Evolution of genome size and complexity in Pinus

Alison M. Morse, Daniel G. Peterson, M. Nurul Islam-Faridi, Katherine E. Smith, Zenaida Magbanua, Saul A. Garcia, Thomas L. Kubisiak, Henry V. Amerson, John Edward Carlson, C. Dana Nelson, John M. Davis

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

Background: Genome evolution in the gymnosperm lineage of seed plants has given rise to many of the most complex and largest plant genomes, however the elements involved are poorly understood. Methodology/Principal Findings: Gymny is a previously undescribed retrotransposon family in Pinus that is related to Athila elements in Arabidopsis. Gymny elements are dispersed throughout the modern Pinus genome and occupy a physical space at least the size of the Arabidopsis thaliana genome. In contrast to previously described retroelements in Pinus, the Gymny family was amplified or introduced after the divergence of pine and spruce (Picea). If retrotransposon expansions are responsible for genome size differences within the Pinaceae, as they are in angiosperms, then they have yet to be identified. In contrast, molecular divergence of Gymny retrotransposons together with other families of retrotransposons can account for the large genome complexity of pines along with protein-coding genic DNA, as revealed by massively parallel DNA sequence analysis of Cot fractionated genomic DNA. Conclusions/Significance: Most of the enormous genome complexity of pines can be explained by divergence of retrotransposons, however the elements responsible for genome size variation are yet to be identified. Genomic resources for Pinus including those reported here should assist in further defining whether and how the roles of retrotransposons differ in the evolution of angiosperm and gymnosperm genomes.

Original languageEnglish (US)
Article numbere4332
JournalPloS one
Volume4
Issue number2
DOIs
StatePublished - Feb 5 2009

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Genome Size
Pinus
Retroelements
retrotransposons
Genes
Genome
genome
Gymnosperms
Angiosperms
Arabidopsis
Pinaceae
Picea
Plant Genome
Angiospermae
DNA
genomics
DNA Sequence Analysis
DNA sequences
Seeds
Spermatophytina

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Morse, A. M., Peterson, D. G., Islam-Faridi, M. N., Smith, K. E., Magbanua, Z., Garcia, S. A., ... Davis, J. M. (2009). Evolution of genome size and complexity in Pinus. PloS one, 4(2), [e4332]. https://doi.org/10.1371/journal.pone.0004332
Morse, Alison M. ; Peterson, Daniel G. ; Islam-Faridi, M. Nurul ; Smith, Katherine E. ; Magbanua, Zenaida ; Garcia, Saul A. ; Kubisiak, Thomas L. ; Amerson, Henry V. ; Carlson, John Edward ; Nelson, C. Dana ; Davis, John M. / Evolution of genome size and complexity in Pinus. In: PloS one. 2009 ; Vol. 4, No. 2.
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Morse, AM, Peterson, DG, Islam-Faridi, MN, Smith, KE, Magbanua, Z, Garcia, SA, Kubisiak, TL, Amerson, HV, Carlson, JE, Nelson, CD & Davis, JM 2009, 'Evolution of genome size and complexity in Pinus', PloS one, vol. 4, no. 2, e4332. https://doi.org/10.1371/journal.pone.0004332

Evolution of genome size and complexity in Pinus. / Morse, Alison M.; Peterson, Daniel G.; Islam-Faridi, M. Nurul; Smith, Katherine E.; Magbanua, Zenaida; Garcia, Saul A.; Kubisiak, Thomas L.; Amerson, Henry V.; Carlson, John Edward; Nelson, C. Dana; Davis, John M.

In: PloS one, Vol. 4, No. 2, e4332, 05.02.2009.

Research output: Contribution to journalArticle

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Morse AM, Peterson DG, Islam-Faridi MN, Smith KE, Magbanua Z, Garcia SA et al. Evolution of genome size and complexity in Pinus. PloS one. 2009 Feb 5;4(2). e4332. https://doi.org/10.1371/journal.pone.0004332