Evolution and protein interactions of AP2 proteins in Brassicaceae: Evidence linking development and environmental responses

Liping Zeng, Yue Yin, Chenjiang You, Qianli Pan, Duo Xu, Taijie Jin, Bailong Zhang, Hong Ma

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Plants have evolved a large number of transcription factors (TF), which are enriched among duplicate genes, highlighting their roles in complex regulatory networks. The APETALA2/EREBP-like genes constitute a large plant TF family and participate in development and stress responses. To probe the conservation and divergence of AP2/EREBP genes, we analyzed the duplication patterns of this family in Brassicaceae and identified interacting proteins of representative Arabidopsis AP2/EREBP proteins. We found that many AP2/EREBP duplicates generated early in Brassicaceae history were quickly lost, but many others were retained in all tested Brassicaceae species, suggesting early functional divergence followed by persistent conservation. In addition, the sequences of the AP2 domain and exon numbers were highly conserved in rosids. Furthermore, we used 16 A. thaliana AP2/EREBP proteins as baits in yeast screens and identified 1,970 potential AP2/EREBP-interacting proteins, with a small subset of interactions verified in planta. Many AP2 genes also exhibit reduced expression in an anther-defective mutant, providing a possible link to developmental regulation. The putative AP2-interacting proteins participate in many functions in development and stress responses, including photomorphogenesis, flower development, pathogenesis, drought and cold responses, abscisic acid and auxin signaling. Our results present the AP2/EREBP evolution patterns in Brassicaceae, and support a proposed interaction network of AP2/EREBP proteins and their putative interacting proteins for further study.

Original languageEnglish (US)
Pages (from-to)549-563
Number of pages15
JournalJournal of Integrative Plant Biology
Volume58
Issue number6
DOIs
StatePublished - Jun 1 2016

Fingerprint

Brassicaceae
protein-protein interactions
Genes
Proteins
proteins
Conservation
Transcription Factors
Duplicate Genes
stress response
transcription factors
Abscisic Acid
Indoleacetic Acids
Gene Duplication
Drought
Droughts
duplicate genes
photomorphogenesis
genes
Yeast
Plantae

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Plant Science

Cite this

Zeng, Liping ; Yin, Yue ; You, Chenjiang ; Pan, Qianli ; Xu, Duo ; Jin, Taijie ; Zhang, Bailong ; Ma, Hong. / Evolution and protein interactions of AP2 proteins in Brassicaceae : Evidence linking development and environmental responses. In: Journal of Integrative Plant Biology. 2016 ; Vol. 58, No. 6. pp. 549-563.
@article{da95bfa3a869406bbd092f168d2623c3,
title = "Evolution and protein interactions of AP2 proteins in Brassicaceae: Evidence linking development and environmental responses",
abstract = "Plants have evolved a large number of transcription factors (TF), which are enriched among duplicate genes, highlighting their roles in complex regulatory networks. The APETALA2/EREBP-like genes constitute a large plant TF family and participate in development and stress responses. To probe the conservation and divergence of AP2/EREBP genes, we analyzed the duplication patterns of this family in Brassicaceae and identified interacting proteins of representative Arabidopsis AP2/EREBP proteins. We found that many AP2/EREBP duplicates generated early in Brassicaceae history were quickly lost, but many others were retained in all tested Brassicaceae species, suggesting early functional divergence followed by persistent conservation. In addition, the sequences of the AP2 domain and exon numbers were highly conserved in rosids. Furthermore, we used 16 A. thaliana AP2/EREBP proteins as baits in yeast screens and identified 1,970 potential AP2/EREBP-interacting proteins, with a small subset of interactions verified in planta. Many AP2 genes also exhibit reduced expression in an anther-defective mutant, providing a possible link to developmental regulation. The putative AP2-interacting proteins participate in many functions in development and stress responses, including photomorphogenesis, flower development, pathogenesis, drought and cold responses, abscisic acid and auxin signaling. Our results present the AP2/EREBP evolution patterns in Brassicaceae, and support a proposed interaction network of AP2/EREBP proteins and their putative interacting proteins for further study.",
author = "Liping Zeng and Yue Yin and Chenjiang You and Qianli Pan and Duo Xu and Taijie Jin and Bailong Zhang and Hong Ma",
year = "2016",
month = "6",
day = "1",
doi = "10.1111/jipb.12439",
language = "English (US)",
volume = "58",
pages = "549--563",
journal = "Journal of Integrative Plant Biology",
issn = "1672-9072",
publisher = "Wiley-Blackwell",
number = "6",

}

Evolution and protein interactions of AP2 proteins in Brassicaceae : Evidence linking development and environmental responses. / Zeng, Liping; Yin, Yue; You, Chenjiang; Pan, Qianli; Xu, Duo; Jin, Taijie; Zhang, Bailong; Ma, Hong.

