Abstract
The Fe(II)- and α-ketoglutarate-dependent dioxygenases catalyze hydroxylation reactions of considerable biomedical and environmental significance. Recently, the first oxidized iron intermediate in the reaction of a member of this family, taurine:α-ketoglutarate dioxygenase (TauD), was detected and shown to be a high-spin Fe(IV) complex. In this study we have used X-ray absorption spectroscopy to demonstrate the presence of a short (1.62 Å) interaction between the iron and one of its ligands in the Fe(IV) intermediate but not in the Fe(II) starting complex. The detection of this interaction strongly corroborates the hypothesis that the intermediate contains an Fe=O structural motif.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 8108-8109 |
| Number of pages | 2 |
| Journal | Journal of the American Chemical Society |
| Volume | 126 |
| Issue number | 26 |
| DOIs | |
| State | Published - Jul 7 2004 |
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All Science Journal Classification (ASJC) codes
- Catalysis
- Chemistry(all)
- Biochemistry
- Colloid and Surface Chemistry
Cite this
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EXAFS spectroscopic evidence for an Fe=O unit in the Fe(IV) intermediate observed during oxygen activation by taurine:α-ketoglutarate dioxygenase. / Riggs-Gelasco, Pamela J.; Price, John C.; Guyer, Robert B.; Brehm, Jessica H.; Barr, Eric W.; Bollinger, Jr., Joseph M.; Krebs, Carsten.
In: Journal of the American Chemical Society, Vol. 126, No. 26, 07.07.2004, p. 8108-8109.Research output: Contribution to journal › Article
TY - JOUR
T1 - EXAFS spectroscopic evidence for an Fe=O unit in the Fe(IV) intermediate observed during oxygen activation by taurine:α-ketoglutarate dioxygenase
AU - Riggs-Gelasco, Pamela J.
AU - Price, John C.
AU - Guyer, Robert B.
AU - Brehm, Jessica H.
AU - Barr, Eric W.
AU - Bollinger, Jr., Joseph M.
AU - Krebs, Carsten
PY - 2004/7/7
Y1 - 2004/7/7
N2 - The Fe(II)- and α-ketoglutarate-dependent dioxygenases catalyze hydroxylation reactions of considerable biomedical and environmental significance. Recently, the first oxidized iron intermediate in the reaction of a member of this family, taurine:α-ketoglutarate dioxygenase (TauD), was detected and shown to be a high-spin Fe(IV) complex. In this study we have used X-ray absorption spectroscopy to demonstrate the presence of a short (1.62 Å) interaction between the iron and one of its ligands in the Fe(IV) intermediate but not in the Fe(II) starting complex. The detection of this interaction strongly corroborates the hypothesis that the intermediate contains an Fe=O structural motif.
AB - The Fe(II)- and α-ketoglutarate-dependent dioxygenases catalyze hydroxylation reactions of considerable biomedical and environmental significance. Recently, the first oxidized iron intermediate in the reaction of a member of this family, taurine:α-ketoglutarate dioxygenase (TauD), was detected and shown to be a high-spin Fe(IV) complex. In this study we have used X-ray absorption spectroscopy to demonstrate the presence of a short (1.62 Å) interaction between the iron and one of its ligands in the Fe(IV) intermediate but not in the Fe(II) starting complex. The detection of this interaction strongly corroborates the hypothesis that the intermediate contains an Fe=O structural motif.
UR - http://www.scopus.com/inward/record.url?scp=3042770458&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3042770458&partnerID=8YFLogxK
U2 - 10.1021/ja048255q
DO - 10.1021/ja048255q
M3 - Article
C2 - 15225039
AN - SCOPUS:3042770458
VL - 126
SP - 8108
EP - 8109
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
SN - 0002-7863
IS - 26
ER -