Evolution and organization of a highly dynamic, subtelomeric helicase gene family in the rice blast fungus Magnaporthe grisea

Weimin Gao, Chang Hyun Khang, Sook Young Park, Yong Hwan Lee, Seogchan Kang

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Sequence analysis of a 13-kb telomeric region in O-137, a rice pathogenic isolate of Magnaporthe grisea, uncovered a novel gene, designated TLH1 (telomere-linked helicase 1). The TLH1 gene is a member of a gene family, and the sequences flanking this gene family have also been amplified. Genetic mapping showed that most members of the TLH gene family are tightly linked to the telomeres. A physical mapping technique, termed RecA-mediated Achilles' heel cleavage, and cloning and sequencing of two additional telomeres of O-137 associated with the TLH gene family confirmed that most members of the TLH gene family are located within 10 kb from the telomeric repeat. A survey of M. grisea strains from diverse hosts revealed that the gene family is ubiquitously present among rice pathogens, but is absent from almost all isolates of hosts other than rice. The gene family appears to be highly dynamic, undergoing frequent deletion/amplification events. Given the presence of similar helicase gene families in chromosome ends of Saccharomyces cerevisiae and Ustilago maydis, the initial association of helicase genes with fungal telomeres might date back to very early stages of the fungal evolution.

Original languageEnglish (US)
Pages (from-to)103-112
Number of pages10
JournalGenetics
Volume162
Issue number1
StatePublished - Sep 1 2002

Fingerprint

Magnaporthe
Fungi
Telomere
Genes
Fungal Genes
Oryza
Ustilago
Sequence Analysis
Saccharomyces cerevisiae
Organism Cloning
Chromosomes

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Gao, Weimin ; Khang, Chang Hyun ; Park, Sook Young ; Lee, Yong Hwan ; Kang, Seogchan. / Evolution and organization of a highly dynamic, subtelomeric helicase gene family in the rice blast fungus Magnaporthe grisea. In: Genetics. 2002 ; Vol. 162, No. 1. pp. 103-112.
@article{e51e67c8094043ce8eddc130681313bb,
title = "Evolution and organization of a highly dynamic, subtelomeric helicase gene family in the rice blast fungus Magnaporthe grisea",
abstract = "Sequence analysis of a 13-kb telomeric region in O-137, a rice pathogenic isolate of Magnaporthe grisea, uncovered a novel gene, designated TLH1 (telomere-linked helicase 1). The TLH1 gene is a member of a gene family, and the sequences flanking this gene family have also been amplified. Genetic mapping showed that most members of the TLH gene family are tightly linked to the telomeres. A physical mapping technique, termed RecA-mediated Achilles' heel cleavage, and cloning and sequencing of two additional telomeres of O-137 associated with the TLH gene family confirmed that most members of the TLH gene family are located within 10 kb from the telomeric repeat. A survey of M. grisea strains from diverse hosts revealed that the gene family is ubiquitously present among rice pathogens, but is absent from almost all isolates of hosts other than rice. The gene family appears to be highly dynamic, undergoing frequent deletion/amplification events. Given the presence of similar helicase gene families in chromosome ends of Saccharomyces cerevisiae and Ustilago maydis, the initial association of helicase genes with fungal telomeres might date back to very early stages of the fungal evolution.",
author = "Weimin Gao and Khang, {Chang Hyun} and Park, {Sook Young} and Lee, {Yong Hwan} and Seogchan Kang",
year = "2002",
month = "9",
day = "1",
language = "English (US)",
volume = "162",
pages = "103--112",
journal = "Genetics",
issn = "0016-6731",
publisher = "Genetics Society of America",
number = "1",

}

Evolution and organization of a highly dynamic, subtelomeric helicase gene family in the rice blast fungus Magnaporthe grisea. / Gao, Weimin; Khang, Chang Hyun; Park, Sook Young; Lee, Yong Hwan; Kang, Seogchan.

In: Genetics, Vol. 162, No. 1, 01.09.2002, p. 103-112.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Evolution and organization of a highly dynamic, subtelomeric helicase gene family in the rice blast fungus Magnaporthe grisea

AU - Gao, Weimin

AU - Khang, Chang Hyun

AU - Park, Sook Young

AU - Lee, Yong Hwan

AU - Kang, Seogchan

PY - 2002/9/1

Y1 - 2002/9/1

N2 - Sequence analysis of a 13-kb telomeric region in O-137, a rice pathogenic isolate of Magnaporthe grisea, uncovered a novel gene, designated TLH1 (telomere-linked helicase 1). The TLH1 gene is a member of a gene family, and the sequences flanking this gene family have also been amplified. Genetic mapping showed that most members of the TLH gene family are tightly linked to the telomeres. A physical mapping technique, termed RecA-mediated Achilles' heel cleavage, and cloning and sequencing of two additional telomeres of O-137 associated with the TLH gene family confirmed that most members of the TLH gene family are located within 10 kb from the telomeric repeat. A survey of M. grisea strains from diverse hosts revealed that the gene family is ubiquitously present among rice pathogens, but is absent from almost all isolates of hosts other than rice. The gene family appears to be highly dynamic, undergoing frequent deletion/amplification events. Given the presence of similar helicase gene families in chromosome ends of Saccharomyces cerevisiae and Ustilago maydis, the initial association of helicase genes with fungal telomeres might date back to very early stages of the fungal evolution.

AB - Sequence analysis of a 13-kb telomeric region in O-137, a rice pathogenic isolate of Magnaporthe grisea, uncovered a novel gene, designated TLH1 (telomere-linked helicase 1). The TLH1 gene is a member of a gene family, and the sequences flanking this gene family have also been amplified. Genetic mapping showed that most members of the TLH gene family are tightly linked to the telomeres. A physical mapping technique, termed RecA-mediated Achilles' heel cleavage, and cloning and sequencing of two additional telomeres of O-137 associated with the TLH gene family confirmed that most members of the TLH gene family are located within 10 kb from the telomeric repeat. A survey of M. grisea strains from diverse hosts revealed that the gene family is ubiquitously present among rice pathogens, but is absent from almost all isolates of hosts other than rice. The gene family appears to be highly dynamic, undergoing frequent deletion/amplification events. Given the presence of similar helicase gene families in chromosome ends of Saccharomyces cerevisiae and Ustilago maydis, the initial association of helicase genes with fungal telomeres might date back to very early stages of the fungal evolution.

UR - http://www.scopus.com/inward/record.url?scp=0036741619&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036741619&partnerID=8YFLogxK

M3 - Article

C2 - 12242226

AN - SCOPUS:0036741619

VL - 162

SP - 103

EP - 112

JO - Genetics

JF - Genetics

SN - 0016-6731

IS - 1

ER -