Exploration of the bovine colostrum proteome and effects of heat treatment time on colostrum protein profile

R. Tacoma, S. L. Gelsinger, Y. W. Lam, R. A. Scuderi, D. B. Ebenstein, A. J. Heinrichs, S. L. Greenwood

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Heat treatment of colostrum is performed on modern dairy farms to reduce pathogenic contamination before hand-feeding the colostrum to newborn calves; however, limited data are available concerning effects of heat treatment on biologically active proteins in colostrum. The objective of this exploratory study was to investigate effects of heat treatment and length of heat treatment on colostrum protein profile. Colostrum samples were collected from Holstein cows within 12 h after parturition and assigned to the following groups: heat treatment at 60°C for 0 (untreated control), 30, 60, or 90 min. Samples were fractionated using acid precipitation, followed by ultracentrifugation and ProteoMiner (Bio-Rad Laboratories, Hercules, CA) treatment, and tandem-mass tagging was used to comparatively assess the low abundance protein profile. A total of 162 proteins were identified with more than 2 peptides in the low abundance protein enriched fraction. Of these, 62 differed in abundance by more than 2-fold in heat-treated samples compared with the unheated control. The majority of proteins affected by heat treatment were involved in immunity, enzyme function, and transport-related processes; affected proteins included lactadherin, chitinase-3-like protein 1, and complement component C9. These results provide a foundation for further research to determine optimum heat treatment practices to ensure newborn calves are fed colostrum-containing proteins with the highest nutritional and biological value.

Original languageEnglish (US)
Pages (from-to)9392-9401
Number of pages10
JournalJournal of dairy science
Volume100
Issue number11
DOIs
StatePublished - Nov 2017

Fingerprint

cow colostrum
Colostrum
Proteome
proteome
colostrum
Hot Temperature
heat treatment
Proteins
proteins
Complement C9
lactadherin
neonates
calves
Ultracentrifugation
Nutritive Value
ultracentrifugation
acid deposition
chitinase
sampling
Immunity

All Science Journal Classification (ASJC) codes

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Tacoma, R., Gelsinger, S. L., Lam, Y. W., Scuderi, R. A., Ebenstein, D. B., Heinrichs, A. J., & Greenwood, S. L. (2017). Exploration of the bovine colostrum proteome and effects of heat treatment time on colostrum protein profile. Journal of dairy science, 100(11), 9392-9401. https://doi.org/10.3168/jds.2017-13211
Tacoma, R. ; Gelsinger, S. L. ; Lam, Y. W. ; Scuderi, R. A. ; Ebenstein, D. B. ; Heinrichs, A. J. ; Greenwood, S. L. / Exploration of the bovine colostrum proteome and effects of heat treatment time on colostrum protein profile. In: Journal of dairy science. 2017 ; Vol. 100, No. 11. pp. 9392-9401.
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Exploration of the bovine colostrum proteome and effects of heat treatment time on colostrum protein profile. / Tacoma, R.; Gelsinger, S. L.; Lam, Y. W.; Scuderi, R. A.; Ebenstein, D. B.; Heinrichs, A. J.; Greenwood, S. L.

In: Journal of dairy science, Vol. 100, No. 11, 11.2017, p. 9392-9401.

Research output: Contribution to journalArticle

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