Experimental T-2 toxicosis in swine

III. Morphologic changes following intravascular administration of t-2 toxin

Victor F. Pang, Roseanne M. Lorenzana, Val Richard Beasley, William B. Buck, Wanda M. Haschek

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Experimental T-2 Toxicosis in Swine. III. Morphologic Changes following Intravascular Administration of T-2 Toxin. PANG, V. F., LORENZANA, R. M., BEASLEY, V. R., BUCK, W. B., and HASCHEK, W. M. (1987) Fundam. Appl. Toxicol. 8, 298-309. The gross and microscopic changes in swine following a single intravascular (iv) dose of T-2 toxin are described and evaluated quantitatively. T-2 toxin, in 70% ethanol, was given iv at 0 (5 pigs), 0.6 (5 pigs), 1.2(1 pig), 4.8 (5 pigs), or 5.4 (2 pigs) mg/kg to 40 to 60 kg female crossbred pigs. The 4.8 and 5.4 mg/kg group pigs died between 5 and 10.5 hr after treatment, while the 0, 0.6, and 1.2 mg/kg pigs were killed at 24, 24, and 12 hrs after treatment, respectively. Morphologic examination was performed at the gross and light microscopic levels. In addition, a quantitative evaluation of microscopic changes present in lymphoid tissues and intestinal tract was performed using a semiquantitative scoring system. Gross lesions in the T-2-treated pigs consisted of edema, congestion, and hemorrhage of the lymph nodes and pancreas; congestion and hemorrhage of the gastrointestinal mucosa, subendocardium, adrenal gland, and meninges; and edema of the gall bladder. Histologic examination confirmed the gross observations. Additional microscopic lesions included widespread degeneration and necrosis of the lymphoid tissues as well as of the surface and crypt epithelium of the gastrointestinal mucosa; mild scattered necrosis of pancreatic acinar cells, myocardium, bone marrow cells, adrenal cortical cells, and tubular epithelium of renal medulla; and mild interstitial pneumonia. A dose-dependent increase in lesion severity was observed except for the pancreatic lesion which was slightly more apparent in the pigs from the 0.6 mg/kg group. These findings indicate that (1) T-2 toxin-induced lesions in the lymphoid tissues and gastrointestinal tract of pigs are similar to those of other species, (2) the pancreas and heart should be considered as additional target organs in the pig, and (3) both rapidly dividing cells and those with little or no turnover are damaged by T-2 toxin.

Original languageEnglish (US)
Pages (from-to)298-309
Number of pages12
JournalToxicological Sciences
Volume8
Issue number3
DOIs
StatePublished - Mar 1 1987

Fingerprint

T-2 Toxin
Swine
Tissue
Lymphoid Tissue
Bone
Ethanol
Cells
Pancreas
Edema
Mucous Membrane
Necrosis
Epithelium
Meninges
Gastrointestinal Hemorrhage
Acinar Cells
Interstitial Lung Diseases
Adrenal Glands

