Examining kinesin processivity within a general gating framework

Johan O.L. Andreasson, Bojan Milic, Geng Yuan Chen, Nicholas R. Guydosh, William O. Hancock, Steven M. Block

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Kinesin-1 is a dimeric motor that transports cargo along microtubules, taking 8.2-nm steps in a hand-over-hand fashion. The ATP hydrolysis cycles of its two heads are maintained out of phase by a series of gating mechanisms, which lead to processive runs averaging ~1 μm. A key structural element for inter-head coordination is the neck linker (NL), which connects the heads to the stalk. To examine the role of the NL in regulating stepping, we investigated NL mutants of various lengths using single-molecule optical trapping and bulk fluorescence approaches in the context of a general framework for gating. Our results show that, although inter-head tension enhances motor velocity, it is crucial neither for inter-head coordination nor for rapid rear-head release. Furthermore, cysteine-light mutants do not produce wild-type motility under load. We conclude that kinesin-1 is primarily front-head gated, and that NL length is tuned to enhance unidirectional processivity and velocity.

Original languageEnglish (US)
Pages (from-to)1-44
Number of pages44
JournaleLife
Volume2015
Issue number4
DOIs
StatePublished - Apr 22 2015

Fingerprint

Kinesin
Head
Neck
Cysteine
Hydrolysis
Adenosine Triphosphate
Fluorescence
Molecules
Hand
Optical Tweezers
Microtubules
Light

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Andreasson, J. O. L., Milic, B., Chen, G. Y., Guydosh, N. R., Hancock, W. O., & Block, S. M. (2015). Examining kinesin processivity within a general gating framework. eLife, 2015(4), 1-44. https://doi.org/10.7554/eLife.07403
Andreasson, Johan O.L. ; Milic, Bojan ; Chen, Geng Yuan ; Guydosh, Nicholas R. ; Hancock, William O. ; Block, Steven M. / Examining kinesin processivity within a general gating framework. In: eLife. 2015 ; Vol. 2015, No. 4. pp. 1-44.
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Andreasson, JOL, Milic, B, Chen, GY, Guydosh, NR, Hancock, WO & Block, SM 2015, 'Examining kinesin processivity within a general gating framework', eLife, vol. 2015, no. 4, pp. 1-44. https://doi.org/10.7554/eLife.07403

Examining kinesin processivity within a general gating framework. / Andreasson, Johan O.L.; Milic, Bojan; Chen, Geng Yuan; Guydosh, Nicholas R.; Hancock, William O.; Block, Steven M.

In: eLife, Vol. 2015, No. 4, 22.04.2015, p. 1-44.

Research output: Contribution to journalArticle

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Andreasson JOL, Milic B, Chen GY, Guydosh NR, Hancock WO, Block SM. Examining kinesin processivity within a general gating framework. eLife. 2015 Apr 22;2015(4):1-44. https://doi.org/10.7554/eLife.07403