). Maternal metyrapone administration did not affect the blood pressure in the 20{\%} group but ameliorated the increase in blood pressure in the 6{\%} male group to values comparable to the 20{\%} control group (138±6 vs. 130±5mmHg). Male offspring of the 6{\%} group that received metyrapone had an increase in the number of glomeruli compared to the vehicle-treated 6{\%} group (26,780±377 vs. 22,111±627 glomeruli/kidney, P<0.001), but less glomeruli compared to the 20{\%} protein control group (26,780±377 vs. 29,666±654 glomeruli/kidney, P <0.01). Conclusions The reduction in nephron number and hypertension induced by maternal protein deprivation in male offspring is ameliorated by inhibition of glucocorticoid production.",
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Evidence that prenatal programming of hypertension by dietary protein deprivation is mediated by fetal glucocorticoid exposure. / Habib, Sabeen; Gattineni, Jyothsna; Twombley, Katherine; Baum, Michel.

In: American journal of hypertension, Vol. 24, No. 1, 01.01.2011, p. 96-101.

Research output: Contribution to journalArticle

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AU - Twombley, Katherine

AU - Baum, Michel

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N2 - Background Prenatal programming by maternal dietary protein deprivation and prenatal dexamethasone result in a reduction in nephron number and hypertension when the offspring are studied as adults. Methods To determine whether prenatal dietary protein deprivation results in a reduction in nephron number and hypertension in offspring by exposure to maternal glucocorticoids, we administered metyrapone to rats fed either a 6% oR20% protein diet to inhibit glucocorticoid production and compared the offspring to rats that were the product of mothers fed either a 6% oR20% protein diet during the last half of pregnancy. Results Male offspring from the 6% group had elevated systolic blood pressure (149±2 vs. 130±5mmHg, P<0.05) and a reduction in glomeruli compared to the 20% group (22,111±627 vs. 29,666±654 glomeruli/kidney, P<0.001). Maternal metyrapone administration did not affect the blood pressure in the 20% group but ameliorated the increase in blood pressure in the 6% male group to values comparable to the 20% control group (138±6 vs. 130±5mmHg). Male offspring of the 6% group that received metyrapone had an increase in the number of glomeruli compared to the vehicle-treated 6% group (26,780±377 vs. 22,111±627 glomeruli/kidney, P<0.001), but less glomeruli compared to the 20% protein control group (26,780±377 vs. 29,666±654 glomeruli/kidney, P <0.01). Conclusions The reduction in nephron number and hypertension induced by maternal protein deprivation in male offspring is ameliorated by inhibition of glucocorticoid production.

AB - Background Prenatal programming by maternal dietary protein deprivation and prenatal dexamethasone result in a reduction in nephron number and hypertension when the offspring are studied as adults. Methods To determine whether prenatal dietary protein deprivation results in a reduction in nephron number and hypertension in offspring by exposure to maternal glucocorticoids, we administered metyrapone to rats fed either a 6% oR20% protein diet to inhibit glucocorticoid production and compared the offspring to rats that were the product of mothers fed either a 6% oR20% protein diet during the last half of pregnancy. Results Male offspring from the 6% group had elevated systolic blood pressure (149±2 vs. 130±5mmHg, P<0.05) and a reduction in glomeruli compared to the 20% group (22,111±627 vs. 29,666±654 glomeruli/kidney, P<0.001). Maternal metyrapone administration did not affect the blood pressure in the 20% group but ameliorated the increase in blood pressure in the 6% male group to values comparable to the 20% control group (138±6 vs. 130±5mmHg). Male offspring of the 6% group that received metyrapone had an increase in the number of glomeruli compared to the vehicle-treated 6% group (26,780±377 vs. 22,111±627 glomeruli/kidney, P<0.001), but less glomeruli compared to the 20% protein control group (26,780±377 vs. 29,666±654 glomeruli/kidney, P <0.01). Conclusions The reduction in nephron number and hypertension induced by maternal protein deprivation in male offspring is ameliorated by inhibition of glucocorticoid production.

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