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Pang, Victor F. ; Lorenzana, Roseanne M. ; Beasley, Val Richard ; Buck, William B. ; Haschek, Wanda M. / Experimental T-2 toxicosis in swine : III. Morphologic changes following intravascular administration of t-2 toxin. In: Toxicological Sciences. 1987 ; Vol. 8, No. 3. pp. 298-309.
@article{47cad98eb99745ce95d626bd4c2a6313,
title = "Experimental T-2 toxicosis in swine: III. Morphologic changes following intravascular administration of t-2 toxin",
abstract = "Experimental T-2 Toxicosis in Swine. III. Morphologic Changes following Intravascular Administration of T-2 Toxin. PANG, V. F., LORENZANA, R. M., BEASLEY, V. R., BUCK, W. B., and HASCHEK, W. M. (1987) Fundam. Appl. Toxicol. 8, 298-309. The gross and microscopic changes in swine following a single intravascular (iv) dose of T-2 toxin are described and evaluated quantitatively. T-2 toxin, in 70{\%} ethanol, was given iv at 0 (5 pigs), 0.6 (5 pigs), 1.2(1 pig), 4.8 (5 pigs), or 5.4 (2 pigs) mg/kg to 40 to 60 kg female crossbred pigs. The 4.8 and 5.4 mg/kg group pigs died between 5 and 10.5 hr after treatment, while the 0, 0.6, and 1.2 mg/kg pigs were killed at 24, 24, and 12 hrs after treatment, respectively. Morphologic examination was performed at the gross and light microscopic levels. In addition, a quantitative evaluation of microscopic changes present in lymphoid tissues and intestinal tract was performed using a semiquantitative scoring system. Gross lesions in the T-2-treated pigs consisted of edema, congestion, and hemorrhage of the lymph nodes and pancreas; congestion and hemorrhage of the gastrointestinal mucosa, subendocardium, adrenal gland, and meninges; and edema of the gall bladder. Histologic examination confirmed the gross observations. Additional microscopic lesions included widespread degeneration and necrosis of the lymphoid tissues as well as of the surface and crypt epithelium of the gastrointestinal mucosa; mild scattered necrosis of pancreatic acinar cells, myocardium, bone marrow cells, adrenal cortical cells, and tubular epithelium of renal medulla; and mild interstitial pneumonia. A dose-dependent increase in lesion severity was observed except for the pancreatic lesion which was slightly more apparent in the pigs from the 0.6 mg/kg group. These findings indicate that (1) T-2 toxin-induced lesions in the lymphoid tissues and gastrointestinal tract of pigs are similar to those of other species, (2) the pancreas and heart should be considered as additional target organs in the pig, and (3) both rapidly dividing cells and those with little or no turnover are damaged by T-2 toxin.",
author = "Pang, {Victor F.} and Lorenzana, {Roseanne M.} and Beasley, {Val Richard} and Buck, {William B.} and Haschek, {Wanda M.}",
year = "1987",
month = "3",
day = "1",
doi = "10.1093/toxsci/8.3.298",
language = "English (US)",
volume = "8",
pages = "298--309",
journal = "Toxicological Sciences",
issn = "1096-6080",
publisher = "Oxford University Press",
number = "3",

}

Experimental T-2 toxicosis in swine : III. Morphologic changes following intravascular administration of t-2 toxin. / Pang, Victor F.; Lorenzana, Roseanne M.; Beasley, Val Richard; Buck, William B.; Haschek, Wanda M.

In: Toxicological Sciences, Vol. 8, No. 3, 01.03.1987, p. 298-309.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Experimental T-2 toxicosis in swine

T2 - III. Morphologic changes following intravascular administration of t-2 toxin

AU - Pang, Victor F.

AU - Lorenzana, Roseanne M.

AU - Beasley, Val Richard

AU - Buck, William B.

AU - Haschek, Wanda M.

