Exocrine pancreas development in zebrafish

Nelson S. Yee, Kristin Lorent, Michael Pack

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Although many of the genes that regulate development of the endocrine pancreas have been identified, comparatively little is known about how the exocrine pancreas forms. Previous studies have shown that exocrine pancreas development may be modeled in zebrafish. However, the timing and mechanism of acinar and ductal differentiation and morphogenesis have not been described. Here, we characterize zebrafish exocrine pancreas development in wild type and mutant larvae using histological, immunohistochemical and ultrastructural analyses. These data allow us to identify two stages of zebrafish exocrine development. During the first stage, the exocrine anlage forms from rostral endodermal cells. During the second stage, protodifferentiated progenitor cells undergo terminal differentiation followed by acinar gland and duct morphogenesis. Immunohistochemical analyses support a model in which the intrapancreatic ductal system develops from progenitors that join to form a contiguous network rather than by branching morphogenesis of the pancreatic epithelium, as described for mammals. Contemporaneous appearance of acinar glands and ducts in developing larvae and their disruption in pancreatic mutants suggest that common molecular pathways may regulate gland and duct morphogenesis and differentiation of their constituent cells. By contrast, analyses of mind bomb mutants and jagged morpholino-injected larvae suggest that Notch signaling principally regulates ductal differentiation of bipotential exocrine progenitors.

Original languageEnglish (US)
Pages (from-to)84-101
Number of pages18
JournalDevelopmental biology
Volume284
Issue number1
DOIs
StatePublished - Aug 1 2005

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Exocrine Pancreas
Zebrafish
Morphogenesis
Larva
Bombs
Morpholinos
Islets of Langerhans
Mammals
Stem Cells
Epithelium
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Yee, Nelson S. ; Lorent, Kristin ; Pack, Michael. / Exocrine pancreas development in zebrafish. In: Developmental biology. 2005 ; Vol. 284, No. 1. pp. 84-101.
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Exocrine pancreas development in zebrafish. / Yee, Nelson S.; Lorent, Kristin; Pack, Michael.

In: Developmental biology, Vol. 284, No. 1, 01.08.2005, p. 84-101.

Research output: Contribution to journalArticle

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