Evolutionary potential of the extrinsic incubation period of dengue virus in Aedes aegypti

Yixin H. Ye, Stephen F. Chenoweth, Alison M. Carrasco, Scott L. Allen, Francesca D. Frentiu, Andrew F. van den Hurk, Nigel W. Beebe, Elizabeth A. McGraw

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Dengue fever is the most common arboviral disease worldwide. It is caused by dengue viruses (DENV) and the mosquito Aedes aegypti is its primary vector. One of the most powerful determinants of a mosquito's ability to transmit DENV is the length of the extrinsic incubation period (EIP), the time it takes for a virus to be transmitted by a mosquito after consuming an infected blood meal. Here, we repeatedly measured DENV load in the saliva of individual mosquitoes over their lifetime and used this in combination with a breeding design to determine the extent to which EIP might respond to the evolutionary forces of drift and selection. We demonstrated that genetic variation among mosquitoes contributes significantly to transmission potential and length of EIP. We reveal that shorter EIP is genetically correlated with reduced mosquito lifespan, highlighting negative life-history consequences for virus-infected mosquitoes. This work highlights the capacity for local genetic variation in mosquito populations to evolve and to dramatically affect the nature of human outbreaks. It also provides the impetus for isolating mosquito genes that determine EIP. More broadly, our dual experimental approach offers new opportunities for studying the evolutionary potential of transmission traits in other vector/pathogen systems.

Original languageEnglish (US)
Pages (from-to)2459-2469
Number of pages11
JournalEvolution
Volume70
Issue number11
DOIs
StatePublished - Nov 1 2016

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Agricultural and Biological Sciences(all)

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    Ye, Y. H., Chenoweth, S. F., Carrasco, A. M., Allen, S. L., Frentiu, F. D., van den Hurk, A. F., Beebe, N. W., & McGraw, E. A. (2016). Evolutionary potential of the extrinsic incubation period of dengue virus in Aedes aegypti. Evolution, 70(11), 2459-2469. https://doi.org/10.1111/evo.13039