PY - 1987/3/1

Y1 - 1987/3/1

N2 - Experimental T-2 Toxicosis in Swine. III. Morphologic Changes following Intravascular Administration of T-2 Toxin. PANG, V. F., LORENZANA, R. M., BEASLEY, V. R., BUCK, W. B., and HASCHEK, W. M. (1987) Fundam. Appl. Toxicol. 8, 298-309. The gross and microscopic changes in swine following a single intravascular (iv) dose of T-2 toxin are described and evaluated quantitatively. T-2 toxin, in 70% ethanol, was given iv at 0 (5 pigs), 0.6 (5 pigs), 1.2(1 pig), 4.8 (5 pigs), or 5.4 (2 pigs) mg/kg to 40 to 60 kg female crossbred pigs. The 4.8 and 5.4 mg/kg group pigs died between 5 and 10.5 hr after treatment, while the 0, 0.6, and 1.2 mg/kg pigs were killed at 24, 24, and 12 hrs after treatment, respectively. Morphologic examination was performed at the gross and light microscopic levels. In addition, a quantitative evaluation of microscopic changes present in lymphoid tissues and intestinal tract was performed using a semiquantitative scoring system. Gross lesions in the T-2-treated pigs consisted of edema, congestion, and hemorrhage of the lymph nodes and pancreas; congestion and hemorrhage of the gastrointestinal mucosa, subendocardium, adrenal gland, and meninges; and edema of the gall bladder. Histologic examination confirmed the gross observations. Additional microscopic lesions included widespread degeneration and necrosis of the lymphoid tissues as well as of the surface and crypt epithelium of the gastrointestinal mucosa; mild scattered necrosis of pancreatic acinar cells, myocardium, bone marrow cells, adrenal cortical cells, and tubular epithelium of renal medulla; and mild interstitial pneumonia. A dose-dependent increase in lesion severity was observed except for the pancreatic lesion which was slightly more apparent in the pigs from the 0.6 mg/kg group. These findings indicate that (1) T-2 toxin-induced lesions in the lymphoid tissues and gastrointestinal tract of pigs are similar to those of other species, (2) the pancreas and heart should be considered as additional target organs in the pig, and (3) both rapidly dividing cells and those with little or no turnover are damaged by T-2 toxin.

AB - Experimental T-2 Toxicosis in Swine. III. Morphologic Changes following Intravascular Administration of T-2 Toxin. PANG, V. F., LORENZANA, R. M., BEASLEY, V. R., BUCK, W. B., and HASCHEK, W. M. (1987) Fundam. Appl. Toxicol. 8, 298-309. The gross and microscopic changes in swine following a single intravascular (iv) dose of T-2 toxin are described and evaluated quantitatively. T-2 toxin, in 70% ethanol, was given iv at 0 (5 pigs), 0.6 (5 pigs), 1.2(1 pig), 4.8 (5 pigs), or 5.4 (2 pigs) mg/kg to 40 to 60 kg female crossbred pigs. The 4.8 and 5.4 mg/kg group pigs died between 5 and 10.5 hr after treatment, while the 0, 0.6, and 1.2 mg/kg pigs were killed at 24, 24, and 12 hrs after treatment, respectively. Morphologic examination was performed at the gross and light microscopic levels. In addition, a quantitative evaluation of microscopic changes present in lymphoid tissues and intestinal tract was performed using a semiquantitative scoring system. Gross lesions in the T-2-treated pigs consisted of edema, congestion, and hemorrhage of the lymph nodes and pancreas; congestion and hemorrhage of the gastrointestinal mucosa, subendocardium, adrenal gland, and meninges; and edema of the gall bladder. Histologic examination confirmed the gross observations. Additional microscopic lesions included widespread degeneration and necrosis of the lymphoid tissues as well as of the surface and crypt epithelium of the gastrointestinal mucosa; mild scattered necrosis of pancreatic acinar cells, myocardium, bone marrow cells, adrenal cortical cells, and tubular epithelium of renal medulla; and mild interstitial pneumonia. A dose-dependent increase in lesion severity was observed except for the pancreatic lesion which was slightly more apparent in the pigs from the 0.6 mg/kg group. These findings indicate that (1) T-2 toxin-induced lesions in the lymphoid tissues and gastrointestinal tract of pigs are similar to those of other species, (2) the pancreas and heart should be considered as additional target organs in the pig, and (3) both rapidly dividing cells and those with little or no turnover are damaged by T-2 toxin.

UR - http://www.scopus.com/inward/record.url?scp=77957188544&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77957188544&partnerID=8YFLogxK

U2 - 10.1093/toxsci/8.3.298

DO - 10.1093/toxsci/8.3.298

M3 - Article

VL - 8

SP - 298

EP - 309

JO - Toxicological Sciences

JF - Toxicological Sciences

SN - 1096-6080

IS - 3

ER -