In: Journal of Integrative Plant Biology, Vol. 58, No. 6, 01.06.2016, p. 549-563.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Evolution and protein interactions of AP2 proteins in Brassicaceae

T2 - Evidence linking development and environmental responses

AU - Zeng, Liping

AU - Yin, Yue

AU - You, Chenjiang

AU - Pan, Qianli

AU - Xu, Duo

AU - Jin, Taijie

AU - Zhang, Bailong

AU - Ma, Hong

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Plants have evolved a large number of transcription factors (TF), which are enriched among duplicate genes, highlighting their roles in complex regulatory networks. The APETALA2/EREBP-like genes constitute a large plant TF family and participate in development and stress responses. To probe the conservation and divergence of AP2/EREBP genes, we analyzed the duplication patterns of this family in Brassicaceae and identified interacting proteins of representative Arabidopsis AP2/EREBP proteins. We found that many AP2/EREBP duplicates generated early in Brassicaceae history were quickly lost, but many others were retained in all tested Brassicaceae species, suggesting early functional divergence followed by persistent conservation. In addition, the sequences of the AP2 domain and exon numbers were highly conserved in rosids. Furthermore, we used 16 A. thaliana AP2/EREBP proteins as baits in yeast screens and identified 1,970 potential AP2/EREBP-interacting proteins, with a small subset of interactions verified in planta. Many AP2 genes also exhibit reduced expression in an anther-defective mutant, providing a possible link to developmental regulation. The putative AP2-interacting proteins participate in many functions in development and stress responses, including photomorphogenesis, flower development, pathogenesis, drought and cold responses, abscisic acid and auxin signaling. Our results present the AP2/EREBP evolution patterns in Brassicaceae, and support a proposed interaction network of AP2/EREBP proteins and their putative interacting proteins for further study.

AB - Plants have evolved a large number of transcription factors (TF), which are enriched among duplicate genes, highlighting their roles in complex regulatory networks. The APETALA2/EREBP-like genes constitute a large plant TF family and participate in development and stress responses. To probe the conservation and divergence of AP2/EREBP genes, we analyzed the duplication patterns of this family in Brassicaceae and identified interacting proteins of representative Arabidopsis AP2/EREBP proteins. We found that many AP2/EREBP duplicates generated early in Brassicaceae history were quickly lost, but many others were retained in all tested Brassicaceae species, suggesting early functional divergence followed by persistent conservation. In addition, the sequences of the AP2 domain and exon numbers were highly conserved in rosids. Furthermore, we used 16 A. thaliana AP2/EREBP proteins as baits in yeast screens and identified 1,970 potential AP2/EREBP-interacting proteins, with a small subset of interactions verified in planta. Many AP2 genes also exhibit reduced expression in an anther-defective mutant, providing a possible link to developmental regulation. The putative AP2-interacting proteins participate in many functions in development and stress responses, including photomorphogenesis, flower development, pathogenesis, drought and cold responses, abscisic acid and auxin signaling. Our results present the AP2/EREBP evolution patterns in Brassicaceae, and support a proposed interaction network of AP2/EREBP proteins and their putative interacting proteins for further study.

UR - http://www.scopus.com/inward/record.url?scp=84971473012&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84971473012&partnerID=8YFLogxK

U2 - 10.1111/jipb.12439

DO - 10.1111/jipb.12439

M3 - Article

C2 - 26472270

AN - SCOPUS:84971473012

VL - 58

SP - 549

EP - 563

JO - Journal of Integrative Plant Biology

JF - Journal of Integrative Plant Biology

SN - 1672-9072

IS - 6

ER -