Expansion of natural (NK1+) T cells that express αβ T cell receptors in transporters associated with antigen presentation-1 null and thymus leukemia antigen positive mice

Sebastian Joyce, Izumi Negishi, Alina Boesteanu, A. Dharshan DeSilva, Padmanee Sharma, Michael J. Chorney, Dennis Y. Loh, Luc Van Kaer

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

Thymic selection of natural killer-11 natural cells that express αβ T cell receptors requires a conserved β2-microglobulin-associated molecule, presumably CD1d, displayed by CD4+8+ thymocytes. Here we demonstrate that positive selection of natural T cells occurs independent of transporters associated with antigen presentation-1 (TAP-1) function. Moreover, natural T cells in TAP-1(0/0) mice are numerically expanded. Several H-2 class Ib molecules function in a TAP-independent manner, suggesting that if expressed in TAP-1(0/0) thymocytes, they could play a role in natural T cell development. Of these class Ib molecules, H-2TL is expressed by TAP-1(0/0) thymocytes. Moreover, we find that thymi of TL+ mice congenic or transgenic for H-2T18 also have a numerically expanded natural T cell repertoire compared with TL+ mice. This expansion, as in TAP-1(0/0) thymi, is evident in each of the limited T cell receptor Vβ chains expressed by natural T cells, suggesting tat TL and CD1d impact similar repertoires. Thus TL, in addition to CD1d, plays a role in natural T cell development.

Original languageEnglish (US)
Pages (from-to)1579-1584
Number of pages6
JournalJournal of Experimental Medicine
Volume184
Issue number4
DOIs
StatePublished - Oct 1 1996

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Expansion of natural (NK1<sup>+</sup>) T cells that express αβ T cell receptors in transporters associated with antigen presentation-1 null and thymus leukemia antigen positive mice'. Together they form a unique fingerprint.

  • Cite this